Heterogeneous activation of a slow myosin gene in proliferating myoblasts and differentiated single myofibers

• Published in: Developmental biology Vol. 402; no. 1; pp. 72 - 80
• Main Authors: Wang, Jing-hua, Wang, Qiao-jing, Wang, Chao, Reinholt, Brad, Grant, Alan L., Gerrard, David E., Kuang, Shihuan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2015
• Subjects: Animals; Cardiotoxins - chemistry; Cell Differentiation; Cell Nucleus - metabolism; Cell Separation; Cells, Cultured; Cre/LoxP; Flow Cytometry; Gene Expression Regulation, Developmental; Genes, Reporter; Green Fluorescent Proteins - metabolism; Mice; Mice, Transgenic; Muscle fiber type; Muscle Fibers, Slow-Twitch - metabolism; Muscle, Skeletal - metabolism; Muscles - cytology; Muscles - metabolism; Muscles - pathology; Myoblasts - cytology; Myogenesis; Myosin Heavy Chains - metabolism; Myosins - chemistry; PAX7 Transcription Factor - metabolism; Regeneration; Satellite cells; Satellite Cells, Skeletal Muscle - cytology; MYL; TdTomato; Myod; CTX; Satellite cells; FACS; EDL; TA; Myogenesis; BSA; DMEM; SOL; Regeneration; Pax7; FBS; Muscle fiber type; MYH; RFP; Cre/LoxP; EYFP

Each skeletal muscle contains a fixed ratio of fast and slow myofibers that are distributed in a stereotyped pattern to achieve a specific motor function. How myofibers are specified during development and regeneration is poorly understood. Here we address this question using transgenic reporter mic...