Single-domain antibodies targeting neuraminidase protect against an H5N1 influenza virus challenge

• Published in: Journal of virology Vol. 88; no. 15; pp. 8278 - 8296
• Main Authors: Cardoso, Francisco Miguel, Ibañez, Lorena Itatí, Van den Hoecke, Silvie, De Baets, Sarah, Smet, Anouk, Roose, Kenny, Schepens, Bert, Descamps, Francis J, Fiers, Walter, Muyldermans, Serge, Depicker, Ann, Saelens, Xavier
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.2014
• Subjects: Animals; Antiviral Agents - immunology; Antiviral Agents - therapeutic use; Disease Models, Animal; Female; Influenza A Virus, H5N1 Subtype - drug effects; Influenza A Virus, H5N1 Subtype - immunology; Influenza virus; Inhibitory Concentration 50; Mice; Mice, Inbred BALB C; Microbial Sensitivity Tests; Neuraminidase - antagonists & inhibitors; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - prevention & control; Orthomyxoviridae Infections - virology; Single-Domain Antibodies - immunology; Single-Domain Antibodies - therapeutic use; Treatment Outcome; Vaccines and Antiviral Agents

Influenza virus neuraminidase (NA) is an interesting target of small-molecule antiviral drugs. We isolated a set of H5N1 NA-specific single-domain antibodies (N1-VHHm) and evaluated their in vitro and in vivo antiviral potential. Two of them inhibited the NA activity and in vitro replication of clad...