Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan

During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic sy...

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Published in	Japanese Journal of Infectious Diseases Vol. 73; no. 4; pp. 304 - 307
Main Authors	Nakatsu, Yuichiro, Katoh, Hiroshi, Matsuyama, Shutoku, Shirato, Kazuya, Kato, Fumihiro, Kageyama, Tsutomu, Nao, Naganori, Saito, Shinji, Sakata, Masafumi, Takeda, Makoto, Katano, Harutaka, Mori, Yoshio, Takayama, Ikuyo
Format	Journal Article
Language	English
Published	Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 31.07.2020
Subjects	Betacoronavirus - genetics Clinical Laboratory Techniques - methods Coronavirus Infections - diagnosis COVID-19 COVID-19 Testing COVID-19 Vaccines Humans Japan nCoV-2019 nested RT-PCR novel coronavirus 2019 Pandemics Pneumonia, Viral - diagnosis Polyproteins real-time RT-PCR Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis SARS-CoV-2 Spike Glycoprotein, Coronavirus - genetics Viral Proteins - genetics Japan nCoV-2019 nested RT-PCR real-time RT-PCR novel coronavirus 2019
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Abstract	During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan.
AbstractList	During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan. During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan.During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan.
Author	Nakatsu, Yuichiro Sakata, Masafumi Katoh, Hiroshi Takeda, Makoto Katano, Harutaka Nao, Naganori Kageyama, Tsutomu Saito, Shinji Matsuyama, Shutoku Shirato, Kazuya Mori, Yoshio Takayama, Ikuyo Kato, Fumihiro
Author_xml	– sequence: 1 fullname: Nakatsu, Yuichiro organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Katoh, Hiroshi organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Matsuyama, Shutoku organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Shirato, Kazuya organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Kato, Fumihiro organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Kageyama, Tsutomu organization: Influenza Virus Research Center, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Nao, Naganori organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Saito, Shinji organization: Influenza Virus Research Center, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Sakata, Masafumi organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Takeda, Makoto organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Katano, Harutaka organization: Department of Pathology National Institute of Infectious Disease, Japan – sequence: 1 fullname: Mori, Yoshio organization: Department of Virology III, National Institute of Infectious Disease, Japan – sequence: 1 fullname: Takayama, Ikuyo organization: Influenza Virus Research Center, National Institute of Infectious Disease, Japan
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/32074516$$D View this record in MEDLINE/PubMed
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Publisher	National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
Publisher_xml	– name: National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
References	6. Shirato K, Nao N, Matsuyama S, et al. An ultra-rapid real-time RT-PCR method for detecting Middle East respiratory syndrome coronavirus using a mobile PCR device, PCR1100. Jpn J Infect Dis. 2020;73:181-6. 1. Lai S, Bogoch I, Watts A, et al. Preliminary risk analysis of 2019 novel coronavirus spread within and beyond China. Available at <https://www.worldpop.org/resources/docs/china/WorldPopcoronavirus-spread-risk-analysis-v1-25Jan.pdf> Accessed February 12, 2020. 2. Nao N, Shirato K, Matsuyama S, et al. Detection of WN-Human1 sequence from clinical specimen. Available at <https://www.niid.go.jp/niid/images/vir3/nCoV/Method_NIID_20200114_Na.pdf> Accessed February 12, 2020. 3. Katano H, Suzuki T. Detection of 2019-novel coronavirus sequence from clinical specimen. Available at <https://www.niid.go.jp/niid/images/pathol/pdf/Detection_of_nCoV_report200121.pdf> Accessed February 12, 2020. 7. World Health Organization(WHO). Novel Coronavirus (2019- nCoV) situation reports. Available at <https://www.who.int/emergencies/diseases/novel-coronavirus-2019/situationreports/>Accessed February 12, 2020. 5. Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045. 4. Nao N, Shirato K, Katano H, et al. Detection of second case of 2019-nCoV infection in Japan(corrected version). Available at <https://www.niid.go.jp/niid/images/vir3/nCoV/method-niid-20200123-2_erratum.pdf> Accessed February 12, 2020. 1 2 3 4 5 6 7
References_xml	– reference: 3. Katano H, Suzuki T. Detection of 2019-novel coronavirus sequence from clinical specimen. Available at <https://www.niid.go.jp/niid/images/pathol/pdf/Detection_of_nCoV_report200121.pdf> Accessed February 12, 2020. – reference: 7. World Health Organization(WHO). Novel Coronavirus (2019- nCoV) situation reports. Available at <https://www.who.int/emergencies/diseases/novel-coronavirus-2019/situationreports/>Accessed February 12, 2020. – reference: 2. Nao N, Shirato K, Matsuyama S, et al. Detection of WN-Human1 sequence from clinical specimen. Available at <https://www.niid.go.jp/niid/images/vir3/nCoV/Method_NIID_20200114_Na.pdf> Accessed February 12, 2020. – reference: 4. Nao N, Shirato K, Katano H, et al. Detection of second case of 2019-nCoV infection in Japan(corrected version). Available at <https://www.niid.go.jp/niid/images/vir3/nCoV/method-niid-20200123-2_erratum.pdf> Accessed February 12, 2020. – reference: 5. Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045. – reference: 6. Shirato K, Nao N, Matsuyama S, et al. An ultra-rapid real-time RT-PCR method for detecting Middle East respiratory syndrome coronavirus using a mobile PCR device, PCR1100. Jpn J Infect Dis. 2020;73:181-6. – reference: 1. Lai S, Bogoch I, Watts A, et al. Preliminary risk analysis of 2019 novel coronavirus spread within and beyond China. Available at <https://www.worldpop.org/resources/docs/china/WorldPopcoronavirus-spread-risk-analysis-v1-25Jan.pdf> Accessed February 12, 2020. – ident: 2 – ident: 3 – ident: 4 – ident: 6 doi: 10.7883/yoken.JJID.2019.400 – ident: 1 – ident: 5 doi: 10.2807/1560-7917.ES.2020.25.3.2000045 – ident: 7
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Snippet	During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between...
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SubjectTerms	Betacoronavirus - genetics Clinical Laboratory Techniques - methods Coronavirus Infections - diagnosis COVID-19 COVID-19 Testing COVID-19 Vaccines Humans Japan nCoV-2019 nested RT-PCR novel coronavirus 2019 Pandemics Pneumonia, Viral - diagnosis Polyproteins real-time RT-PCR Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis SARS-CoV-2 Spike Glycoprotein, Coronavirus - genetics Viral Proteins - genetics
Title	Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan
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