Temporal control over the initiation of cell motility by a regulator of G-protein signaling

The control over the acquisition of cell motility is central for a variety of biological processes in development, homeostasis, and disease. An attractive in vivo model for investigating the regulation of migration initiation is that of primordial germ cells (PGCs) in zebrafish embryos. In this stud...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 111; no. 31; pp. 11389 - 11394
Main Authors Hartwig, Johannes, Tarbashevich, Katsiaryna, Seggewiß, Jochen, Stehling, Martin, Bandemer, Jan, Grimaldi, Cecilia, Paksa, Azadeh, Groß-Thebing, Theresa, Meyen, Dana, Raz, Erez
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 05.08.2014
National Acad Sciences
Subjects
Animals
B lymphocytes
Biological Sciences
Cadherins
Cadherins - metabolism
Cell motility
Cell Movement - genetics
Cell Polarity - genetics
Cells
Danio rerio
Embryos
Gene expression regulation
Gene Expression Regulation, Developmental
Germ cells
Germ Cells - cytology
Germ Cells - metabolism
Glycoproteins
Homeostasis
Messenger RNA
Molecules
RGS proteins
RGS Proteins - genetics
RGS Proteins - metabolism
Ribonucleic acid
RNA
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal transduction
Signal Transduction - genetics
Somatic cells
Time Factors
Zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
rgs protein
cell adhesion
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Summary:The control over the acquisition of cell motility is central for a variety of biological processes in development, homeostasis, and disease. An attractive in vivo model for investigating the regulation of migration initiation is that of primordial germ cells (PGCs) in zebrafish embryos. In this study, we show that, following PGC specification, the cells can polarize but do not migrate before the time chemokine-encoded directional cues are established. We found that the regulator of G-protein signaling 14a protein, whose RNA is a newly identified germ plasm component, regulates the temporal relations between the appearance of the guidance molecules and the acquisition of cellular motility by regulating E-cadherin levels.
Bibliography:http://dx.doi.org/10.1073/pnas.1400043111
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Author contributions: M.S. performed FACS experiments; J.H., K.T., and J.S. performed bioinformatics experiments; K.T. performed rgs14a qPCR and determination of zygotic expression; A.P. and T.G.-T. performed RNAscope labeling; J.B. and C.G. analyzed protrusions during e-cadherin overexpression and cloning of E-Cadherin constructs; D.M. generated lifeact-GFP fish; J.H. performed all other experiments; J.H., K.T., and E.R. designed the research; and J.H., K.T., and E.R. wrote the paper.
Edited by Igor B. Dawid, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, and approved July 2, 2014 (received for review January 3, 2014)
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1400043111