Functional and Comparative Genomic Analyses of an Operon Involved in Fructooligosaccharide Utilization by Lactobacillus acidophilus
Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS util...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 100; no. 15; pp. 8957 - 8962 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
22.07.2003
National Acad Sciences |
Subjects | |
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Abstract | Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS utilization by Lactobacillus species. Analysis of the L. acidophilus NCFM genome revealed an msm locus composed of a transcriptional regulator of the LacI family, a four-component ATP-binding cassette (ABC) transport system, a fructosidase, and a sucrose phosphorylase. Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some specificity for nonreadily fermentable sugars. Additionally, expression was repressed by glucose but not by fructose, suggesting catabolite repression via two cre-like sequences identified in the promoter-operator region. Insertional inactivation of the genes encoding the ABC transporter substrate-binding protein and the fructosidase reduced the ability of the mutants to grow on FOS. Comparative analysis of gene architecture within this cluster revealed a high degree of synteny with operons in Streptococcus mutans and Streptococcus pneumoniae. However, the association between a fructosidase and an ABC transporter is unusual and may be specific to L. acidophilus. This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract. |
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AbstractList | Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS utilization by Lactobacillus species. Analysis of the L. acidophilus NCFM genome revealed an msm locus composed of a transcriptional regulator of the LacI family, a four-component ATP-binding cassette (ABC) transport system, a fructosidase, and a sucrose phosphorylase. Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some specificity for nonreadily fermentable sugars. Additionally, expression was repressed by glucose but not by fructose, suggesting catabolite repression via two cre-like sequences identified in the promoter-operator region. Insertional inactivation of the genes encoding the ABC transporter substrate-binding protein and the fructosidase reduced the ability of the mutants to grow on FOS. Comparative analysis of gene architecture within this cluster revealed a high degree of synteny with operons in Streptococcus mutans and Streptococcus pneumoniae. However, the association between a fructosidase and an ABC transporter is unusual and may be specific to L. acidophilus. This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract. Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS utilization by Lactobacillus species. Analysis of the L. acidophilus NCFM genome revealed an msm locus composed of a transcriptional regulator of the Lac I family, a four-component ATP-binding cassette (ABC) transport system, a fructosidase, and a sucrose phosphorylase. Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some specificity for nonreadily fermentable sugars. Additionally, expression was repressed by glucose but not by fructose, suggesting catabolite repression via two cre -like sequences identified in the promoter–operator region. Insertional inactivation of the genes encoding the ABC transporter substrate-binding protein and the fructosidase reduced the ability of the mutants to grow on FOS. Comparative analysis of gene architecture within this cluster revealed a high degree of synteny with operons in Streptococcus mutans and Streptococcus pneumoniae . However, the association between a fructosidase and an ABC transporter is unusual and may be specific to L. acidophilus . This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract. Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS utilization by Lactobacillus species. Analysis of the L. acidophilus NCFM genome revealed an msm locus composed of a transcriptional regulator of the Lac I family, a four-component ATP-binding cassette (ABC) transport system, a fructosidase, and a sucrose phosphorylase. Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some specificity for nonreadily fermentable sugars. Additionally, expression was repressed by glucose but not by fructose, suggesting catabolite repression via two cre -like sequences identified in the promoter–operator region. Insertional inactivation of the genes encoding the ABC transporter substrate-binding protein and the fructosidase reduced the ability of the mutants to grow on FOS. Comparative analysis of gene architecture within this cluster revealed a high degree of synteny with operons in Streptococcus mutans and Streptococcus pneumoniae . However, the association between a fructosidase and an ABC transporter is unusual and may be specific to L. acidophilus . This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract. |
Author | Barrangou, Rodolphe Altermann, Eric Cano, Raul Hutkins, Robert Klaenhammer, Todd R. |
AuthorAffiliation | Genomic Sciences Program and Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC 27695; † Department of Food Science and Technology, University of Nebraska, Lincoln, NE 68583-0919; and ‡ California Polytechnic State University, Environmental Biotechnology Institute, San Luis Obispo, CA 93407 |
AuthorAffiliation_xml | – name: Genomic Sciences Program and Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC 27695; † Department of Food Science and Technology, University of Nebraska, Lincoln, NE 68583-0919; and ‡ California Polytechnic State University, Environmental Biotechnology Institute, San Luis Obispo, CA 93407 |
Author_xml | – sequence: 1 givenname: Rodolphe surname: Barrangou fullname: Barrangou, Rodolphe – sequence: 2 givenname: Eric surname: Altermann fullname: Altermann, Eric – sequence: 3 givenname: Robert surname: Hutkins fullname: Hutkins, Robert – sequence: 4 givenname: Raul surname: Cano fullname: Cano, Raul – sequence: 5 givenname: Todd R. surname: Klaenhammer fullname: Klaenhammer, Todd R. |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/12847288$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1007/s002530051256 10.1128/AEM.66.1.252-256.2000 10.1128/JB.183.16.4709-4717.2001 10.1101/gr.185801 10.1093/nar/26.2.544 10.1016/0014-5793(95)01344-X 10.1093/jac/46.4.603 10.1128/AEM.66.6.2682-2684.2000 10.1046/j.1365-2672.2001.01446.x 10.1128/aem.45.6.1808-1815.1983 10.1093/bioinformatics/17.12.1244 10.1046/j.1365-2672.2000.01070.x 10.1128/IAI.66.8.3736-3743.1998 10.1093/jn/129.7.1407S 10.1128/IAI.69.4.2477-2486.2001 10.1016/S0378-1119(02)00630-3 10.1073/pnas.0337704100 10.1128/AEM.67.9.4361-4364.2001 10.1073/pnas.87.16.6238 10.1016/0923-2508(94)90028-0 10.1046/j.1365-2672.1997.00239.x 10.1093/nar/28.5.1206 10.1128/jb.169.10.4507-4517.1987 10.1271/bbb.56.1451 10.1093/nar/27.23.4636 10.1128/JB.181.6.1924-1926.1999 10.1111/j.1365-2672.1995.tb03176.x 10.1128/AEM.69.4.2217-2222.2003 10.1128/JB.184.16.4475-4488.2002 10.1111/j.1574-6968.1996.tb08346.x 10.1111/j.1574-6976.2000.tb00550.x 10.1002/1097-0134(20010101)42:1<66::AID-PROT70>3.0.CO;2-4 10.1073/pnas.212527599 10.1021/jf990519i 10.1046/j.1365-2958.1998.00764.x 10.1016/S0021-9258(19)38518-7 10.1006/jmbi.2000.4315 10.1007/s00284-001-0044-2 10.1016/0922-338X(89)90250-X 10.1073/pnas.172501299 10.1128/jb.177.24.7011-7018.1995 10.1128/JB.183.17.5110-5121.2001 10.1007/s002840010156 10.1073/pnas.97.26.14668 10.1146/annurev.micro.55.1.709 10.1093/jn/125.6.1401 10.1016/S0022-2836(05)80360-2 10.1016/S0021-9258(18)42880-3 10.1128/iai.60.11.4621-4632.1992 10.1093/nar/22.22.4673 10.1128/JB.184.1.126-133.2002 10.1128/JB.181.9.2863-2871.1999 10.1006/jmbi.1999.2624 |
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Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 Abbreviations: ABC, ATP-binding cassette; cre, catabolite response element; FOS, fructooligosaccharides; MSM, multiple sugar metabolism; PTS, phosphotransferase system; LGT, lateral gene transfer; GI, gastrointestinal; NCFM, North Carolina Food Microbiology. Contributed by Todd R. Klaenhammer, May 8, 2003 To whom correspondence should be addressed. E-mail: klaenhammer@ncsu.edu. Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AY172019, AY172020, and AY177419). |
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References | e_1_3_2_26_2 e_1_3_2_49_2 e_1_3_2_28_2 e_1_3_2_41_2 e_1_3_2_20_2 e_1_3_2_43_2 e_1_3_2_22_2 e_1_3_2_45_2 e_1_3_2_24_2 e_1_3_2_47_2 e_1_3_2_9_2 e_1_3_2_16_2 e_1_3_2_7_2 e_1_3_2_18_2 e_1_3_2_39_2 (e_1_3_2_37_2) 1989; 31 e_1_3_2_1_2 e_1_3_2_54_2 e_1_3_2_10_2 e_1_3_2_31_2 e_1_3_2_52_2 e_1_3_2_5_2 e_1_3_2_12_2 e_1_3_2_33_2 e_1_3_2_3_2 (e_1_3_2_35_2) 1988; 52 e_1_3_2_14_2 e_1_3_2_50_2 e_1_3_2_27_2 e_1_3_2_48_2 e_1_3_2_29_2 e_1_3_2_40_2 e_1_3_2_21_2 e_1_3_2_42_2 e_1_3_2_23_2 e_1_3_2_44_2 e_1_3_2_25_2 e_1_3_2_46_2 e_1_3_2_15_2 e_1_3_2_38_2 e_1_3_2_8_2 e_1_3_2_17_2 e_1_3_2_6_2 e_1_3_2_19_2 e_1_3_2_30_2 e_1_3_2_53_2 e_1_3_2_32_2 e_1_3_2_51_2 e_1_3_2_11_2 e_1_3_2_34_2 e_1_3_2_4_2 e_1_3_2_13_2 e_1_3_2_36_2 e_1_3_2_55_2 e_1_3_2_2_2 |
References_xml | – ident: e_1_3_2_33_2 doi: 10.1007/s002530051256 – ident: e_1_3_2_42_2 doi: 10.1128/AEM.66.1.252-256.2000 – ident: e_1_3_2_47_2 doi: 10.1128/JB.183.16.4709-4717.2001 – ident: e_1_3_2_53_2 doi: 10.1101/gr.185801 – ident: e_1_3_2_13_2 doi: 10.1093/nar/26.2.544 – ident: e_1_3_2_26_2 doi: 10.1016/0014-5793(95)01344-X – ident: e_1_3_2_10_2 doi: 10.1093/jac/46.4.603 – volume: 31 start-page: 537 year: 1989 ident: e_1_3_2_37_2 publication-title: Appl. Microbiol. Biotechnol. – ident: e_1_3_2_6_2 doi: 10.1128/AEM.66.6.2682-2684.2000 – ident: e_1_3_2_11_2 doi: 10.1046/j.1365-2672.2001.01446.x – ident: e_1_3_2_12_2 doi: 10.1128/aem.45.6.1808-1815.1983 – ident: e_1_3_2_17_2 doi: 10.1093/bioinformatics/17.12.1244 – ident: e_1_3_2_40_2 doi: 10.1046/j.1365-2672.2000.01070.x – ident: e_1_3_2_48_2 doi: 10.1128/IAI.66.8.3736-3743.1998 – ident: e_1_3_2_5_2 doi: 10.1093/jn/129.7.1407S – ident: e_1_3_2_54_2 doi: 10.1128/IAI.69.4.2477-2486.2001 – ident: e_1_3_2_43_2 doi: 10.1016/S0378-1119(02)00630-3 – ident: e_1_3_2_2_2 doi: 10.1073/pnas.0337704100 – ident: e_1_3_2_18_2 doi: 10.1128/AEM.67.9.4361-4364.2001 – ident: e_1_3_2_29_2 doi: 10.1073/pnas.87.16.6238 – ident: e_1_3_2_31_2 doi: 10.1016/0923-2508(94)90028-0 – ident: e_1_3_2_8_2 doi: 10.1046/j.1365-2672.1997.00239.x – ident: e_1_3_2_28_2 doi: 10.1093/nar/28.5.1206 – ident: e_1_3_2_34_2 doi: 10.1128/jb.169.10.4507-4517.1987 – ident: e_1_3_2_41_2 doi: 10.1271/bbb.56.1451 – ident: e_1_3_2_14_2 doi: 10.1093/nar/27.23.4636 – ident: e_1_3_2_49_2 doi: 10.1128/JB.181.6.1924-1926.1999 – ident: e_1_3_2_7_2 doi: 10.1111/j.1365-2672.1995.tb03176.x – ident: e_1_3_2_50_2 doi: 10.1128/AEM.69.4.2217-2222.2003 – ident: e_1_3_2_55_2 doi: 10.1128/JB.184.16.4475-4488.2002 – volume: 52 start-page: 2569 year: 1988 ident: e_1_3_2_35_2 publication-title: Agric. Biol. Chem. – ident: e_1_3_2_25_2 doi: 10.1111/j.1574-6968.1996.tb08346.x – ident: e_1_3_2_23_2 doi: 10.1111/j.1574-6976.2000.tb00550.x – ident: e_1_3_2_44_2 doi: 10.1002/1097-0134(20010101)42:1<66::AID-PROT70>3.0.CO;2-4 – ident: e_1_3_2_3_2 doi: 10.1073/pnas.212527599 – ident: e_1_3_2_9_2 doi: 10.1021/jf990519i – ident: e_1_3_2_24_2 doi: 10.1046/j.1365-2958.1998.00764.x – ident: e_1_3_2_45_2 doi: 10.1016/S0021-9258(19)38518-7 – ident: e_1_3_2_22_2 doi: 10.1006/jmbi.2000.4315 – ident: e_1_3_2_38_2 doi: 10.1007/s00284-001-0044-2 – ident: e_1_3_2_36_2 doi: 10.1016/0922-338X(89)90250-X – ident: e_1_3_2_1_2 doi: 10.1073/pnas.172501299 – ident: e_1_3_2_19_2 doi: 10.1128/jb.177.24.7011-7018.1995 – ident: e_1_3_2_27_2 doi: 10.1128/JB.183.17.5110-5121.2001 – ident: e_1_3_2_39_2 doi: 10.1007/s002840010156 – ident: e_1_3_2_52_2 doi: 10.1073/pnas.97.26.14668 – ident: e_1_3_2_51_2 doi: 10.1146/annurev.micro.55.1.709 – ident: e_1_3_2_4_2 doi: 10.1093/jn/125.6.1401 – ident: e_1_3_2_15_2 doi: 10.1016/S0022-2836(05)80360-2 – ident: e_1_3_2_20_2 doi: 10.1016/S0021-9258(18)42880-3 – ident: e_1_3_2_46_2 doi: 10.1128/iai.60.11.4621-4632.1992 – ident: e_1_3_2_16_2 doi: 10.1093/nar/22.22.4673 – ident: e_1_3_2_32_2 doi: 10.1128/JB.184.1.126-133.2002 – ident: e_1_3_2_30_2 doi: 10.1128/JB.181.9.2863-2871.1999 – ident: e_1_3_2_21_2 doi: 10.1006/jmbi.1999.2624 |
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Snippet | Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate... Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate... |
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SubjectTerms | ATP binding cassette transporters ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biological Sciences Biological Transport, Active Chromosome Mapping Comparative analysis DNA, Bacterial - genetics Enzymes Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Genes Genetic loci Genome, Bacterial Genomes Genomics Lac Repressors Lactobacillus acidophilus - genetics Lactobacillus acidophilus - metabolism Molecular Sequence Data Multigene Family Mutation Oligosaccharides - metabolism Operon Operons Phenotype Phylogeny Regulator genes Repression Repressor Proteins - genetics Repressor Proteins - metabolism RNA Sequence Homology, Nucleic Acid Species Specificity Sugars |
Title | Functional and Comparative Genomic Analyses of an Operon Involved in Fructooligosaccharide Utilization by Lactobacillus acidophilus |
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