Identification and quantification of glutathione and phytochelatins from Chlorella vulgaris by RP-HPLC ESI-MS/MS and oxygen-free extraction

• Published in: Analytical and bioanalytical chemistry Vol. 395; no. 3; pp. 809 - 817
• Main Authors: Simmons, Denina B. D, Hayward, Allison R, Hutchinson, Thomas C, Emery, R. J. Neil
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.10.2009; Springer-Verlag; Springer
• Subjects: Adjustment; air; analysis; Analytical Chemistry; Applied sciences; argon (noble gases); Auto-oxidation; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chlorella vulgaris; Chlorella vulgaris - chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Chromatography, High Pressure Liquid - methods; Cost analysis; cost effectiveness; Degradation; detection limit; ESI-MS/MS; Exact sciences and technology; Extraction; Food Science; fungi; Global environmental pollution; Glutathione; Glutathione - analysis; Glutathione - isolation & purification; heavy metals; HPLC-MS; Internal standard; ionization; isolation & purification; Laboratory Medicine; Linearity; methods; Monitoring/Environmental Analysis; Original Paper; Other chromatographic methods; oxidation; Peptides; phytochelatins; Phytochelatins - chemistry; Phytochelatins - isolation & purification; Pollution; reversed-phase high performance liquid chromatography; Sensitivity and Specificity; spectrometers; Spectrometric and optical methods; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry; Tandem Mass Spectrometry - methods; Phytochelatins; ESI-MS/MS; HPLC-MS; Internal standard; Auto-oxidation; Cysteine; Peptides; HPLC chromatography; Glycine; Electrospray; Quadrupole spectrometer; Sample preparation; Indirect method; Detection limit; Oxidation; Argon; Quantitative analysis; Glutathione; Oxygen; Algae; Chlorella vulgaris; Air; Calibration; Chemical ionization; Heavy metal; Reversed phase chromatography; Sensitivity; Linearity; Mass spectrometry MS/MS; Environment; Mass spectrometry

Phytochelatins are short, cysteine-containing, detoxification peptides produced by plants, algae, and fungi in response to heavy metal exposure. These peptides auto-oxidize easily. Current extraction protocols do not adequately address losses of phytochelatins because of their oxidation and the use...