First description of the endogenous life cycle of Hypoderma sinense affecting yaks and cattle in China

• Published in: Medical and veterinary entomology Vol. 20; no. 3; pp. 325 - 328
• Main Authors: Otranto, D, Paradies, P, Testini, G, Lia, R.P, Giangaspero, A, Traversa, D, Colwell, D.D
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2006
• Subjects: Animals; biology; body size; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - parasitology; China; China - epidemiology; cox1; cox1 gene; Diptera; Diptera - anatomy & histology; Diptera - classification; Diptera - physiology; endogenous life cycle; Female; Hypoderma; Hypoderma lineatum; Hypoderma sinense; hypodermosis; Hypodermyiasis - epidemiology; Hypodermyiasis - parasitology; Hypodermyiasis - veterinary; insect morphology; insect taxonomy; Larva - anatomy & histology; Larva - classification; larvae; larval migration; life cycle (organisms); Male; mitochondrial DNA; molecular identification; molecular systematics; myiasis; Oestridae; parasite identification; restriction fragment length polymorphism; Ruminantia; tissue distribution; warble flies; yaks; China; China, People's Rep
• ISSN: 0269-283X; 1365-2915
• DOI: 10.1111/j.1365-2915.2006.00634.x

Larvae belonging to five species of Hypoderma spp. (Diptera, Oestridae) cause myiasis in wild and domestic ruminants that is characterized by migrations within deep tissues. In China hypodermosis is one of the most important arthropod diseases affecting ruminants and, moreover, represents a significant zoonosis, with numerous reports of Hypoderma spp. affecting farmers. Recently, a sixth species, Hypoderma sinense Pleske, has been rediscovered but the endogenous migration pathway within the host body is completely unknown and it represents a major constraint for the control of larval infection. In December 2003 a total of 165 larval stages of Hypoderma spp. were collected from different anatomical sites of 40 yaks slaughtered at an abattoir in the province of Gansu, China. The morphological characters and size of the recovered larvae were used to infer migratory routes and 45 specimens were also subjected to a polymerase chain reaction (PCR) assay of cox1 mtDNA and amplicons sequenced. All the larvae molecularly processed were identified as H. sinense and sequence identity was confirmed by a PCR-restriction fragment length polymorphism (PCR-RFLP) tool carried out using BfaI and HinfI endonucleases. The finding of H. sinense larvae only in the oesophagus or both in oesophagus and subcutaneous tissue of 12 and 15 animals, respectively, indicates that H. sinense larvae migrate through the oesophagus similarly to Hypoderma lineatum (De Villers). The description of the endogenous life cycle of H. sinense will help to determine the timing of specific treatment programmes to guarantee the improvement of animal welfare and health, thus resulting in an increase in livestock production in China.