Comparative Analysis of Substrate-Free Cultured Oral Mucosal Epithelial Cell Sheets from Cells of Subjects with and without Stevens—Johnson Syndrome for Use in Ocular Surface Reconstruction

• Published in: PloS one Vol. 11; no. 1; p. e0147548
• Main Authors: Kim, Yun Hee, Kim, Dong Hyun, Shin, Eun Jung, Lee, Hyun Ju, Wee, Won Ryang, Jeon, Saewha, Kim, Mee Kum
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.01.2016; Public Library of Science (PLoS)
• Subjects: Adolescent; Adult; Biology and Life Sciences; Biomarkers; Biomedical research; Cell migration; Cell Movement; Cell Proliferation; Cells, Cultured; Comparative analysis; Cytokines; Cytokines - metabolism; Epidermal growth factor; Epithelial cells; Epithelium, Corneal - pathology; Eye (anatomy); Female; Growth factors; Hospitals; Humans; Immunology; In vivo methods and tests; Inflammation; Keratins - metabolism; Laboratories; Male; Medicine; Medicine and Health Sciences; Middle Aged; Mouth Mucosa - cytology; Mouth Mucosa - metabolism; Mouth Mucosa - pathology; Mucosa; Mucous membrane; Rabbits; Regeneration; Research and Analysis Methods; Rodents; Sheets; Skin diseases; Stem cell transplantation; Stem cells; Stem Cells - cytology; Stevens-Johnson syndrome; Stevens-Johnson Syndrome - pathology; Substrates; Transplantation; Variance analysis; Vascular endothelial growth factor; Young Adult

To compare the regenerative potential of cultured oral mucosal epithelial cells sheets (COMECs) from Stevens-Johnson syndrome (SJS) subjects with those from non-SJS subjects. Human oral mucosal epithelial cells from SJS and non-SJS subjects were cultured, and colony-forming efficiency (CFE), prolife...