Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes
Abstract Background aims The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods Human adult stem cells from lipoaspirates were transiently permeabilized, expo...
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| Published in | Cytotherapy (Oxford, England) Vol. 12; no. 3; pp. 332 - 337 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Inc
2010
Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS) |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Abstract Background aims The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. Results After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric α-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription–polymerase chain reaction (cardiac myosin light chain 1, α-cardiac actin, cardiac troponin T and cardiac β-myosin). Conclusions We have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease. |
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| AbstractList | The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue.
Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture.
After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric alpha-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription-polymerase chain reaction (cardiac myosin light chain 1, alpha-cardiac actin, cardiac troponin T and cardiac beta-myosin).
We have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease. Abstract Background aims The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. Results After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric α-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription–polymerase chain reaction (cardiac myosin light chain 1, α-cardiac actin, cardiac troponin T and cardiac β-myosin). Conclusions We have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease. The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric α-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription–polymerase chain reaction (cardiac myosin light chain 1, α-cardiac actin, cardiac troponin T and cardiac β-myosin). We have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease. BACKGROUND AIMSThe goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue.METHODSHuman adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture.RESULTSAfter 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric alpha-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription-polymerase chain reaction (cardiac myosin light chain 1, alpha-cardiac actin, cardiac troponin T and cardiac beta-myosin).CONCLUSIONSWe have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease. Abstract Background aims. The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods. Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. Results. After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric α-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription-polymerase chain reaction (cardiac myosin light chain 1, α-cardiac actin, cardiac troponin T and cardiac β-myosin). Conclusions. We have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease. |
| Author | López, Elena Boulaiz, Houria Jiménez-Navarro, Manuel Carrillo, Esmeralda Perán, Macarena Marchal, Juan A. Tosh, David Rodríguez-Serrano, Fernando Sánchez-Espin, Gema Aranega, Antonia de Teresa, Eduardo |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20230311$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1073/pnas.0600635103 10.1038/nbt0502-460 10.1111/j.1745-7254.2007.00553.x 10.1161/01.RES.0000265074.83288.09 10.1007/s10517-005-0430-z 10.1093/cvr/cvn073 10.1038/nature08039 10.1016/j.bbrc.2003.12.109 10.1080/00365510701836907 10.1016/j.yjmcc.2007.07.056 10.3109/14653240902988818 10.1038/nbt1117 10.1016/j.bbrc.2008.09.076 10.1111/j.1432-0436.2007.00163.x 10.1177/153537020623100105 10.1016/S0022-5223(03)00074-6 10.1016/j.bbrc.2006.12.103 10.1172/JCI5298 |
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| References | Gaustad, Boquest, Anderson, Gerdes, Collas (bib0035) 2004; 314 Urbanek, Cesselli, Rota, Nascimbene, de Angelis, Hosoda (bib0010) 2006; 103 Miguel, Erices (bib0075) 2006; 231 Laflamme, Murry (bib0005) 2005; 23 Jin, Sun, Zhang, Wang, Ju, Lou (bib0030) 2007; 28 Romanov, Darevskaya, Merzlikina, Buravkova (bib0020) 2005; 140 Koninckx, Hensen, Daniëls, Moreels, Lambrichts, Jongen (bib0085) 2009; 11 Moore, Tsang, Rushing, Lin, Tse, Chan (bib0050) 2008; 377 Guan, Hasenfuss (bib0015) 2007; 43 Bai, Pinkernell, Song, Nabzdyk, Reiser, Alt (bib0060) 2007; 353 Gimble, Katz, Bunnell (bib0040) 2007; 100 Makino, Fukuda, Miyoshi, Konishi, Kodama, Pan (bib0045) 1999; 103 Rangappa, Entwistle, Wechsler, Kresh (bib0070) 2003; 126 Chen, Huang, Dahme, Rottbauer, Ackerman, Xu (bib0065) 2008; 79 Schimrosczyk, Song, Vykoukal, Vykoukal, Bai, Krohn (bib0080) 2008; 68 Håkelien, Landsverk, Robl, Skålhegg, Collas (bib0025) 2002; 20 Takeuchi, Bruneau (bib0090) 2009; 459 Höllrigl, Hofner, Stary, Weitzer (bib0055) 2007; 75 Laflamme (10.3109/14653240903548202_bib0005) 2005; 23 Jin (10.3109/14653240903548202_bib0030) 2007; 28 Urbanek (10.3109/14653240903548202_bib0010) 2006; 103 Guan (10.3109/14653240903548202_bib0015) 2007; 43 Rangappa (10.3109/14653240903548202_bib0070) 2003; 126 Schimrosczyk (10.3109/14653240903548202_bib0080) 2008; 68 Koninckx (10.3109/14653240903548202_bib0085) 2009; 11 Romanov (10.3109/14653240903548202_bib0020) 2005; 140 Gaustad (10.3109/14653240903548202_bib0035) 2004; 314 Moore (10.3109/14653240903548202_bib0050) 2008; 377 Höllrigl (10.3109/14653240903548202_bib0055) 2007; 75 Takeuchi (10.3109/14653240903548202_bib0090) 2009; 459 Miguel (10.3109/14653240903548202_bib0075) 2006; 231 Makino (10.3109/14653240903548202_bib0045) 1999; 103 Chen (10.3109/14653240903548202_bib0065) 2008; 79 Bai (10.3109/14653240903548202_bib0060) 2007; 353 Håkelien (10.3109/14653240903548202_bib0025) 2002; 20 Gimble (10.3109/14653240903548202_bib0040) 2007; 100 |
| References_xml | – volume: 140 start-page: 138 year: 2005 end-page: 143 ident: bib0020 article-title: Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation potentialities publication-title: Bull Exp Biol Med contributor: fullname: Buravkova – volume: 353 start-page: 665 year: 2007 end-page: 671 ident: bib0060 article-title: Genetically selected stem cells from human adipose tissue express cardiac markers publication-title: Biochem Biophys Res Commun contributor: fullname: Alt – volume: 100 start-page: 1249 year: 2007 end-page: 1260 ident: bib0040 article-title: Adipose-derived stem cells for regenerative medicine publication-title: Circ Res contributor: fullname: Bunnell – volume: 103 start-page: 9226 year: 2006 end-page: 9231 ident: bib0010 article-title: Stem cell niches in the adult mouse heart publication-title: Proc Natl Acad Sci USA contributor: fullname: Hosoda – volume: 75 start-page: 616 year: 2007 end-page: 626 ident: bib0055 article-title: Differentiation of cardiomyocytes requires functional serine residues within the amino-terminal domain of desmin publication-title: Differentiation contributor: fullname: Weitzer – volume: 377 start-page: 46 year: 2008 end-page: 51 ident: bib0050 article-title: Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells publication-title: Biochem Biophys Res Commun contributor: fullname: Chan – volume: 459 start-page: 654 year: 2009 end-page: 655 ident: bib0090 article-title: Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors publication-title: Nature contributor: fullname: Bruneau – volume: 231 start-page: 39 year: 2006 end-page: 49 ident: bib0075 article-title: Mesenchymal stem cells and the treatment of cardiac disease publication-title: Exp Biol Med contributor: fullname: Erices – volume: 23 start-page: 845 year: 2005 end-page: 856 ident: bib0005 article-title: Regenerating the heart publication-title: Nat Biotechnol contributor: fullname: Murry – volume: 314 start-page: 420 year: 2004 end-page: 427 ident: bib0035 article-title: Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes publication-title: Biochem Biophys Res Commun contributor: fullname: Collas – volume: 103 start-page: 697 year: 1999 end-page: 705 ident: bib0045 article-title: Cardiomyocytes can be generated from marrow stromal cells in vitro publication-title: J Clin Invest contributor: fullname: Pan – volume: 79 start-page: 97 year: 2008 end-page: 108 ident: bib0065 article-title: Depletion of zebrafish essential and regulatory myosin light chains reduces cardiac function through distinct mechanisms publication-title: Cardiovasc Res contributor: fullname: Xu – volume: 126 start-page: 124 year: 2003 end-page: 132 ident: bib0070 article-title: Cardiomyocyte-mediated contact programs human mesenchymal stem cells to express cardiogenic phenotype publication-title: J Thorac Cardiovasc Surg contributor: fullname: Kresh – volume: 68 start-page: 464 year: 2008 end-page: 472 ident: bib0080 article-title: Liposome-mediated transfection with extract from neonatal rat cardiomyocytes induces transdifferentiation of human adipose-derived stem cells into cardiomyocytes publication-title: Scand J Clin Lab Invest contributor: fullname: Krohn – volume: 43 start-page: 377 year: 2007 end-page: 387 ident: bib0015 article-title: Do stem cells in the heart truly differentiate into cardiomyocytes? publication-title: J Mol Cell Cardiol contributor: fullname: Hasenfuss – volume: 20 start-page: 460 year: 2002 end-page: 466 ident: bib0025 article-title: Reprogramming fibroblasts to express T-cell functions using cell extracts publication-title: Nat. Biotechnol contributor: fullname: Collas – volume: 28 start-page: 663 year: 2007 end-page: 671 ident: bib0030 article-title: Neocartilage formation from predifferentiated human adipose derived stem cells in vivo publication-title: Acta Pharmacol Sin contributor: fullname: Lou – volume: 11 start-page: 778 year: 2009 end-page: 792 ident: bib0085 article-title: Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity publication-title: Cytotherapy contributor: fullname: Jongen – volume: 103 start-page: 9226 year: 2006 ident: 10.3109/14653240903548202_bib0010 article-title: Stem cell niches in the adult mouse heart publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.0600635103 contributor: fullname: Urbanek – volume: 20 start-page: 460 year: 2002 ident: 10.3109/14653240903548202_bib0025 article-title: Reprogramming fibroblasts to express T-cell functions using cell extracts publication-title: Nat. Biotechnol doi: 10.1038/nbt0502-460 contributor: fullname: Håkelien – volume: 28 start-page: 663 year: 2007 ident: 10.3109/14653240903548202_bib0030 article-title: Neocartilage formation from predifferentiated human adipose derived stem cells in vivo publication-title: Acta Pharmacol Sin doi: 10.1111/j.1745-7254.2007.00553.x contributor: fullname: Jin – volume: 100 start-page: 1249 year: 2007 ident: 10.3109/14653240903548202_bib0040 article-title: Adipose-derived stem cells for regenerative medicine publication-title: Circ Res doi: 10.1161/01.RES.0000265074.83288.09 contributor: fullname: Gimble – volume: 140 start-page: 138 year: 2005 ident: 10.3109/14653240903548202_bib0020 article-title: Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation potentialities publication-title: Bull Exp Biol Med doi: 10.1007/s10517-005-0430-z contributor: fullname: Romanov – volume: 79 start-page: 97 year: 2008 ident: 10.3109/14653240903548202_bib0065 article-title: Depletion of zebrafish essential and regulatory myosin light chains reduces cardiac function through distinct mechanisms publication-title: Cardiovasc Res doi: 10.1093/cvr/cvn073 contributor: fullname: Chen – volume: 459 start-page: 654 year: 2009 ident: 10.3109/14653240903548202_bib0090 article-title: Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors publication-title: Nature doi: 10.1038/nature08039 contributor: fullname: Takeuchi – volume: 314 start-page: 420 year: 2004 ident: 10.3109/14653240903548202_bib0035 article-title: Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2003.12.109 contributor: fullname: Gaustad – volume: 68 start-page: 464 year: 2008 ident: 10.3109/14653240903548202_bib0080 article-title: Liposome-mediated transfection with extract from neonatal rat cardiomyocytes induces transdifferentiation of human adipose-derived stem cells into cardiomyocytes publication-title: Scand J Clin Lab Invest doi: 10.1080/00365510701836907 contributor: fullname: Schimrosczyk – volume: 43 start-page: 377 year: 2007 ident: 10.3109/14653240903548202_bib0015 article-title: Do stem cells in the heart truly differentiate into cardiomyocytes? publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2007.07.056 contributor: fullname: Guan – volume: 11 start-page: 778 year: 2009 ident: 10.3109/14653240903548202_bib0085 article-title: Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity publication-title: Cytotherapy doi: 10.3109/14653240902988818 contributor: fullname: Koninckx – volume: 23 start-page: 845 year: 2005 ident: 10.3109/14653240903548202_bib0005 article-title: Regenerating the heart publication-title: Nat Biotechnol doi: 10.1038/nbt1117 contributor: fullname: Laflamme – volume: 377 start-page: 46 year: 2008 ident: 10.3109/14653240903548202_bib0050 article-title: Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2008.09.076 contributor: fullname: Moore – volume: 75 start-page: 616 year: 2007 ident: 10.3109/14653240903548202_bib0055 article-title: Differentiation of cardiomyocytes requires functional serine residues within the amino-terminal domain of desmin publication-title: Differentiation doi: 10.1111/j.1432-0436.2007.00163.x contributor: fullname: Höllrigl – volume: 231 start-page: 39 year: 2006 ident: 10.3109/14653240903548202_bib0075 article-title: Mesenchymal stem cells and the treatment of cardiac disease publication-title: Exp Biol Med doi: 10.1177/153537020623100105 contributor: fullname: Miguel – volume: 126 start-page: 124 year: 2003 ident: 10.3109/14653240903548202_bib0070 article-title: Cardiomyocyte-mediated contact programs human mesenchymal stem cells to express cardiogenic phenotype publication-title: J Thorac Cardiovasc Surg doi: 10.1016/S0022-5223(03)00074-6 contributor: fullname: Rangappa – volume: 353 start-page: 665 year: 2007 ident: 10.3109/14653240903548202_bib0060 article-title: Genetically selected stem cells from human adipose tissue express cardiac markers publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2006.12.103 contributor: fullname: Bai – volume: 103 start-page: 697 year: 1999 ident: 10.3109/14653240903548202_bib0045 article-title: Cardiomyocytes can be generated from marrow stromal cells in vitro publication-title: J Clin Invest doi: 10.1172/JCI5298 contributor: fullname: Makino |
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| SubjectTerms | adipose stem cells Adipose Tissue - cytology Adult Adult Stem Cells - cytology Adult Stem Cells - physiology Advanced Basic Science Biomarkers - metabolism cardiomyogenic differentiation Cell Differentiation - physiology cell extract Cell Lineage Cell Transdifferentiation Cells, Cultured Heart Atria - cytology Humans Myocardium - cytology Myocardium - metabolism Myocytes, Cardiac - cytology Myocytes, Cardiac - physiology Other transdifferentiation |
| Title | Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes |
| URI | https://www.clinicalkey.es/playcontent/1-s2.0-S1465324910703959 https://dx.doi.org/10.3109/14653240903548202 https://www.ncbi.nlm.nih.gov/pubmed/20230311 https://search.proquest.com/docview/733858973 |
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