DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks

• Published in: Molecular cell Vol. 63; no. 4; pp. 633 - 646
• Main Authors: van Overbeek, Megan, Capurso, Daniel, Carter, Matthew M., Thompson, Matthew S., Frias, Elizabeth, Russ, Carsten, Reece-Hoyes, John S., Nye, Christopher, Gradia, Scott, Vidal, Bastien, Zheng, Jiashun, Hoffman, Gregory R., Fuller, Christopher K., May, Andrew P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.08.2016
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; CRISPR-Associated Protein 9; CRISPR-Cas Systems; DNA Breaks, Double-Stranded; DNA End-Joining Repair; Endonucleases - genetics; Endonucleases - metabolism; Gene Editing; Gene Expression Profiling - methods; HCT116 Cells; HEK293 Cells; Humans; K562 Cells; RNA Interference; RNA, Guide, CRISPR-Cas Systems - genetics; RNA, Guide, CRISPR-Cas Systems - metabolism; Time Factors; Transfection

The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the widespread adoption of CRISPR-Cas9 technology to induce DSBs for genome engineering, the resulting repair products have not been...