Phenotypic and Functional Characterization of Lymphocytes from Different Age Groups of Bolivian Squirrel Monkeys (Saimiri boliviensis boliviensis)

• Published in: PloS one Vol. 8; no. 11; p. e79836
• Main Authors: Nehete, Pramod N., Hanley, Patrick W., Nehete, Bharti P., Yang, Guojun, Ruiz, Julio C., Williams, Lawrence, Abee, Christian R., Sastry, K. Jagannadha
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.11.2013; Public Library of Science (PLoS)
• Subjects: Adaptive Immunity; Age; Age Factors; Aging; Analogies; Animal models; Animals; Animals, Laboratory - immunology; Antigens, CD20 - metabolism; Bolivia; Bone marrow; Cancer; CD20 antigen; CD3 antigen; CD4 antigen; CD8 antigen; Creutzfeldt-Jakob disease; Cytokines; Cytokines - blood; Cytomegalovirus; Effector cells; Enzyme-Linked Immunospot Assay; Female; Females; Gerontology; Hematology; Immune response; Immune system; Immunity, Innate; Immunological memory; Immunology; Infectious diseases; Inflammation; Interferon; Interferon-gamma - metabolism; Interleukin 10; Interleukin 12; Interleukin 6; Laboratory animals; Leukocytes (mononuclear); Lymphocytes; Lymphocytes - cytology; Lymphocytes - physiology; Lymphocytes B; Lymphocytes T; Malaria; Medical research; Memory cells; Mitogens; Monkeys; Monkeys & apes; Nutrition research; Peripheral blood mononuclear cells; Phenotype; Plasma levels; Saimiri; Saimiri - immunology; Saimiri - physiology; Saimiri boliviensis; Transmissible spongiform encephalopathy; Tumor necrosis factor-α; Vector-borne diseases; γ-Interferon; Bolivia; United States > US; Texas
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0079836

Due to many physiological and genetic characteristic similarities to humans, squirrel monkeys provide an ideal animal model specifically for studying malaria, and transmissible spongiform encephalopathies (Creutzfeldt-Jacob disease). While squirrel monkeys three years and older are generally considered adult subjects suitable for use in medical research studies, little is known about the functional properties of lymphocytes in relation to the age of these animals, which could significantly impact the quality and quantity of innate and adaptive immune responses. In this study, we investigated differences in the phenotype and function of lymphocytes subsets of young (3-4 years), adult (8-10 years) and aged (16-19 years) squirrel monkeys. In general, animals in all three age groups exhibited comparable numbers of different lymphocyte subsets except for CD20+ B cells that were significantly lower in aged relative to young animals and T cells subsets expressing both CD4 and CD8 (double positive) were significantly higher in aged relative to young animals. With increasing age, phenotypic differences in central and effector memory T cells subsets were observed, that were more pronounced for the CD8+ T cells. Despite equal proportions of CD3+ T cells among the three age groups, responses of peripheral blood mononuclear cells to T cell mitogens PHA and Con A showed lower IFN-γ producing cells in the aged group than that in the young group. Furthermore, aged animals showed significantly higher plasma levels of inflammatory cytokines IL-6, IFN-γ, TNF-α, IL-10 and IL-12. These findings suggest that while the squirrel monkeys in general share phenotypic and functional similarities of lymphocyte subsets with humans in relation to age, specific differences exist in immune function of lymphocytes between young and old animals that could potentially impact experimental outcomes for which the measurement of immunologic endpoints are critical.