Optic Nerve Crush in Mice to Study Retinal Ganglion Cell Survival and Regeneration

• Published in: Bio-protocol Vol. 10; no. 6
• Main Authors: Cameron, Evan, Xia, Xin, Galvao, Joana, Ashouri, Masoumeh, Kapiloff, Michael, Goldberg, Jeffrey
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 20.03.2020; Bio-protocol LLC
• Subjects: Biology; Methods; Glaucoma; Neuroprotection; Regeneration; AAV2; Degeneration; Retinal ganglion cells (RGCs)
• ISSN: 2331-8325; 2331-8325
• DOI: 10.21769/BioProtoc.3559

In diseases such as glaucoma, the failure of retinal ganglion cell (RGC) neurons to survive or regenerate their optic nerve axons underlies partial and, in some cases, complete vision loss. Optic nerve crush (ONC) serves as a useful model not only of traumatic optic neuropathy but also of glaucomato...