Silencing Leaf Sorbitol Synthesis Alters Long-Distance Partitioning and Apple Fruit Quality
Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (Malus domestica Borkh. cv. "Greensleeves") that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to defin...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 103; no. 49; pp. 18842 - 18847 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
05.12.2006
National Acad Sciences |
Series | Colloquium Paper |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (Malus domestica Borkh. cv. "Greensleeves") that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to define the role of sorbitol distribution in fruit development. Transgenic plants with suppressed sorbitol-6phosphate dehydrogenase compensated by accumulating sucrose and starch in leaves, and morning and midday net carbon assimilation rates were significantly lower. The sorbitol to sucrose ratio in leaves was reduced by ≈90% and in phloem exudates by ≈75%. The fruit accumulated more glucose and less fructose, starch, and malic acid, with no overall differences in weight and firmness. Sorbitol dehydrogenase activity was reduced in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffected. Analyses of transcript levels and activity of enzymes involved in carbohydrate metabolism throughout fruit development revealed significant differences in pathways related to sorbitol transport and breakdown. Together, these results suggest that sorbitol distribution plays a key role in fruit carbon metabolism and affects quality attributes such as sugar-acid balance and starch accumulation. |
---|---|
AbstractList | Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (
Malus domestica
Borkh. cv. “Greensleeves”) that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to define the role of sorbitol distribution in fruit development. Transgenic plants with suppressed sorbitol-6-phosphate dehydrogenase compensated by accumulating sucrose and starch in leaves, and morning and midday net carbon assimilation rates were significantly lower. The sorbitol to sucrose ratio in leaves was reduced by ≈90% and in phloem exudates by ≈75%. The fruit accumulated more glucose and less fructose, starch, and malic acid, with no overall differences in weight and firmness. Sorbitol dehydrogenase activity was reduced in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffected. Analyses of transcript levels and activity of enzymes involved in carbohydrate metabolism throughout fruit development revealed significant differences in pathways related to sorbitol transport and breakdown. Together, these results suggest that sorbitol distribution plays a key role in fruit carbon metabolism and affects quality attributes such as sugar–acid balance and starch accumulation. Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (Malus domestica Borkh. cv. "Greensleeves") that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to define the role of sorbitol distribution in fruit development. Transgenic plants with suppressed sorbitol-6phosphate dehydrogenase compensated by accumulating sucrose and starch in leaves, and morning and midday net carbon assimilation rates were significantly lower. The sorbitol to sucrose ratio in leaves was reduced by ≈90% and in phloem exudates by ≈75%. The fruit accumulated more glucose and less fructose, starch, and malic acid, with no overall differences in weight and firmness. Sorbitol dehydrogenase activity was reduced in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffected. Analyses of transcript levels and activity of enzymes involved in carbohydrate metabolism throughout fruit development revealed significant differences in pathways related to sorbitol transport and breakdown. Together, these results suggest that sorbitol distribution plays a key role in fruit carbon metabolism and affects quality attributes such as sugar-acid balance and starch accumulation. Sorbitol and sucrose are major products of photosynthesis distributed in apple trees ( Malus domestica Borkh. cv. “Greensleeves”) that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to define the role of sorbitol distribution in fruit development. Transgenic plants with suppressed sorbitol-6-phosphate dehydrogenase compensated by accumulating sucrose and starch in leaves, and morning and midday net carbon assimilation rates were significantly lower. The sorbitol to sucrose ratio in leaves was reduced by ≈90% and in phloem exudates by ≈75%. The fruit accumulated more glucose and less fructose, starch, and malic acid, with no overall differences in weight and firmness. Sorbitol dehydrogenase activity was reduced in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffected. Analyses of transcript levels and activity of enzymes involved in carbohydrate metabolism throughout fruit development revealed significant differences in pathways related to sorbitol transport and breakdown. Together, these results suggest that sorbitol distribution plays a key role in fruit carbon metabolism and affects quality attributes such as sugar–acid balance and starch accumulation. gene silencing sugar–acid balance translocation starch accumulation Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (Malus domestica Borkh. cv. "Greensleeves") that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to define the role of sorbitol distribution in fruit development. Transgenic plants with suppressed sorbitol-6-phosphate dehydrogenase compensated by accumulating sucrose and starch in leaves, and morning and midday net carbon assimilation rates were significantly lower. The sorbitol to sucrose ratio in leaves was reduced by approximately 90% and in phloem exudates by approximately 75%. The fruit accumulated more glucose and less fructose, starch, and malic acid, with no overall differences in weight and firmness. Sorbitol dehydrogenase activity was reduced in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffected. Analyses of transcript levels and activity of enzymes involved in carbohydrate metabolism throughout fruit development revealed significant differences in pathways related to sorbitol transport and breakdown. Together, these results suggest that sorbitol distribution plays a key role in fruit carbon metabolism and affects quality attributes such as sugar-acid balance and starch accumulation. Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (Malus domestica Borkh. cv. "Greensleeves") that affect quality in fruit. Transgenic apple plants were silenced or up-regulated for sorbitol-6-phosphate dehydrogenase by using the CaMV35S promoter to define the role of sorbitol distribution in fruit development. Transgenic plants with suppressed sorbitol-6-phosphate dehydrogenase compensated by accumulating sucrose and starch in leaves, and morning and midday net carbon assimilation rates were significantly lower. The sorbitol to sucrose ratio in leaves was reduced by [asymptotically =]90% and in phloem exudates by [asymptotically =]75%. The fruit accumulated more glucose and less fructose, starch, and malic acid, with no overall differences in weight and firmness. Sorbitol dehydrogenase activity was reduced in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffected. Analyses of transcript levels and activity of enzymes involved in carbohydrate metabolism throughout fruit development revealed significant differences in pathways related to sorbitol transport and breakdown. Together, these results suggest that sorbitol distribution plays a key role in fruit carbon metabolism and affects quality attributes such as sugar-acid balance and starch accumulation. [PUBLICATION ABSTRACT] |
Author | Ormonde, Nichole DeJong, Ted M. Teo, Gianni Suzuki, Yasuo Dandekar, Abhaya M. Uratsu, Sandie L. Lampinen, Bruce Hu, William K. |
Author_xml | – sequence: 1 givenname: Gianni surname: Teo fullname: Teo, Gianni – sequence: 2 givenname: Yasuo surname: Suzuki fullname: Suzuki, Yasuo – sequence: 3 givenname: Sandie L. surname: Uratsu fullname: Uratsu, Sandie L. – sequence: 4 givenname: Bruce surname: Lampinen fullname: Lampinen, Bruce – sequence: 5 givenname: Nichole surname: Ormonde fullname: Ormonde, Nichole – sequence: 6 givenname: William K. surname: Hu fullname: Hu, William K. – sequence: 7 givenname: Ted M. surname: DeJong fullname: DeJong, Ted M. – sequence: 8 givenname: Abhaya M. surname: Dandekar fullname: Dandekar, Abhaya M. |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/17132742$$D View this record in MEDLINE/PubMed |
BookMark | eNqF0r9vEzEUB3ALFdG0MDOBTgxIDNc-_7jzeUGKCgWkSIACE4Pl8_lSR44dbB8i_z2OEjXA0smDP-_pPX99gc588Aah5xiuMHB6vfUqXUELTccpBvoIzTAIXLdMwBmaARBed4ywc3SR0hoARNPBE3SOOaaEMzJDP5bWGa-tX1ULo8ZqGWJvc3DVcufznUk2VXOXTUzVIvhV_c6mrLw21RcVs802-H2l8kM1326dqW7jZHP1dVLO5t1T9HhULplnx_MSfb99_-3mY734_OHTzXxR64a0ucac8VEPui9Dc92wlgoCuhsUUNGrHjqFOzr0mioMI9UaBkaZ4ZoLYEJrQy_R20Pf7dRvzKCNz1E5uY12o-JOBmXlvzfe3slV-CVxKyhnojR4fWwQw8_JpCw3NmnjnPImTEm2HSFtg-mDEIsGAzRtga_-g-swRV9eQRLApMOYk4KuD0jHkFI04_3IGOQ-XrmPV57iLRUv_9705I95FvDmCPaVp3ZUMiFxV_6CHCdXAv2di60esIW8OJB1yiHeG1p2xASA_gF0F8WV |
CitedBy_id | crossref_primary_10_1186_1471_2164_11_2 crossref_primary_10_1134_S1022795417040032 crossref_primary_10_1134_S1022795415110046 crossref_primary_10_3389_fpls_2019_01233 crossref_primary_10_1016_j_foodchem_2010_05_053 crossref_primary_10_1093_pcp_pcaa068 crossref_primary_10_1016_j_plantsci_2020_110787 crossref_primary_10_1016_j_postharvbio_2018_01_012 crossref_primary_10_17660_ActaHortic_2019_1260_1 crossref_primary_10_1007_s13562_018_0459_5 crossref_primary_10_7868_S0016675817040038 crossref_primary_10_1007_s11103_008_9362_0 crossref_primary_10_1016_j_plantsci_2010_01_010 crossref_primary_10_1111_jipb_12041 crossref_primary_10_1111_ppl_12106 crossref_primary_10_1016_j_plaphy_2022_12_003 crossref_primary_10_1111_tpj_13774 crossref_primary_10_21273_JASHS_138_6_443 crossref_primary_10_1038_s41438_018_0064_8 crossref_primary_10_21273_JASHS_135_4_379 crossref_primary_10_1016_j_molliq_2019_02_117 crossref_primary_10_1007_s00217_012_1896_3 crossref_primary_10_17660_ActaHortic_2018_1206_11 crossref_primary_10_3390_agronomy11071368 crossref_primary_10_1002_jsfa_7386 crossref_primary_10_1021_acschembio_8b00804 crossref_primary_10_5897_AJB2021_17348 crossref_primary_10_1016_j_envexpbot_2020_104031 crossref_primary_10_1111_j_1399_3054_2008_01068_x crossref_primary_10_1007_s10535_015_0581_3 crossref_primary_10_1002_poc_4146 crossref_primary_10_1016_j_envexpbot_2018_04_015 crossref_primary_10_17660_eJHS_2022_033 crossref_primary_10_1016_S2095_3119_20_63593_6 crossref_primary_10_1093_treephys_tpab154 crossref_primary_10_1093_jxb_ern347 crossref_primary_10_17660_ActaHortic_2017_1160_42 crossref_primary_10_1016_j_gene_2019_06_022 crossref_primary_10_1016_j_plaphy_2008_10_009 crossref_primary_10_1186_s12864_020_07299_y crossref_primary_10_1016_j_postharvbio_2023_112362 crossref_primary_10_1007_s12230_018_9640_6 crossref_primary_10_1093_mp_ssr051 crossref_primary_10_2503_jjshs1_79_1 crossref_primary_10_3390_ijms242316992 crossref_primary_10_3390_ijms22063066 crossref_primary_10_1111_nph_14824 crossref_primary_10_1016_j_phytochem_2014_04_008 crossref_primary_10_1002_jsfa_10005 crossref_primary_10_1016_j_foodchem_2012_11_024 crossref_primary_10_1007_s11105_011_0409_z crossref_primary_10_1016_j_plantsci_2013_04_008 crossref_primary_10_1016_j_plgene_2021_100312 crossref_primary_10_1016_j_postharvbio_2012_05_007 crossref_primary_10_1016_j_algal_2019_101520 crossref_primary_10_1590_S0103_90162007000600014 crossref_primary_10_1021_jf403064p crossref_primary_10_1038_hortres_2015_47 crossref_primary_10_3390_agronomy10121912 crossref_primary_10_1007_s00425_014_2076_4 crossref_primary_10_1016_j_plantsci_2014_11_002 crossref_primary_10_7235_hort_2015_14179 crossref_primary_10_1016_j_biochi_2009_09_013 crossref_primary_10_3389_fpls_2019_01248 crossref_primary_10_1007_s11103_017_0669_6 crossref_primary_10_1371_journal_pone_0187235 crossref_primary_10_3390_ijms160613065 crossref_primary_10_1002_jib_338 crossref_primary_10_1093_jxb_ery118 crossref_primary_10_3389_fpls_2015_00208 crossref_primary_10_1021_jf404109t crossref_primary_10_1007_s10725_021_00765_x crossref_primary_10_1111_pbi_12002 crossref_primary_10_1016_j_scienta_2016_08_027 crossref_primary_10_1111_tpj_15624 crossref_primary_10_1039_C9RA02138H crossref_primary_10_1016_j_jiec_2018_02_004 crossref_primary_10_1134_S1022795414120023 crossref_primary_10_7235_HORT_20210017 crossref_primary_10_1021_acs_jafc_2c05102 crossref_primary_10_2503_jjshs1_78_158 crossref_primary_10_1016_j_jprot_2013_02_006 crossref_primary_10_3390_agronomy12112877 crossref_primary_10_3390_foods12071425 crossref_primary_10_1111_pce_13576 crossref_primary_10_1038_s41438_018_0099_x crossref_primary_10_1007_s10725_017_0294_z crossref_primary_10_3390_horticulturae8070649 crossref_primary_10_1021_acs_jafc_2c01102 crossref_primary_10_1371_journal_pone_0022247 crossref_primary_10_1016_j_scienta_2018_09_019 crossref_primary_10_1016_j_phytochem_2017_09_019 crossref_primary_10_1016_j_plaphy_2023_107955 crossref_primary_10_1093_jxb_erx391 crossref_primary_10_1071_FP11105 |
Cites_doi | 10.1007/s00425-003-0975-x 10.1104/pp.114.1.307 10.21273/JASHS.130.2.261 10.1023/A:1003769304778 10.1002/j.1460-2075.1994.tb06229.x 10.1093/nar/17.20.8385 10.1104/pp.67.1.139 10.21273/JASHS.117.4.607 10.1104/pp.113.4.1379 10.1105/tpc.13.3.695 10.1002/j.1460-2075.1987.tb02730.x 10.1007/978-94-011-1584-1_11 10.1016/j.pbi.2004.03.014 10.1104/pp.78.1.149 10.1093/jexbot/52.356.551 10.1146/annurev.arplant.57.032905.105441 10.1073/pnas.90.13.6160 10.1016/0003-2697(76)90527-3 10.1093/oxfordjournals.pcp.a078789 10.1007/s00425-004-1384-5 10.1016/S0165-2427(99)00100-2 10.1104/pp.118.1.59 10.21273/JASHS.111.1.134 10.1073/pnas.76.9.4350 10.1111/j.1399-3054.2004.00344.x 10.1007/BF00272054 10.1016/j.plantsci.2004.02.024 10.1006/abio.1994.1538 10.1034/j.1399-3054.1999.105301.x 10.17660/ActaHortic.1998.466.3 10.2503/jjshs.69.671 10.1038/227680a0 10.1016/S0176-1617(97)80252-0 10.21273/JASHS.129.6.0870 10.1104/pp.100.3.1607 10.1016/S0176-1617(97)80253-2 10.1016/S0021-9258(18)75382-9 10.1146/annurev.arplant.48.1.191 10.1021/jf9701704 10.1104/pp.103.036632 10.1104/pp.102.016725 |
ContentType | Journal Article |
Copyright | Copyright 2006 National Academy of Sciences of the United States of America Copyright National Academy of Sciences Dec 5, 2006 2006 by The National Academy of Sciences of the USA 2006 |
Copyright_xml | – notice: Copyright 2006 National Academy of Sciences of the United States of America – notice: Copyright National Academy of Sciences Dec 5, 2006 – notice: 2006 by The National Academy of Sciences of the USA 2006 |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7QO 7X8 5PM |
DOI | 10.1073/pnas.0605873103 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts Biotechnology Research Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Nucleic Acids Abstracts Ecology Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Entomology Abstracts Genetics Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Immunology Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Biotechnology Research Abstracts MEDLINE - Academic |
DatabaseTitleList | CrossRef Genetics Abstracts MEDLINE Virology and AIDS Abstracts MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) |
EISSN | 1091-6490 |
EndPage | 18847 |
ExternalDocumentID | 1176654101 10_1073_pnas_0605873103 17132742 103_49_18842 30051200 |
Genre | Validation Study Research Support, Non-U.S. Gov't Journal Article Feature |
GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 123 29P 2AX 2FS 2WC 3O- 4.4 53G 5RE 5VS 79B 85S AACGO AAFWJ AANCE AAYJJ ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACIWK ACNCT ACPRK ADULT ADZLD AENEX AEUPB AEXZC AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM ASUFR AS~ BKOMP CS3 D0L DCCCD DIK DNJUQ DOOOF DU5 DWIUU E3Z EBS EJD F20 F5P FRP GX1 HH5 HQ3 HTVGU HYE JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST KQ8 L7B LU7 MVM N9A N~3 O9- OK1 P-O PNE PQQKQ R.V RHF RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR VQA W8F WH7 WHG WOQ WOW X7M XFK XSW Y6R YBH YKV YSK ZA5 ZCA ZCG ~02 ~KM - 02 0R 1AW 55 AAPBV ABFLS ABPTK ADACO AJYGW AS DZ GJ KM PQEST X XHC ADACV CGR CUY CVF ECM EIF H13 IPSME NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7QO 7X8 5PM |
ID | FETCH-LOGICAL-c526t-1747fcdcb4907c5463920c8da039bab08a183dbc3a10f3cc0d434e7c79049cce3 |
IEDL.DBID | RPM |
ISSN | 0027-8424 |
IngestDate | Tue Sep 17 21:20:48 EDT 2024 Sat Aug 17 05:48:34 EDT 2024 Sat Aug 17 02:45:03 EDT 2024 Thu Oct 10 16:14:52 EDT 2024 Fri Aug 23 01:50:56 EDT 2024 Fri Oct 18 09:15:42 EDT 2024 Thu May 30 08:51:21 EDT 2019 Wed Nov 11 00:29:19 EST 2020 Fri Feb 02 07:05:37 EST 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 49 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c526t-1747fcdcb4907c5463920c8da039bab08a183dbc3a10f3cc0d434e7c79049cce3 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 ObjectType-Undefined-3 Author contributions: G.T. and Y.S. contributed equally to this work; T.M.D. and A.M.D. designed research; G.T., Y.S., S.L.U., B.L., N.O., and W.K.H. performed research; Y.S. contributed new reagents/analytic tools; G.T., B.L., and T.M.D. analyzed data; and G.T., Y.S., and A.M.D. wrote the paper. Edited by Diter von Wettstein, Washington State University, Pullman, WA, and approved October 18, 2006 |
OpenAccessLink | https://doi.org/10.1073/pnas.0605873103 |
PMID | 17132742 |
PQID | 201281172 |
PQPubID | 42026 |
PageCount | 6 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_1693749 proquest_miscellaneous_68226513 proquest_journals_201281172 jstor_primary_30051200 pnas_primary_103_49_18842 proquest_miscellaneous_19510056 pubmed_primary_17132742 pnas_primary_103_49_18842_fulltext crossref_primary_10_1073_pnas_0605873103 |
ProviderPackageCode | RNA PNE |
PublicationCentury | 2000 |
PublicationDate | 2006-12-05 |
PublicationDateYYYYMMDD | 2006-12-05 |
PublicationDate_xml | – month: 12 year: 2006 text: 2006-12-05 day: 05 |
PublicationDecade | 2000 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States – name: Washington |
PublicationSeriesTitle | Colloquium Paper |
PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
PublicationTitleAlternate | Proc Natl Acad Sci U S A |
PublicationYear | 2006 |
Publisher | National Academy of Sciences National Acad Sciences |
Publisher_xml | – name: National Academy of Sciences – name: National Acad Sciences |
References | e_1_3_4_3_2 e_1_3_4_2_2 Lo Bianco R (e_1_3_4_37_2) 2001; 36 e_1_3_4_9_2 e_1_3_4_7_2 e_1_3_4_41_2 e_1_3_4_6_2 e_1_3_4_40_2 e_1_3_4_5_2 e_1_3_4_4_2 e_1_3_4_22_2 e_1_3_4_45_2 e_1_3_4_23_2 e_1_3_4_44_2 e_1_3_4_20_2 e_1_3_4_43_2 e_1_3_4_21_2 Bieleski RL (e_1_3_4_1_2) 1985; 12 e_1_3_4_26_2 e_1_3_4_27_2 e_1_3_4_24_2 e_1_3_4_47_2 e_1_3_4_25_2 e_1_3_4_46_2 e_1_3_4_28_2 Dandekar AM (e_1_3_4_30_2) 2006 Yamaki S (e_1_3_4_42_2) 1980; 69 Klages K (e_1_3_4_33_2) 2001; 28 James DJ (e_1_3_4_29_2) 1989; 7 Kanayama Y (e_1_3_4_8_2) 1993; 34 e_1_3_4_11_2 e_1_3_4_34_2 e_1_3_4_12_2 e_1_3_4_32_2 e_1_3_4_10_2 e_1_3_4_31_2 e_1_3_4_15_2 e_1_3_4_16_2 e_1_3_4_13_2 e_1_3_4_36_2 e_1_3_4_14_2 e_1_3_4_35_2 e_1_3_4_19_2 e_1_3_4_17_2 Yamaki S (e_1_3_4_38_2) 1986; 111 e_1_3_4_18_2 e_1_3_4_39_2 |
References_xml | – ident: e_1_3_4_15_2 doi: 10.1007/s00425-003-0975-x – ident: e_1_3_4_24_2 doi: 10.1104/pp.114.1.307 – ident: e_1_3_4_25_2 doi: 10.21273/JASHS.130.2.261 – ident: e_1_3_4_26_2 doi: 10.1023/A:1003769304778 – ident: e_1_3_4_16_2 doi: 10.1002/j.1460-2075.1994.tb06229.x – ident: e_1_3_4_28_2 doi: 10.1093/nar/17.20.8385 – ident: e_1_3_4_41_2 doi: 10.1104/pp.67.1.139 – ident: e_1_3_4_32_2 doi: 10.21273/JASHS.117.4.607 – ident: e_1_3_4_45_2 doi: 10.1104/pp.113.4.1379 – ident: e_1_3_4_4_2 doi: 10.1105/tpc.13.3.695 – volume: 36 start-page: 574 year: 2001 ident: e_1_3_4_37_2 publication-title: HortScience contributor: fullname: Lo Bianco R – ident: e_1_3_4_31_2 doi: 10.1002/j.1460-2075.1987.tb02730.x – volume: 28 start-page: 131 year: 2001 ident: e_1_3_4_33_2 publication-title: Aus J Plant Physiol contributor: fullname: Klages K – start-page: 253 volume-title: Methods in Molecular Biology year: 2006 ident: e_1_3_4_30_2 contributor: fullname: Dandekar AM – ident: e_1_3_4_19_2 doi: 10.1007/978-94-011-1584-1_11 – ident: e_1_3_4_22_2 doi: 10.1016/j.pbi.2004.03.014 – ident: e_1_3_4_44_2 doi: 10.1104/pp.78.1.149 – ident: e_1_3_4_3_2 doi: 10.1093/jexbot/52.356.551 – ident: e_1_3_4_23_2 doi: 10.1146/annurev.arplant.57.032905.105441 – ident: e_1_3_4_14_2 doi: 10.1073/pnas.90.13.6160 – ident: e_1_3_4_40_2 doi: 10.1016/0003-2697(76)90527-3 – ident: e_1_3_4_11_2 doi: 10.1093/oxfordjournals.pcp.a078789 – ident: e_1_3_4_13_2 doi: 10.1007/s00425-004-1384-5 – ident: e_1_3_4_35_2 doi: 10.1016/S0165-2427(99)00100-2 – ident: e_1_3_4_17_2 doi: 10.1104/pp.118.1.59 – volume: 12 start-page: 657 year: 1985 ident: e_1_3_4_1_2 publication-title: Aus J Plant Physiol contributor: fullname: Bieleski RL – volume: 111 start-page: 134 year: 1986 ident: e_1_3_4_38_2 publication-title: J Am Soc Hort Sci doi: 10.21273/JASHS.111.1.134 contributor: fullname: Yamaki S – ident: e_1_3_4_47_2 doi: 10.1073/pnas.76.9.4350 – ident: e_1_3_4_9_2 doi: 10.1111/j.1399-3054.2004.00344.x – volume: 7 start-page: 658 year: 1989 ident: e_1_3_4_29_2 publication-title: Plant Cell Rep doi: 10.1007/BF00272054 contributor: fullname: James DJ – ident: e_1_3_4_12_2 doi: 10.1016/j.plantsci.2004.02.024 – ident: e_1_3_4_36_2 doi: 10.1006/abio.1994.1538 – ident: e_1_3_4_21_2 doi: 10.1034/j.1399-3054.1999.105301.x – ident: e_1_3_4_18_2 doi: 10.17660/ActaHortic.1998.466.3 – ident: e_1_3_4_39_2 doi: 10.2503/jjshs.69.671 – volume: 69 start-page: 117 year: 1980 ident: e_1_3_4_42_2 publication-title: Plant Cell Physiol contributor: fullname: Yamaki S – ident: e_1_3_4_46_2 doi: 10.1038/227680a0 – ident: e_1_3_4_7_2 doi: 10.1016/S0176-1617(97)80252-0 – volume: 34 start-page: 819 year: 1993 ident: e_1_3_4_8_2 publication-title: Plant Cell Physiol contributor: fullname: Kanayama Y – ident: e_1_3_4_10_2 doi: 10.21273/JASHS.129.6.0870 – ident: e_1_3_4_27_2 doi: 10.1104/pp.100.3.1607 – ident: e_1_3_4_20_2 doi: 10.1016/S0176-1617(97)80253-2 – ident: e_1_3_4_43_2 doi: 10.1016/S0021-9258(18)75382-9 – ident: e_1_3_4_2_2 doi: 10.1146/annurev.arplant.48.1.191 – ident: e_1_3_4_34_2 doi: 10.1021/jf9701704 – ident: e_1_3_4_6_2 doi: 10.1104/pp.103.036632 – ident: e_1_3_4_5_2 doi: 10.1104/pp.102.016725 |
SSID | ssj0009580 |
Score | 2.2895114 |
Snippet | Sorbitol and sucrose are major products of photosynthesis distributed in apple trees (Malus domestica Borkh. cv. "Greensleeves") that affect quality in fruit.... Sorbitol and sucrose are major products of photosynthesis distributed in apple trees ( Malus domestica Borkh. cv. “Greensleeves”) that affect quality in fruit.... Sorbitol and sucrose are major products of photosynthesis distributed in apple trees ( Malus domestica Borkh. cv. “Greensleeves”) that affect quality in fruit.... |
SourceID | pubmedcentral proquest crossref pubmed pnas jstor |
SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
StartPage | 18842 |
SubjectTerms | Apples Biological Sciences Dehydrogenases Enzymes Fruit - enzymology Fruit - genetics Fruit - metabolism Fruit trees Fruits Gene Silencing Kinases Leaves Malus - enzymology Malus - genetics Malus - metabolism Malus domestica Metabolism Phloem Photosynthesis Plant Leaves - enzymology Plant Leaves - genetics Plant Leaves - metabolism Plants Plants, Genetically Modified Sorbitol - antagonists & inhibitors Sorbitol - metabolism Starches Sugar Alcohol Dehydrogenases - genetics Sugars Transgenic plants |
Title | Silencing Leaf Sorbitol Synthesis Alters Long-Distance Partitioning and Apple Fruit Quality |
URI | https://www.jstor.org/stable/30051200 http://www.pnas.org/content/103/49/18842.abstract https://www.ncbi.nlm.nih.gov/pubmed/17132742 https://www.proquest.com/docview/201281172 https://search.proquest.com/docview/19510056 https://search.proquest.com/docview/68226513 https://pubmed.ncbi.nlm.nih.gov/PMC1693749 |
Volume | 103 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3BbtQwEB21PXFBtFAIpcVCHMohu07sxMkRVVQFVIQElSpxsGzHgUhb72qze-jfM3acXRbRC2ePI2s8Y7_JzDwDvG1yKmzV1KnVBUt5K1SqqWnTumGlLjLdlqVvTr7-Ul7d8E-3xe0eFGMvTCjaN7qbuNndxHW_Qm3l4s5Mxzqx6dfrC08gIng93Yd9wdgYom-Ydquh7yTH45fnfOTzEWy6cKqfUJ8IFP55LU8YijGaT1bu3EpDYaJnO0X5fyHPvwso_7iRLp_A4wglyfthyYewZ90RHEZn7cl5ZJR-9xR-fOt8bxHeUmRmVUv6-VKjJ89If-8QAPZdT0LWvCezufuZNh5T4jfIwqsj_rElyjXEp7staZfrbkWGdsz7Z3Bz-eH7xVUaX1VITZGXqxRDENGaxmiOcbHxbPh1Tk3VKMpqrTStFHp5ow1TGW2ZMbThjFthRI3BhDGWHcOBmzv7AohmhRKmqkxZ1Vzh2ZfluU8TMqVqayuewPmoVbkYyDNkSHoLJr1u5XYvEjgOWt_IeRr9DF04gSSIbuczyWuZVbi3Cbx5cEy2sXgmgZNxA2X0z17mIYOI4C2B15tRdCyfLVHOzte9zDz2RHj4sESJ4KosMlz788EctguJZpWA2DGUjYAn9d4dQVsP5N7Rtl_-98wTeJTHx5Vo8QoOVsu1PUXgtNJnGDJ8_HwW3OU3h-YYtg |
link.rule.ids | 230,315,733,786,790,891,27955,27956,53825,53827 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB2VcoALpUBLKFALcSiHZJPYiZMjqqgW6FZItKgSB8t2HIjYeleb7KH99YzzsctW9ABnTxJbMx6_ycw8A7wt4pCbrMh9oxLqs5JLX4W69POCpiqJVJmmrjl5cpaOL9iny-RyC5KhF6Yt2teqCuz0KrDVz7a2cn6lR0Od2OjL5NgRiHCWj-7BfdyvcTIE6Suu3azrPInRAbOYDYw-nI7mVtZB6FKB3F2w5ShDMUpz6cqNc6krTXR8pyj_N-x5u4TyjzPpZAe-DavpSlF-BctGBfrmFtHjPy_3MTzqUSp53w3vwpaxT2C39wM1OerJqt89he9fK9e2hAcgmRpZknq2UOgkpqS-togt66ombUK-JtOZ_eEXDq7iO8jcfbr_GUykLYjLpBtSLpZVQ7pOz-tncHHy4fx47PcXNvg6idPGx-iGl7rQimHIrR3Rfh6HOitkSHMlVZhJdCCF0lRGYUm1DgtGmeGa5xinaG3oHmzbmTXPgSiaSK6zTKdZziS61SiOXQaSSpkbkzEPjgZ1iXnHyyHafDqnwilNrJXswV6rzpWcY-iP0Dt44LWi6-epYLmIMjQaD97cOSbKvi7Hg4PBMkS_9WsRt8lJxIUeHK5Gcc-6RIy0ZrasReRgLSLPuyVSxG1pEuHc9zs7W0-kt1cP-IYFrgQcX_jmCNpVyxve29GL_37yEB6Mzyen4vTj2ecDeBj3dziFyUvYbhZL8wrxWaNet7vxN_9qObo |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BkRAXoEBLKFALcSiHbB524uSICqvyaFUJKlVwsGzHgYitN9pkD-XXM85jt1vRS8-eJLZmPP4mM_MZ4G0Rh9xkRe4blVCflVz6KtSlnxc0VUmkyjR1zcnHJ-nRGft8npxfueqrK9rXqprY2cXEVr-72sr6QgdjnVhwenzoCEQ4y4O6KIO7cA_3bMzHQH3Ft5v13ScxOmEWs5HVh9OgtrKZhC4dyN0lW442FCM1l7LcOJv68kTHeYry_8Of18sor5xL00fwY1xRX47yZ7Js1UT_vUb2eKslP4aHA1ol73uRbbhj7BPYHvxBQw4G0up3T-Hnt8q1L-FBSGZGlqSZLxQ6ixlpLi1izKZqSJeYb8hsbn_5hYOt-A5Su88PP4WJtAVxGXVDysWyaknf8Xn5DM6mH78fHvnDxQ2-TuK09THK4aUutGIYemtHuJ_Hoc4KGdJcSRVmEh1JoTSVUVhSrcOCUWa45jnGK1obugNbdm7NcyCKJpLrLNNpljOJ7jWKY5eJpFLmxmTMg4NRZaLu-TlEl1fnVDjFibWiPdjpVLqSc0z9EXoJD7xOdP08FSwXUYaG48GbG8dEOdTneLA3WocYXEAj4i5JifjQg_3VKO5dl5CR1syXjYgcvEUEerNEivgtTSKc-25va-uJDDbrAd-wwpWA4w3fHEHb6vjDB1t6cesn9-H-6Yep-Prp5MsePIiHq5zC5CVstYuleYUwrVWvuw35D8cOPDo |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Silencing+Leaf+Sorbitol+Synthesis+Alters+Long-Distance+Partitioning+and+Apple+Fruit+Quality&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Teo%2C+Gianni&rft.au=Suzuki%2C+Yasuo&rft.au=Uratsu%2C+Sandie+L.&rft.au=Lampinen%2C+Bruce&rft.date=2006-12-05&rft.pub=National+Academy+of+Sciences&rft.issn=0027-8424&rft.eissn=1091-6490&rft.volume=103&rft.issue=49&rft.spage=18842&rft.epage=18847&rft_id=info:doi/10.1073%2Fpnas.0605873103&rft.externalDocID=30051200 |
thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F103%2F49.cover.gif |
thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F103%2F49.cover.gif |