Measuring bacterial activity and community composition at high hydrostatic pressure using a novel experimental approach: a pilot study

• Published in: FEMS microbiology ecology Vol. 91; no. 5; p. 1
• Main Authors: Wannicke, Nicola, Frindte, Katharina, Gust, Giselher, Liskow, Iris, Wacker, Alexander, Meyer, Andreas, Grossart, Hans-Peter
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.05.2015
• Subjects: Adaptation, Physiological - physiology; Alteromonadaceae - metabolism; Alteromonadaceae - physiology; Bacteria; Biomass; Cell culture; Communities; Community composition; Composition; Decompression; Deep sea; Deep sea environments; Ecology; Environmental aspects; Fatty Acids - metabolism; High pressure; Hydrostatic Pressure; Isotope Labeling; Microbial colonies; Microbiology; Multiplication; Observations; Ocean models; Oceans and Seas; Phospholipids - metabolism; Photobacterium - metabolism; Photobacterium - physiology; Physiological aspects; Pilot Projects; Pressure; Protein composition; Germany; stable isotopes; deep-sea bacterial community; piezophilic bacteria; membrane fatty acids; bacterial production; pressure chamber; hydrostatic pressure

In this pilot study, we describe a high-pressure incubation system allowing multiple subsampling of a pressurized culture without decompression. The system was tested using one piezophilic (Photobacterium profundum), one piezotolerant (Colwellia maris) bacterial strain and a decompressed sample from...