Comparative Sequencing of the Serine-Aspartate Repeat-Encoding Region of the Clumping Factor B Gene (clfB) for Resolution within Clonal Groups of Staphylococcus aureus

• Published in: Journal of Clinical Microbiology Vol. 43; no. 8; pp. 3985 - 3994
• Main Authors: Koreen, Larry, Ramaswamy, Srinivas V, Naidich, Steven, Koreen, Irina V, Graff, Gavin R, Graviss, Edward A, Kreiswirth, Barry N
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2005; American Society for Microbiology (ASM)
• Subjects: Adhesins, Bacterial - chemistry; Adhesins, Bacterial - genetics; Amino Acid Sequence; Aspartic Acid; Bacterial Typing Techniques; Bacteriology; Base Sequence; Biological and medical sciences; Epidemiology; Evolution, Molecular; Fundamental and applied biological sciences. Psychology; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Molecular Sequence Data; Repetitive Sequences, Amino Acid; Reproducibility of Results; Serine; Staphylococcus aureus - classification; Staphylococcus aureus - genetics; Gene; Microbiology; Serine; Bacteria; Micrococcales; Micrococcaceae; Sequencing; Staphylococcus aureus

Moleculartechniques such as spa typing and multilocus sequence typinguse DNA sequence data for differentiating Staphylococcusaureus isolates. Although spa typing is capable ofdetecting both genetic micro- and macrovariation, it has lessdiscriminatory power than the more labor-intensive pulsed-field...