Establishment and characterization of transformed goat primary cells by expression of simian virus 40 large T antigen for orf virus propagations

• Published in: PloS one Vol. 14; no. 12; p. e0226105
• Main Authors: Yamada, Yumiko, Liao, Guan-Ru, Tseng, Ching-Yu, Tseng, Yeu-Yang, Hsu, Wei-Li
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.12.2019; Public Library of Science (PLoS)
• Subjects: Animals; Antigen T (large); Antigens; Antigens, Viral, Tumor - genetics; Biology and life sciences; Cell culture; Cell Line; Cell Transformation, Viral - genetics; Dermatitis; Fibroblasts; Gene Expression; Goats; Growth kinetics; Homogeneity; Host range; Humans; Infections; Life span; Medicine and Health Sciences; Mortality; Orf; Orf virus - physiology; Pathogenesis; Physical Sciences; Plaques; Polymerase chain reaction; Propagation; Public health; Reaction kinetics; Research and Analysis Methods; Senescence; Sheep; Simian virus 40 - immunology; Smallpox; Telomerase; Vaccine development; Vaccines; Virus Replication - genetics; Viruses; Taiwan

Due to the limited host range of orf virus (ORFV), primary cells derived from its natural hosts, such as goats and sheep, are recommended for isolation and propagation of wild type ORFV. This situation limits the option for the study of virus-host interaction during ORFV infection since primary cell...