A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells

• Published in: Lab on a chip Vol. 13; no. 23; pp. 4663 - 4673
• Main Authors: Ges, Igor A, Brindley, Rebecca L, Currie, Kevin P. M, Baudenbacher, Franz J
• Format: Journal Article
• Language: English
• Published: England 07.12.2013
• Subjects: Animals; Arrays; Calcium - metabolism; Carbachol - pharmacology; Catecholamines - analysis; Catecholamines - secretion; Cattle; Cells, Cultured; Chromaffin Cells - cytology; Chromaffin Cells - drug effects; Chromaffin Cells - secretion; Chromatography, High Pressure Liquid; Dimethylpolysiloxanes; Electrochemical Techniques; Electrodes; Kinetics; Microfluidic Analytical Techniques - instrumentation; Pituitary Adenylate Cyclase-Activating Polypeptide - pharmacology; Platinum - chemistry; Potassium Chloride - pharmacology; Silicon - chemistry; Stimulation, Chemical

Release of neurotransmitters and hormones by calcium-regulated exocytosis is a fundamental cellular process that is disrupted in a variety of psychiatric, neurological, and endocrine disorders. As such, there is significant interest in targeting neurosecretion for drug and therapeutic development, e...