Mechanism of Phagolysosome Biogenesis Block by Viable Mycobacterium tuberculosis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 102; no. 11; pp. 4033 - 4038
• Main Authors: Vergne, Isabelle, Chua, Jennifer, Lee, Hwang-Ho, Lucas, Megan, Belisle, John, Deretic, Vojo, Collier, R. John
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 15.03.2005; National Acad Sciences
• Subjects: Animals; Biological Sciences; Calcium - metabolism; Cellular biology; Endosomes; Fluorescence; Lipids; Macrophages; Macrophages - microbiology; Macrophages - physiology; Mice; Microscopy; Molybdates; Mycobacterium tuberculosis; Mycobacterium tuberculosis - pathogenicity; Mycobacterium tuberculosis - physiology; Phagosomes; Phagosomes - physiology; Phosphatases; Phosphatidylinositol 3-Kinases - metabolism; Phosphatidylinositol Phosphates - metabolism; Phosphatidylinositols; Scientific imaging; Tuberculosis

Live Mycobacterium tuberculosis persists in macrophage phagosomes by interfering with phagolysosome biogenesis. Here, using four-dimensional microscopy and in vitro assays, we report the principal difference between phagosomes containing live and dead mycobacteria. Phosphatidylinositol 3-phosphate (...