Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles

• Published in: Materials today bio Vol. 9; p. 100091
• Main Authors: Lee, Mei-Hwa, Lin, Cheng-Chih, Thomas, James L., Li, Jin-An, Lin, Hung-Yin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2021; Elsevier
• Subjects: dCas9-VPR:gRNA ribonucleoproteins; Full Length; Induced pluripotent stem cell; Magnetic peptide-imprinted nanoparticles; Induced pluripotent stem cell; dCas9-VPR:gRNA ribonucleoproteins; Magnetic peptide-imprinted nanoparticles
• ISSN: 2590-0064; 2590-0064
• DOI: 10.1016/j.mtbio.2020.100091

Induced pluripotent stem cells are usually derived by reprogramming transcription factors (OSKM), such as octamer-binding transcription factor 4 (OCT4), (sex determining region Y)-box 2 (SOX2), Krüppel-like factor 4 (KLF4), and cellular proto-oncogene (c-Myc). However, the genomic integration of tra...