The hPLIC Proteins May Provide a Link between the Ubiquitination Machinery and the Proteasome

• Published in: Molecular cell Vol. 6; no. 2; pp. 409 - 419
• Main Authors: Kleijnen, Maurits F., Shih, Alan H., Zhou, Pengbo, Kumar, Sushant, Soccio, Raymond E., Kedersha, Nancy L., Gill, Grace, Howley, Peter M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2000
• Subjects: Amino Acid Sequence; Animals; Carrier Proteins; Cell Cycle Proteins; Cells, Cultured; Cysteine Endopeptidases - metabolism; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Gene Expression Regulation - drug effects; Humans; Keratinocytes - cytology; Keratinocytes - metabolism; Male; Mice; Molecular Sequence Data; Multienzyme Complexes - metabolism; Proteasome Endopeptidase Complex; Recombinant Proteins - metabolism; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Skin - cytology; Transfection; Tumor Necrosis Factor-alpha - pharmacology; Ubiquitins - chemistry; Ubiquitins - genetics; Ubiquitins - metabolism

Although there is a binding site on the proteasome for the polyubiquitin chains attached to degradation substrates by the ubiquitination machinery, it is currently unclear whether in vivo the activities of the ubiquitination machinery and the proteasome are coupled. Here we show that two human homol...