Occurrence of acetylcholine-hydrolyzing activity at the stele-cortex interface
Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and AChE have previously been demonstrated to occur in plants. In this work, we detected AChE at the interface between stele and cortex of the mesocotyl of Zea...
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Published in | Plant physiology (Bethesda) Vol. 99; no. 1; pp. 130 - 133 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
Rockville, MD
American Society of Plant Physiologists
01.05.1992
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Subjects | |
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Abstract | Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and AChE have previously been demonstrated to occur in plants. In this work, we detected AChE at the interface between stele and cortex of the mesocotyl of Zea mays by measuring hydrolysis of acetylthiocholine and by liberation of labeled acetate from [1-14C]ACh. AChE activity was also detected in a crude membrane fraction. The hydrolytic activity is inhibited by neostigmine. Hydrolysis of ACh was also measured after injection of [1-14C]ACh into kernels of Zea mays and the radioactivity transported into the mesocotyl cortex. A gravity stimulus was then given by placing the plants in a horizontal position. Significantly more radioactivity was found in the lower cortex of horizontally placed seedlings. A working hypothesis is presented for the involvement of ACh and AChE in the tropic response of Z. mays seedlings |
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AbstractList | Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and AChE have previously been demonstrated to occur in plants. In this work, we detected AChE at the interface between stele and cortex of the mesocotyl of Zea mays by measuring hydrolysis of acetylthiocholine and by liberation of labeled acetate from [1-(14)C]ACh. AChE activity was also detected in a crude membrane fraction. The hydrolytic activity is inhibited by neostigmine. Hydrolysis of ACh was also measured after injection of [1-(14)C]ACh into kernels of Zea mays and the radioactivity transported into the mesocotyl cortex. A gravity stimulus was then given by placing the plants in a horizontal position. Significantly more radioactivity was found in the lower cortex of horizontally placed seedlings. A working hypothesis is presented for the involvement of ACh and AChE in the tropic response of Z. mays seedlings. Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and AChE have previously been demonstrated to occur in plants. In this work, we detected AChE at the interface between stele and cortex of the mesocotyl of Zea mays by measuring hydrolysis of acetylthiocholine and by liberation of labeled acetate from [1-14C]ACh. AChE activity was also detected in a crude membrane fraction. The hydrolytic activity is inhibited by neostigmine. Hydrolysis of ACh was also measured after injection of [1-14C]ACh into kernels of Zea mays and the radioactivity transported into the mesocotyl cortex. A gravity stimulus was then given by placing the plants in a horizontal position. Significantly more radioactivity was found in the lower cortex of horizontally placed seedlings. A working hypothesis is presented for the involvement of ACh and AChE in the tropic response of Z. mays seedlings Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and AChE have previously been demonstrated to occur in plants. In this work, we detected AChE at the interface between stele and cortex of the mesocotyl of Zea mays by measuring hydrolysis of acetylthiocholine and by liberation of labeled acetate from [1-14C]ACh. AChE activity was also detected in a crude membrane fraction. The hydrolytic activity is inhibited by neostigmine. Hydrolysis of ACh was also measured after injection of [1-14C]ACh into kernels of Zea mays and the radioactivity transported into the mesocotyl cortex. A gravity stimulus was then given by placing the plants in a horizontal position. Significantly more radioactivity was found in the lower cortex of horizontally placed seedlings. A working hypothesis is presented for the involvement of ACh and AChE in the tropic response of Z. mays seedlings. Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and acetylcholinesterase have previously been demonstrated to occur in plants. In this work, we detected acetylcholinesterase at the interface between stele and cortex of the mesocotyl of Zea mays by measuring hydrolysis of acetylthiocholine and by liberation of labeled acetate from (1- super(14)C)ACh. |
Author | Momonoki, Y.S. (Tokyo University of Agriculture, Hokkaido, Japan) |
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Keywords | Plant tissue Translocation Monocotyledones Zea mays Enzyme Acetylcholinesterase Cereal crop Hydrolysis Enzymatic activity Gramineae Angiospermae Plasmodesm Spermatophyta Acetylcholine Localization |
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Snippet | Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and AChE have... Acetylcholine (ACh) is a chemical transmitter serving to propagate an electrical perturbation across the synaptic junctions of animals. ACh and... |
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SubjectTerms | Acetates ACETILCOLINA ACETYLCHOLINE acetylcholinesterase ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Biological and medical sciences Cell membranes Cholinergic receptors COMPOSICION QUIMICA COMPOSITION CHIMIQUE CORTEX COTILEDONES COTYLEDON detection Enzymes ESTELA Fundamental and applied biological sciences. Psychology HIDROLISIS HYDROLYSE Hydrolysis INTERFACE Metabolism OXIRREDUCION OXYDOREDUCTION Pain PHENOMENE D'INTERFACE Plant cells Plant physiology and development Plants Radioactive decay Root stele Seedlings STELE ZEA MAYS |
Title | Occurrence of acetylcholine-hydrolyzing activity at the stele-cortex interface |
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