In Vitro Glycoengineering of IgG1 and Its Effect on Fc Receptor Binding and ADCC Activity

• Published in: PloS one Vol. 10; no. 8; p. e0134949
• Main Authors: Thomann, Marco, Schlothauer, Tilman, Dashivets, Tetyana, Malik, Sebastian, Avenal, Cecile, Bulau, Patrick, Rüger, Petra, Reusch, Dietmar
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.08.2015; Public Library of Science (PLoS)
• Subjects: Acids; Affinity chromatography; Antibody-Dependent Cell Cytotoxicity; Binding; Binding Sites; Biochemistry; Bioengineering; Biological activity; Biological effects; Chromatography; Enzymes; Fc receptors; Galactose - metabolism; Gene Expression; Glycan; Glycosylation; Glycosyltransferase; HEK293 Cells; Humans; Immunoglobulin G; Immunoglobulin G - chemistry; Immunoglobulin G - genetics; Immunoglobulin G - metabolism; Immunoglobulins; Immunology; Molecular biology; Monoclonal antibodies; Polysaccharides; Protein Binding; Protein Engineering; Proteins; Receptor, Epidermal Growth Factor - genetics; Receptor, Epidermal Growth Factor - metabolism; Receptors; Receptors, IgG - chemistry; Receptors, IgG - genetics; Receptors, IgG - metabolism; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Sialic Acids - metabolism; Sialyltransferases - genetics; Sialyltransferases - metabolism; Studies; Sugar; Surface plasmon resonance; Germany

The importance and effect of Fc glycosylation of monoclonal antibodies with regard to biological activity is widely discussed and has been investigated in numerous studies. Fc glycosylation of monoclonal antibodies from current production systems is subject to batch-to-batch variability. If there ar...