eEF-2 Phosphorylation Down-Regulates P-Glycoprotein Over-Expression in Rat Brain Microvessel Endothelial Cells

• Published in: PloS one Vol. 10; no. 5; p. e0125389
• Main Authors: Tang, Xing Hua, Wu, Xun Yi, Xu, Lan, Fang, You Xin, Wang, Ping, Zhu, Guo Xing, Hong, Zhen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.05.2015; Public Library of Science (PLoS)
• Subjects: Animals; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Blood-brain barrier; Brain; Brain - blood supply; Brain cancer; Cell culture; Cells, Cultured; Down-Regulation; Elongation; Elongation Factor 2 Kinase - antagonists & inhibitors; Elongation Factor 2 Kinase - metabolism; Endothelial cells; Endothelial Cells - metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Gene expression; Glutamic acid; Glutamic acid receptors (ionotropic); Glycoproteins; Immunohistochemistry; Inhibitors; Kinases; Laboratory animals; Meninges; Microvessels - cytology; Multidrug resistance; N-Methyl-D-aspartic acid receptors; Neurology; Overexpression; P-Glycoprotein; Peptides; Phosphorylation; Polymerase chain reaction; Prostate cancer; Protein synthesis; Proteins; Rats; Rats, Wistar; Receptors; Rodents; Studies

We investigated whether glutamate, NMDA receptors, and eukaryote elongation factor-2 kinase (eEF-2K)/eEF-2 regulate P-glycoprotein expression, and the effects of the eEF-2K inhibitor NH125 on the expression of P-glycoprotein in rat brain microvessel endothelial cells (RBMECs). Cortex was obtained fr...