Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 109; no. 19; pp. 7499 - 7504
• Main Authors: Qi, Yingchuan B, Garren, Emma J, Shu, Xiaokun, Tsien, Roger Y, Jin, Yishi
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 08.05.2012; National Acad Sciences
• Subjects: acetylcholine; Acetylcholine receptors; Amino acids; Animals; Animals, Genetically Modified; Biological Sciences; blue light; Caenorhabditis elegans; Caenorhabditis elegans - cytology; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Caspase; caspases; Caspases - genetics; Caspases - metabolism; Cell Death - radiation effects; Cell Survival - radiation effects; Cells; Convulsions; death; Developmental stages; Dose-Response Relationship, Radiation; Flavoproteins - genetics; Flavoproteins - metabolism; Fluorescence; Genetic code; humans; Illumination; Interneurons; Interneurons - cytology; Interneurons - metabolism; Interneurons - radiation effects; Larvae; Light; lighting; Locomotion; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Medical genetics; Membrane Proteins - genetics; Membrane Proteins - metabolism; Microscopy, Confocal; Microscopy, Fluorescence; Mitochondria; Mitochondria - metabolism; Motor neurons; Motor Neurons - cytology; Motor Neurons - metabolism; Motor Neurons - radiation effects; Mutation; Nematodes; Neurons; Neurons - cytology; Neurons - metabolism; Neurons - radiation effects; Oxygen; Phagocytes; Proteins; Receptors, Nicotinic - genetics; Receptors, Nicotinic - metabolism; singlet oxygen; Singlet Oxygen - metabolism; Time Factors; Transgenic animals

We describe a method for light-inducible and tissue-selective cell ablation using a genetically encoded photosensitizer, miniSOG (mini singlet oxygen generator). miniSOG is a newly engineered fluorescent protein of 106 amino acids that generates singlet oxygen in quantum yield upon blue-light illumi...