Enhancing Gonococcal Antimicrobial Resistance Surveillance: a Real-Time PCR Assay for Detection of Penicillinase-Producing Neisseria gonorrhoeae by Use of Noncultured Clinical Samples

• Published in: Journal of Clinical Microbiology Vol. 49; no. 2; pp. 513 - 518
• Main Authors: Goire, Namraj, Freeman, Kevin, Tapsall, John W, Lambert, Stephen B, Nissen, Michael D, Sloots, Theo P, Whiley, David M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.2011
• Subjects: Bacteriology; Biological and medical sciences; Conserved Sequence; DNA Primers - genetics; DNA, Bacterial - genetics; Drug Resistance, Bacterial; Fundamental and applied biological sciences. Psychology; Gonorrhea - microbiology; Humans; Microbial Sensitivity Tests - methods; Microbiology; Miscellaneous; Neisseria gonorrhoeae; Neisseria gonorrhoeae - drug effects; Neisseria gonorrhoeae - enzymology; Neisseria gonorrhoeae - genetics; Neisseria gonorrhoeae - isolation & purification; Penicillinase - biosynthesis; Penicillinase - genetics; Plasmids; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Polymerase chain reaction; Resistance; Neisseria gonorrhoeae; Neisseriaceae; Bacteria; Micrococcales; Detection; Real time; Antimicrobial agent

With increasing concerns regarding diminishing treatment options for gonorrhea, maintaining the efficacy of currently used treatments and ensuring optimal Neisseria gonorrhoeae antimicrobial resistance surveillance are of the utmost importance. Penicillin is still used to treat gonorrhea in some par...