On-demand biomanufacturing through synthetic biology approach

• Published in: Materials today bio Vol. 18; p. 100518
• Main Authors: Tang, Chenwang, Wang, Lin, Zang, Lei, Wang, Qing, Qi, Dianpeng, Dai, Zhuojun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2023; Elsevier
• Subjects: Biomanufacturing; Cell-free system; Engineered microorganisms; Living Materials edited by Chao Zhong; On-demand; Portability; Synthetic biology; Portability; Synthetic biology; Engineered microorganisms; Biomanufacturing; Cell-free system; On-demand
• ISSN: 2590-0064; 2590-0064
• DOI: 10.1016/j.mtbio.2022.100518

Biopharmaceuticals including protein therapeutics, engineered protein-based vaccines and monoclonal antibodies, are currently the mainstay products of the biotechnology industry. However, the need for specialized equipment and refrigeration during production and distribution poses challenges for the delivery of these technologies to the field and low-resource area. With the development of synthetic biology, multiple studies rewire the cell-free system or living cells to impact the portable, on-site and on-demand manufacturing of biomolecules. Here, we review these efforts and suggest future directions. On demand biomanufacturing. In this review, we summarize approaches that consisting of cell-free protein synthesis (CFPS) and living cellular hosts to achieve the on demand biomanufacturing of vaccines, antibodies, enzymes, proteins or small molecules drugs. [Display omitted]