Free NADH Concentration Is Kept Constant in Plant Mitochondria under Different Metabolic Conditions

• Published in: The Plant cell Vol. 18; no. 3; pp. 688 - 698
• Main Authors: Kasimova, Marina R, Grigiene, Jurgita, Krab, Klaas, Hagedorn, Peter H, Flyvbjerg, Henrik, Andersen, Peter E, Møller, Ian M
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Biologists 01.03.2006
• Subjects: Adenosine diphosphate; binding capacity; Binding sites; Calorimetry; cell respiration; cytochemistry; cytology; Dehydrogenases; electron transport chain; Emission spectra; Emissions; Enzymes; Fluorescence; Homeostasis; Ligands; Malates; Malates - metabolism; metabolism; Mitochondria; Mitochondria - metabolism; Models, Biological; NAD; NAD (coenzyme); NAD - metabolism; Oxidation; Oxidation-Reduction; Plant cells; potatoes; Solanum tuberosum; Solanum tuberosum - cytology; Solanum tuberosum - metabolism; Spectrometry, Fluorescence; Tubers; Wavelengths

The reduced coenzyme NADH plays a central role in mitochondrial respiratory metabolism. However, reports on the amount of free NADH in mitochondria are sparse and contradictory. We first determined the emission spectrum of NADH bound to proteins using isothermal titration calorimetry combined with f...