Formation of covalently closed circular DNA in Hep38.7-Tet cells, a tetracycline inducible hepatitis B virus expression cell line

• Published in: Biochemical and biophysical research communications Vol. 452; no. 3; pp. 315 - 321
• Main Authors: Ogura, Naoki, Watashi, Koichi, Noguchi, Toru, Wakita, Takaji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.09.2014
• Subjects: Antiviral; Antiviral Agents - pharmacology; cccDNA; Cell Line, Transformed - drug effects; Cell Line, Transformed - metabolism; Cell Line, Transformed - virology; circular DNA; cultured cells; DNA, Circular - genetics; DNA, Circular - metabolism; DNA, Viral - genetics; DNA, Viral - metabolism; Founder Effect; Gene Expression; HBV; hepatitis B antigens; Hepatitis B e Antigens - biosynthesis; Hepatitis B e Antigens - genetics; Hepatitis B virus; Hepatitis B virus - drug effects; Hepatitis B virus - genetics; Hepatitis B virus - metabolism; Hepatocytes - drug effects; Hepatocytes - metabolism; Hepatocytes - virology; High-Throughput Screening Assays; Humans; Protein Synthesis Inhibitors - pharmacology; secretion; Small Molecule Libraries - pharmacology; tetracycline; Tetracycline - pharmacology; Virus Replication - drug effects; cccDNA; Antiviral; HBV

•Hep38.7-Tet cells were established by subcloning of HepAD38 cells and selected.•Hep38.7-Tet cells were higher than HepAD38 cells in cccDNA accumulation and HBeAg secretion.•Hep38.7-Tet cells will be powerful tools for analysis of the molecular mechanism of cccDNA formation. Hepatitis B virus (HBV)...