Prolonged Exposure of Primary Human Muscle Cells to Plasma Fatty Acids Associated with Obese Phenotype Induces Persistent Suppression of Muscle Mitochondrial ATP Synthase β Subunit

• Published in: PloS one Vol. 11; no. 8; p. e0160057
• Main Authors: Tran, Lee, Hanavan, Paul D., Campbell, Latoya E., De Filippis, Elena, Lake, Douglas F., Coletta, Dawn K., Roust, Lori R., Mandarino, Lawrence J., Carroll, Chad C., Katsanos, Christos S.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.08.2016; Public Library of Science (PLoS)
• Subjects: Adenosine triphosphatase; Adult; ATP; ATP synthase; Biology; Biology and Life Sciences; Body mass; Catalysis; Cell culture; Cells, Cultured; Diabetes; Dietary Fats - administration & dosage; DNA methylation; Efficiency; Epigenesis, Genetic; Esterification; Exposure; F1-ATPase; Fatty acids; Fatty Acids, Nonesterified - blood; Fatty Acids, Nonesterified - metabolism; Female; Gene expression; Glucose; Humans; Inhibitors; Insulin resistance; Life sciences; Lipids; Male; Medicine and Health Sciences; Metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Mitochondria; Mitochondria, Muscle - enzymology; Mitochondrial Proton-Translocating ATPases - genetics; Mitochondrial Proton-Translocating ATPases - metabolism; Muscle Development - genetics; Muscle Fibers, Skeletal - enzymology; Muscle Fibers, Skeletal - metabolism; Muscle Proteins - biosynthesis; Muscle Proteins - genetics; Muscles; Musculoskeletal system; MyoD Protein - genetics; Myogenin - genetics; Myotubes; Nucleic acids; Obesity; Obesity - blood; Obesity - genetics; Obesity - metabolism; Physiology; Plasma; Protein biosynthesis; Protein metabolism; Protein synthesis; Protein turnover; Proteins; Research and Analysis Methods; Rodents; Skeletal muscle; Thinness - blood; Thinness - genetics; Thinness - metabolism; Translation; Weight control; United States > US; Arizona

Our previous studies show reduced abundance of the β-subunit of mitochondrial H+-ATP synthase (β-F1-ATPase) in skeletal muscle of obese individuals. The β-F1-ATPase forms the catalytic core of the ATP synthase, and it is critical for ATP production in muscle. The mechanism(s) impairing β-F1-ATPase m...