Development and application of an indirect enzyme-linked immunosorbent assay for serological survey of Japanese encephalitis virus infection in dogs

• Published in: Journal of virological methods Vol. 187; no. 1; pp. 85 - 89
• Main Authors: Shimoda, Hiroshi, Inthong, Natnaree, Noguchi, Keita, Terada, Yutaka, Nagao, Yumiko, Shimojima, Masayuki, Takasaki, Tomohiko, Rerkamnuaychoke, Worawut, Maeda, Ken
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2013
• Subjects: Animals; antibodies; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; antigens; Cell Line; Cercopithecus aethiops; correlation; Data processing; Dog; dog diseases; Dog Diseases - diagnosis; Dog Diseases - immunology; Dogs; Encephalitis; Encephalitis Virus, Japanese - immunology; Encephalitis, Japanese - diagnosis; Encephalitis, Japanese - immunology; Encephalitis, Japanese - veterinary; Enzyme-linked immunosorbent assay; Enzyme-linked immunosorbent assay (ELISA); Enzyme-Linked Immunosorbent Assay - veterinary; Female; horses; human diseases; humans; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin M; Immunoglobulin M - blood; Infection; Japan; Japanese encephalitis virus; Japanese encephalitis virus (JEV); Male; neutralization tests; risk; risk assessment; Sensitivity and Specificity; Seroepidemiologic Studies; Serological survey; Serological surveys; seroprevalence; Thailand; Vero Cells; virion; Thailand; Japan; Japanese encephalitis virus (JEV); Enzyme-linked immunosorbent assay (ELISA); Dog; Serological survey

► A simple method for detecting JEV antibodies in sera from dogs was developed. ► This ELISA is useful for assessment of the human risk of JEV infection. ► Both ELISA and virus neutralization should be used for serological surveys of JEV. Japanese encephalitis virus (JEV) causes serious acute enceph...