In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity
According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herp...
Saved in:
Published in | Immunity (Cambridge, Mass.) Vol. 44; no. 2; pp. 233 - 245 |
---|---|
Main Authors | , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
16.02.2016
Elsevier Limited Cell Press |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2–16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8+ T cell immunity.
[Display omitted]
•Two-photon imaging indicates that CTLs kill 2–16 virus-infected cells per day•CTLs form kinapses rather than stable synapses when killing virus-infected cells•Some CTL contacts trigger long-lasting calcium fluxes in virus-infected cells•CTLs can cooperate during killing of virus-infected cells
According to in vitro assays, T cells are thought to kill rapidly and efficiently. Using two-photon microscopy, Forster and colleagues have found that killing capacities of single cytotoxic T lymphocytes (CTLs) in vivo are heterogeneous and limited. Quantification of target-cell-death probabilities identified efficient cooperative killing when multiple CTLs attacked a virus-infected cell. |
---|---|
AbstractList | According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2-16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8+T cell immunity. According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2-16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8(+) T cell immunity. According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2–16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8+ T cell immunity. [Display omitted] •Two-photon imaging indicates that CTLs kill 2–16 virus-infected cells per day•CTLs form kinapses rather than stable synapses when killing virus-infected cells•Some CTL contacts trigger long-lasting calcium fluxes in virus-infected cells•CTLs can cooperate during killing of virus-infected cells According to in vitro assays, T cells are thought to kill rapidly and efficiently. Using two-photon microscopy, Forster and colleagues have found that killing capacities of single cytotoxic T lymphocytes (CTLs) in vivo are heterogeneous and limited. Quantification of target-cell-death probabilities identified efficient cooperative killing when multiple CTLs attacked a virus-infected cell. According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2–16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8 + T cell immunity. • Two-photon imaging indicates that CTLs kill 2–16 virus-infected cells per day • CTLs form kinapses rather than stable synapses when killing virus-infected cells • Some CTL contacts trigger long-lasting calcium fluxes in virus-infected cells • CTLs can cooperate during killing of virus-infected cells According to in vitro assays, T cells are thought to kill rapidly and efficiently. Using two-photon microscopy, Forster and colleagues have found that killing capacities of single cytotoxic T lymphocytes (CTLs) in vivo are heterogeneous and limited. Quantification of target-cell-death probabilities identified efficient cooperative killing when multiple CTLs attacked a virus-infected cell. |
Author | Busche, Andreas Heller, Katrin Martens, Rieke Marquardt, Anja Zheng, Xiang Galla, Melanie Arens, Ramon Messerle, Martin Förster, Reinhold Stahl, Felix Rolf Sutter, Gerd Bischoff, Yvonne Uvarovskii, Alexey Meyer-Hermann, Michael Boelter, Jasmin Wagner, Karen Werth, Kathrin Halle, Stephan Heissmeyer, Vigo Braun, Asolina Kremer, Melanie Keyser, Kirsten Anja Kempf, Harald |
AuthorAffiliation | 2 Institute of Virology, Hannover Medical School, 30625 Hannover, Germany 3 Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany 1 Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany 8 Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands 5 Institute of Molecular Immunology, Helmholtz Zentrum München, 81377 München, Germany 9 Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-Universität München, 80539 München, Germany 4 Institute for Immunology, Ludwig-Maximilians-Universität München, 80336 München, Germany 6 Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany 7 Institute for Biochemistry, Biotechnology, and Bioinformatics, Technische Universität Braunschweig, 38124 Braunschweig, Germany |
AuthorAffiliation_xml | – name: 5 Institute of Molecular Immunology, Helmholtz Zentrum München, 81377 München, Germany – name: 3 Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany – name: 2 Institute of Virology, Hannover Medical School, 30625 Hannover, Germany – name: 1 Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – name: 6 Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany – name: 7 Institute for Biochemistry, Biotechnology, and Bioinformatics, Technische Universität Braunschweig, 38124 Braunschweig, Germany – name: 8 Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands – name: 9 Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-Universität München, 80539 München, Germany – name: 4 Institute for Immunology, Ludwig-Maximilians-Universität München, 80336 München, Germany |
Author_xml | – sequence: 1 givenname: Stephan surname: Halle fullname: Halle, Stephan email: halle.stephan@mh-hannover.de organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 2 givenname: Kirsten Anja surname: Keyser fullname: Keyser, Kirsten Anja organization: Institute of Virology, Hannover Medical School, 30625 Hannover, Germany – sequence: 3 givenname: Felix Rolf surname: Stahl fullname: Stahl, Felix Rolf organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 4 givenname: Andreas surname: Busche fullname: Busche, Andreas organization: Institute of Virology, Hannover Medical School, 30625 Hannover, Germany – sequence: 5 givenname: Anja surname: Marquardt fullname: Marquardt, Anja organization: Institute of Virology, Hannover Medical School, 30625 Hannover, Germany – sequence: 6 givenname: Xiang surname: Zheng fullname: Zheng, Xiang organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 7 givenname: Melanie surname: Galla fullname: Galla, Melanie organization: Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany – sequence: 8 givenname: Vigo surname: Heissmeyer fullname: Heissmeyer, Vigo organization: Institute for Immunology, Ludwig-Maximilians-Universität München, 80336 München, Germany – sequence: 9 givenname: Katrin surname: Heller fullname: Heller, Katrin organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 10 givenname: Jasmin surname: Boelter fullname: Boelter, Jasmin organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 11 givenname: Karen surname: Wagner fullname: Wagner, Karen organization: Institute of Virology, Hannover Medical School, 30625 Hannover, Germany – sequence: 12 givenname: Yvonne surname: Bischoff fullname: Bischoff, Yvonne organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 13 givenname: Rieke surname: Martens fullname: Martens, Rieke organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 14 givenname: Asolina surname: Braun fullname: Braun, Asolina organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 15 givenname: Kathrin surname: Werth fullname: Werth, Kathrin organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany – sequence: 16 givenname: Alexey surname: Uvarovskii fullname: Uvarovskii, Alexey organization: Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany – sequence: 17 givenname: Harald surname: Kempf fullname: Kempf, Harald organization: Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany – sequence: 18 givenname: Michael surname: Meyer-Hermann fullname: Meyer-Hermann, Michael organization: Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany – sequence: 19 givenname: Ramon surname: Arens fullname: Arens, Ramon organization: Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands – sequence: 20 givenname: Melanie surname: Kremer fullname: Kremer, Melanie organization: Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-Universität München, 80539 München, Germany – sequence: 21 givenname: Gerd surname: Sutter fullname: Sutter, Gerd organization: Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-Universität München, 80539 München, Germany – sequence: 22 givenname: Martin surname: Messerle fullname: Messerle, Martin organization: Institute of Virology, Hannover Medical School, 30625 Hannover, Germany – sequence: 23 givenname: Reinhold surname: Förster fullname: Förster, Reinhold email: foerster.reinhold@mh-hannover.de organization: Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26872694$$D View this record in MEDLINE/PubMed |
BookMark | eNqNkstu1DAUhi1URNuBN0DIEhs2mfoWO9kgoXCLOlI3ha3lcZziUWIPthN13oZn4clwmFIuiwrJ0rF1vvOfi885OHHeGQCeY7TGCPOL3dqO4-TsmuTXGuF80CNwhlEtCoYrdLLcBSsEx_QUnMe4QwizskZPwCnhlSC8Zmcgte77t8929vDSDoN1N7BRe6VtOkDfw-aQfPK3VsNr2JhhiLCNcGNHm0wHletg62Y_zCbCtwenxsy1LpmgdLLexZ_EMRA23u-zI9k5Sz8Fj3s1RPPszq7Ap_fvrpuPxebqQ9u82RS6JCwVJaNM96KvqkoJzbAmte57pSmqKcseTjnZUi0oFtuSdvVW9zWqiRKEiMXQFXh91N1P29F02rgU1CD3wY4qHKRXVv7tcfaLvPGzZBXjtaiywKs7geC_TiYmOdqocz_KGT9FiQWvypyLkv9BBeIVz7WvwMt_0J2fgsuTWCheipLjMlPsSOngYwymv68bI7lsgNzJ4wbIZQMkwvmgHPbiz57vg359-e-hmDz52Zogo7bGadPZYHSSnbcPZ_gBIYDGqQ |
CitedBy_id | crossref_primary_10_1083_jcb_201712085 crossref_primary_10_1158_2326_6066_CIR_18_0750 crossref_primary_10_7554_eLife_84916 crossref_primary_10_1038_s41586_022_04825_8 crossref_primary_10_1158_0008_5472_CAN_18_3712 crossref_primary_10_1007_s11538_018_0412_8 crossref_primary_10_1073_pnas_1710751114 crossref_primary_10_1371_journal_ppat_1008426 crossref_primary_10_1093_imammb_dqx009 crossref_primary_10_1016_j_eururo_2024_01_023 crossref_primary_10_3389_fimmu_2020_00400 crossref_primary_10_7554_eLife_58065 crossref_primary_10_1080_10428194_2016_1222381 crossref_primary_10_1016_j_it_2017_04_002 crossref_primary_10_1016_j_it_2016_04_001 crossref_primary_10_1038_s41467_022_30575_2 crossref_primary_10_1158_2326_6066_CIR_20_0741 crossref_primary_10_1111_imr_13037 crossref_primary_10_1186_s40425_019_0500_9 crossref_primary_10_1111_imr_13032 crossref_primary_10_1016_j_cobme_2017_03_002 crossref_primary_10_1002_wsbm_1510 crossref_primary_10_1016_j_trecan_2022_07_007 crossref_primary_10_1016_j_cell_2022_03_030 crossref_primary_10_1039_C9ME00036D crossref_primary_10_3389_fimmu_2024_1338218 crossref_primary_10_1007_s00430_019_00616_7 crossref_primary_10_1038_nrd_2016_233 crossref_primary_10_1172_jci_insight_167482 crossref_primary_10_3389_fphar_2016_00515 crossref_primary_10_15252_embj_2020106658 crossref_primary_10_1113_JP283667 crossref_primary_10_1016_j_it_2019_06_002 crossref_primary_10_1038_s41467_018_03662_6 crossref_primary_10_1038_s41467_021_25282_3 crossref_primary_10_1080_14760584_2018_1541407 crossref_primary_10_3389_fimmu_2022_849701 crossref_primary_10_1007_s00281_018_0714_9 crossref_primary_10_1007_s11538_022_01095_3 crossref_primary_10_1172_JCI132583 crossref_primary_10_1002_eji_202249921 crossref_primary_10_1016_j_nbd_2023_106308 crossref_primary_10_15252_embr_202357653 crossref_primary_10_1016_j_tcb_2023_02_008 crossref_primary_10_1021_acschembio_8b00155 crossref_primary_10_1038_s41598_019_53642_z crossref_primary_10_3389_fphar_2022_1056365 crossref_primary_10_3389_fimmu_2019_02153 crossref_primary_10_3389_fimmu_2018_00952 crossref_primary_10_1007_s00109_018_1628_7 crossref_primary_10_3389_fimmu_2022_894306 crossref_primary_10_1038_s41598_019_48711_2 crossref_primary_10_1073_pnas_2316500121 crossref_primary_10_1016_j_semcdb_2019_01_004 crossref_primary_10_1016_j_coi_2023_102307 crossref_primary_10_1128_jvi_00509_22 crossref_primary_10_1371_journal_ppat_1005895 crossref_primary_10_3389_fphys_2020_590479 crossref_primary_10_1242_dmm_034462 crossref_primary_10_1098_rspb_2023_2280 crossref_primary_10_1002_acn3_783 crossref_primary_10_1007_s13402_021_00601_4 crossref_primary_10_1038_s41586_018_0812_9 crossref_primary_10_1016_j_ccell_2017_04_003 crossref_primary_10_3390_v15071454 crossref_primary_10_1016_j_copbio_2020_12_024 crossref_primary_10_1126_sciimmunol_aaz4371 crossref_primary_10_3389_fimmu_2019_00360 crossref_primary_10_1096_fj_202200033R crossref_primary_10_1128_JVI_00095_17 crossref_primary_10_18632_aging_203113 crossref_primary_10_1007_s00285_024_02065_0 crossref_primary_10_1016_j_coviro_2021_02_001 crossref_primary_10_1111_imr_12433 crossref_primary_10_1371_journal_pntd_0007547 crossref_primary_10_1093_brain_awaa269 crossref_primary_10_1016_j_celrep_2018_03_072 crossref_primary_10_1111_imr_12672 crossref_primary_10_3389_fimmu_2022_931820 crossref_primary_10_1016_j_it_2021_06_004 crossref_primary_10_1016_j_celrep_2023_112597 crossref_primary_10_1038_s41598_020_64408_3 crossref_primary_10_3390_v13081563 crossref_primary_10_1371_journal_ppat_1007252 crossref_primary_10_1002_psp4_12925 crossref_primary_10_1002_psp4_12800 crossref_primary_10_3389_fimmu_2022_896228 crossref_primary_10_3389_fimmu_2018_01949 crossref_primary_10_1084_jem_20210314 crossref_primary_10_1016_j_ymthe_2017_06_003 crossref_primary_10_1172_jci_insight_93684 crossref_primary_10_1172_jci_insight_168110 crossref_primary_10_1371_journal_pcbi_1007972 crossref_primary_10_7554_eLife_68864 crossref_primary_10_1016_j_bpj_2017_02_008 crossref_primary_10_1093_brain_aww225 crossref_primary_10_1126_sciadv_aay6298 crossref_primary_10_1002_1878_0261_13582 crossref_primary_10_1016_j_ceca_2016_12_007 crossref_primary_10_1016_j_celrep_2017_12_052 crossref_primary_10_4049_jimmunol_1900232 crossref_primary_10_15252_embj_2018100928 crossref_primary_10_1007_s11904_020_00481_7 crossref_primary_10_1371_journal_pcbi_1009735 crossref_primary_10_3389_fonc_2021_653625 crossref_primary_10_3390_ijms20163865 crossref_primary_10_1016_j_immuni_2020_04_022 crossref_primary_10_3390_v10120693 crossref_primary_10_1128_mSphere_00381_18 crossref_primary_10_1172_JCI131696 crossref_primary_10_1021_acs_chemrev_1c00666 crossref_primary_10_1002_cyto_a_23938 crossref_primary_10_3389_fimmu_2016_00428 crossref_primary_10_3389_fimmu_2016_00668 crossref_primary_10_1084_jem_20182375 crossref_primary_10_1126_science_aay9207 crossref_primary_10_1038_cddis_2017_67 crossref_primary_10_1128_JVI_00306_16 crossref_primary_10_1371_journal_ppat_1009255 crossref_primary_10_1126_science_abl3855 crossref_primary_10_3389_fimmu_2021_704862 crossref_primary_10_1016_j_cellsig_2020_109772 crossref_primary_10_1083_jcb_202104093 crossref_primary_10_1016_j_celrep_2016_07_037 crossref_primary_10_1038_cti_2016_74 crossref_primary_10_1371_journal_pcbi_1007626 crossref_primary_10_1080_2162402X_2017_1306619 crossref_primary_10_1136_jitc_2020_001877 crossref_primary_10_1038_s41598_018_36055_2 crossref_primary_10_1371_journal_ppat_1009818 crossref_primary_10_1109_JBHI_2020_3003475 crossref_primary_10_1126_sciimmunol_add5724 crossref_primary_10_1038_s41467_017_01032_2 crossref_primary_10_1089_vim_2019_0173 crossref_primary_10_1016_j_immuni_2016_01_026 crossref_primary_10_1088_1361_6560_ac176b crossref_primary_10_3390_ijms23073578 crossref_primary_10_4049_jimmunol_1800471 crossref_primary_10_1016_j_coviro_2016_11_010 crossref_primary_10_1117_1_JBO_24_5_051413 crossref_primary_10_3390_ijms232214122 crossref_primary_10_3389_fimmu_2021_626019 crossref_primary_10_1158_0008_5472_CAN_18_3147 crossref_primary_10_1038_s41586_019_1054_1 crossref_primary_10_1038_s41587_022_01397_w crossref_primary_10_1007_s00262_019_02361_5 crossref_primary_10_1371_journal_pcbi_1008428 crossref_primary_10_1126_sciimmunol_aaq0491 crossref_primary_10_1016_j_xcrm_2021_100317 crossref_primary_10_7554_eLife_62691 crossref_primary_10_1155_2020_4106518 crossref_primary_10_1016_j_molimm_2019_03_010 crossref_primary_10_1016_j_isci_2022_104395 crossref_primary_10_1016_j_yexcr_2020_112014 crossref_primary_10_1016_j_biopha_2019_109626 crossref_primary_10_1002_wsbm_1446 |
Cites_doi | 10.1016/j.it.2012.04.001 10.1126/science.1221063 10.1016/j.immuni.2006.04.015 10.1038/sj.icb.7100053 10.1172/JCI58653 10.1172/JCI34388 10.1073/pnas.0600651103 10.4049/jimmunol.181.6.3818 10.1128/JVI.01128-08 10.1126/science.1260044 10.1016/S1074-7613(00)80242-3 10.1084/jem.190.9.1285 10.1038/ni.2050 10.1016/j.immuni.2012.11.012 10.1007/978-1-61779-876-4_4 10.1016/j.coi.2010.04.002 10.1126/science.274.5284.94 10.1093/cid/cit048 10.1038/nri2638 10.1038/ni.2085 10.1016/j.immuni.2009.05.009 10.1016/S1097-2765(00)80063-5 10.1016/j.immuni.2011.07.010 10.1016/j.chom.2013.01.004 10.1126/science.1185350 10.1099/vir.0.029827-0 10.1111/j.1600-065X.2008.00589.x 10.1038/nature12354 10.1371/journal.pcbi.1004178 10.1016/j.immuni.2012.09.012 10.1016/j.immuni.2012.01.002 10.1111/imr.12015 10.1073/pnas.1303858110 10.1016/j.immuni.2015.02.009 10.1016/j.immuni.2011.05.017 10.1016/j.immuni.2015.04.013 10.1038/nature02238 10.4049/jimmunol.172.11.6944 10.1016/j.immuni.2005.08.001 10.1371/journal.pcbi.1002381 10.1371/journal.pcbi.1003534 10.1084/jem.20091472 10.1371/journal.pcbi.1003805 |
ContentType | Journal Article |
Copyright | 2016 Elsevier Inc. Copyright © 2016 Elsevier Inc. All rights reserved. Copyright Elsevier Limited Feb 16, 2016 2016 The Authors. Published by Elsevier Inc. 2016 Elsevier Inc. |
Copyright_xml | – notice: 2016 Elsevier Inc. – notice: Copyright © 2016 Elsevier Inc. All rights reserved. – notice: Copyright Elsevier Limited Feb 16, 2016 – notice: 2016 The Authors. Published by Elsevier Inc. 2016 Elsevier Inc. |
DBID | 6I. AAFTH CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QL 7QP 7QR 7T5 7T7 7TK 7TM 7U9 8FD C1K FR3 H94 K9. M7N NAPCQ P64 RC3 7X8 5PM |
DOI | 10.1016/j.immuni.2016.01.010 |
DatabaseName | ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Immunology Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Neurosciences Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Algology Mycology and Protozoology Abstracts (Microbiology C) Nursing & Allied Health Premium Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Technology Research Database Nucleic Acids Abstracts ProQuest Health & Medical Complete (Alumni) Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Nursing & Allied Health Premium Genetics Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Immunology Abstracts Engineering Research Database Industrial and Applied Microbiology Abstracts (Microbiology A) Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
DatabaseTitleList | Virology and AIDS Abstracts MEDLINE - Academic MEDLINE AIDS and Cancer Research Abstracts |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Biology |
EISSN | 1097-4180 |
EndPage | 245 |
ExternalDocumentID | 3957550371 10_1016_j_immuni_2016_01_010 26872694 S1074761316000352 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K -DZ 0R~ 1RT 1~5 2WC 3V. 4.4 457 4G. 53G 5GY 62- 6I. 7-5 7RV 7X7 8C1 8FE 8FH AACTN AAEDT AAEDW AAFTH AAIAV AAKRW AAUCE AAVLU AAXJY AAXUO ABMAC ABMWF ABOCM ABVKL ACGFO ACGFS ACIWK ACPRK ADBBV ADEZE ADFRT ADJPV AEFWE AENEX AEXQZ AFKRA AFRAH AFTJW AGGSO AGKMS AHHHB AHMBA AITUG ALKID ALMA_UNASSIGNED_HOLDINGS AMRAJ ASPBG AVWKF AZFZN BAWUL BBNVY BENPR BHPHI BKEYQ BPHCQ BVXVI C45 CS3 DIK DU5 E3Z EBS EJD F5P FCP FDB FIRID HCIFZ IH2 IHE IXB J1W JIG LK8 LX5 M2O M3Z M41 M7P N9A NCXOZ O-L O9- OK1 OVD P2P PQQKQ PROAC RCE RIG ROL RPZ SCP SES SSZ TEORI TR2 WQ6 ZA5 0SF AKAPO AKRWK CGR CUY CVF ECM EIF NPM .55 .GJ 29I 5VS AAIKJ AALRI AAQFI AAQXK AAYXX ADMUD ADVLN AGHFR CITATION FEDTE FGOYB G-2 HVGLF HZ~ OHT OZT R2- UHS X7M Y6R ZGI 7QL 7QP 7QR 7T5 7T7 7TK 7TM 7U9 8FD AAMRU C1K FR3 H94 K9. M7N NAPCQ P64 RC3 7X8 5PM |
ID | FETCH-LOGICAL-c524t-5434cf7f888a7c41c29cffac309344cf6362b3c7317b53d9bcf9092a722792a73 |
IEDL.DBID | IXB |
ISSN | 1074-7613 |
IngestDate | Tue Sep 17 21:17:56 EDT 2024 Fri Aug 16 06:14:50 EDT 2024 Fri Aug 16 04:46:21 EDT 2024 Wed Sep 25 02:34:06 EDT 2024 Fri Aug 23 01:56:07 EDT 2024 Thu May 23 23:21:42 EDT 2024 Fri Feb 23 02:28:38 EST 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
License | http://creativecommons.org/licenses/by-nc-nd/4.0 Copyright © 2016 Elsevier Inc. All rights reserved. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c524t-5434cf7f888a7c41c29cffac309344cf6362b3c7317b53d9bcf9092a722792a73 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Institute of Microbiology and Immunology, University of Melbourne, Melbourne, VIC 3010, Australia Present address: Institute of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany Present address: Octapharma Produktionsgesellschaft Deutschland mbH, 31832 Springe, Germany Present address: Merck Animal Health, Burgwedel Biotech GmbH, 30938 Burgwedel, Germany |
OpenAccessLink | https://www.sciencedirect.com/science/article/pii/S1074761316000352 |
PMID | 26872694 |
PQID | 1766575615 |
PQPubID | 2031079 |
PageCount | 13 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_4846978 proquest_miscellaneous_1768579232 proquest_miscellaneous_1767068609 proquest_journals_1766575615 crossref_primary_10_1016_j_immuni_2016_01_010 pubmed_primary_26872694 elsevier_sciencedirect_doi_10_1016_j_immuni_2016_01_010 |
PublicationCentury | 2000 |
PublicationDate | 2016-02-16 |
PublicationDateYYYYMMDD | 2016-02-16 |
PublicationDate_xml | – month: 02 year: 2016 text: 2016-02-16 day: 16 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States – name: Cambridge |
PublicationTitle | Immunity (Cambridge, Mass.) |
PublicationTitleAlternate | Immunity |
PublicationYear | 2016 |
Publisher | Elsevier Inc Elsevier Limited Cell Press |
Publisher_xml | – name: Elsevier Inc – name: Elsevier Limited – name: Cell Press |
References | Krmpotic, Messerle, Crnkovic-Mertens, Polic, Jonjic, Koszinowski (bib25) 1999; 190 Plotkin (bib34) 2013; 56 Hogan, Kadolsky, Tung, Seddon, Yates (bib20) 2014; 10 Jenkins, Mintern, La Gruta, Kedzierska, Doherty, Turner (bib21) 2008; 181 Lopez, Brennan, Whisstock, Voskoboinik, Trapani (bib27) 2012; 33 Keefe, Shi, Feske, Massol, Navarro, Kirchhausen, Lieberman (bib23) 2005; 23 Cockburn, Amino, Kelemen, Kuo, Tse, Radtke, Mac-Daniel, Ganusov, Zavala, Ménard (bib7) 2013; 110 Liu, Bryceson, Meckel, Vasiliver-Shamis, Dustin, Long (bib26) 2009; 31 Breart, Lemaître, Celli, Bousso (bib5) 2008; 118 Hickman, Reynoso, Ngudiankama, Cush, Gibbs, Bennink, Yewdell (bib19) 2015; 42 Altman, Moss, Goulder, Barouch, McHeyzer-Williams, Bell, McMichael, Davis (bib1) 1996; 274 Newell, Sigal, Bendall, Nolan, Davis (bib33) 2012; 36 Thiery, Keefe, Boulant, Boucrot, Walch, Martinvalet, Goping, Bleackley, Kirchhausen, Lieberman (bib37) 2011; 12 Varadarajan, Julg, Yamanaka, Chen, Ogunniyi, McAndrew, Porter, Piechocka-Trocha, Hill, Douek (bib38) 2011; 121 Halle, Dujardin, Bakocevic, Fleige, Danzer, Willenzon, Suezer, Hämmerling, Garbi, Sutter (bib16) 2009; 206 Marangoni, Murooka, Manzo, Kim, Carrizosa, Elpek, Mempel (bib28) 2013; 38 Regoes, Yates, Antia (bib35) 2007; 85 Mempel, Pittet, Khazaie, Weninger, Weissleder, von Boehmer, von Andrian (bib32) 2006; 25 Dustin (bib9) 2008; 221 Ziegler, Thale, Lucin, Muranyi, Flohr, Hengel, Farrell, Rawlinson, Koszinowski (bib43) 1997; 6 Garcia, Richter, Graw, Oxenius, Regoes (bib13) 2015; 11 Elemans, Seich Al Basatena, Asquith (bib10) 2012; 8 Zhang, Bevan (bib42) 2011; 35 Hickman, Reynoso, Ngudiankama, Rubin, Magadán, Cush, Gibbs, Molon, Bronte, Bennink, Yewdell (bib18) 2013; 13 Aramburu, Garcia-Cózar, Raghavan, Okamura, Rao, Hogan (bib2) 1998; 1 Marquardt, Halle, Seckert, Lemmermann, Veres, Braun, Maus, Förster, Reddehase, Messerle, Busche (bib30) 2011; 92 Elemans, Florins, Willems, Asquith (bib11) 2014; 10 Beltman, Marée, de Boer (bib3) 2009; 9 Ritter, Asano, Stinchcombe, Dieckmann, Chen, Gawden-Bone, van Engelenburg, Legant, Gao, Davidson (bib36) 2015; 42 Wiedemann, Depoil, Faroudi, Valitutti (bib40) 2006; 103 Deguine, Bousso (bib8) 2013; 251 Braun, Worbs, Moschovakis, Halle, Hoffmann, Bölter, Münk, Förster (bib4) 2011; 12 Germain, Robey, Cahalan (bib14) 2012; 336 Kastenmüller, Brandes, Wang, Herz, Egen, Germain (bib22) 2013; 38 Hansen, Powers, Richards, Ventura, Ford, Siess, Axthelm, Nelson, Jarvis, Picker, Früh (bib17) 2010; 328 Marchingo, Kan, Sutherland, Duffy, Wellard, Belz, Lew, Dowling, Heinzel, Hodgkin (bib29) 2014; 346 Mempel, Henrickson, Von Andrian (bib31) 2004; 427 West, Youngblood, Tan, Jin, Araki, Alexe, Konieczny, Calpe, Freeman, Terhorst (bib39) 2011; 35 Ganusov, De Boer (bib12) 2008; 82 Chen, Wardill, Sun, Pulver, Renninger, Baohan, Schreiter, Kerr, Orger, Jayaraman (bib6) 2013; 499 Kremer, Volz, Kreijtz, Fux, Lehmann, Sutter (bib24) 2012; 890 Yewdell (bib41) 2010; 22 Gold, Munks, Wagner, McMahon, Kelly, Kavanagh, Slifka, Koszinowski, Raulet, Hill (bib15) 2004; 172 22688761 - Methods Mol Biol. 2012;890:59-92 22745423 - Science. 2012 Jun 29;336(6089):1676-81 20360110 - Science. 2010 Apr 2;328(5974):102-6 21965332 - J Clin Invest. 2011 Nov;121(11):4322-31 19592272 - Immunity. 2009 Jul 17;31(1):99-109 8810254 - Science. 1996 Oct 4;274(5284):94-6 25430770 - Science. 2014 Nov 28;346(6213):1123-7 25933039 - PLoS Comput Biol. 2015 May;11(5):e1004178 18768835 - J Immunol. 2008 Sep 15;181(6):3818-22 19834485 - Nat Rev Immunol. 2009 Nov;9(11):789-98 14712275 - Nature. 2004 Jan 8;427(6970):154-9 23352234 - Immunity. 2013 Mar 21;38(3):502-13 23414756 - Cell Host Microbe. 2013 Feb 13;13(2):155-68 16860762 - Immunity. 2006 Jul;25(1):129-41 25992860 - Immunity. 2015 May 19;42(5):864-76 16832064 - Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):10985-90 23386629 - Clin Infect Dis. 2013 May;56(10):1458-65 23278747 - Immunol Rev. 2013 Jan;251(1):154-9 20447814 - Curr Opin Immunol. 2010 Jun;22(3):402-10 26885849 - Immunity. 2016 Feb 16;44(2):207-8 22608996 - Trends Immunol. 2012 Aug;33(8):406-12 25233372 - PLoS Comput Biol. 2014 Sep;10(9):e1003805 21325477 - J Gen Virol. 2011 Jun;92(Pt 6):1279-91 15153514 - J Immunol. 2004 Jun 1;172(11):6944-53 23868258 - Nature. 2013 Jul 18;499(7458):295-300 22383867 - PLoS Comput Biol. 2012;8(2):e1002381 23313588 - Immunity. 2013 Feb 21;38(2):237-49 16169498 - Immunity. 2005 Sep;23(3):249-62 9052837 - Immunity. 1997 Jan;6(1):57-66 10544200 - J Exp Med. 1999 Nov 1;190(9):1285-96 21841786 - Nat Immunol. 2011 Sep;12(9):879-87 18275476 - Immunol Rev. 2008 Feb;221:77-89 9660947 - Mol Cell. 1998 Apr;1(5):627-37 17420769 - Immunol Cell Biol. 2007 Jun;85(4):274-9 18357341 - J Clin Invest. 2008 Apr;118(4):1390-7 21867926 - Immunity. 2011 Aug 26;35(2):161-8 21856186 - Immunity. 2011 Aug 26;35(2):285-98 19917776 - J Exp Med. 2009 Nov 23;206(12):2593-601 25769612 - Immunity. 2015 Mar 17;42(3):524-37 21685908 - Nat Immunol. 2011 Aug;12(8):770-7 18815293 - J Virol. 2008 Dec;82(23):11749-57 23674673 - Proc Natl Acad Sci U S A. 2013 May 28;110(22):9090-5 24699260 - PLoS Comput Biol. 2014 Apr;10(4):e1003534 22265676 - Immunity. 2012 Jan 27;36(1):142-52 Beltman (10.1016/j.immuni.2016.01.010_bib3) 2009; 9 Elemans (10.1016/j.immuni.2016.01.010_bib11) 2014; 10 Varadarajan (10.1016/j.immuni.2016.01.010_bib38) 2011; 121 Kremer (10.1016/j.immuni.2016.01.010_bib24) 2012; 890 Ganusov (10.1016/j.immuni.2016.01.010_bib12) 2008; 82 Zhang (10.1016/j.immuni.2016.01.010_bib42) 2011; 35 Marangoni (10.1016/j.immuni.2016.01.010_bib28) 2013; 38 Hickman (10.1016/j.immuni.2016.01.010_bib19) 2015; 42 Ritter (10.1016/j.immuni.2016.01.010_bib36) 2015; 42 Garcia (10.1016/j.immuni.2016.01.010_bib13) 2015; 11 Gold (10.1016/j.immuni.2016.01.010_bib15) 2004; 172 Dustin (10.1016/j.immuni.2016.01.010_bib9) 2008; 221 Hansen (10.1016/j.immuni.2016.01.010_bib17) 2010; 328 Hickman (10.1016/j.immuni.2016.01.010_bib18) 2013; 13 Mempel (10.1016/j.immuni.2016.01.010_bib32) 2006; 25 Regoes (10.1016/j.immuni.2016.01.010_bib35) 2007; 85 Halle (10.1016/j.immuni.2016.01.010_bib16) 2009; 206 Ziegler (10.1016/j.immuni.2016.01.010_bib43) 1997; 6 Kastenmüller (10.1016/j.immuni.2016.01.010_bib22) 2013; 38 Deguine (10.1016/j.immuni.2016.01.010_bib8) 2013; 251 Aramburu (10.1016/j.immuni.2016.01.010_bib2) 1998; 1 Krmpotic (10.1016/j.immuni.2016.01.010_bib25) 1999; 190 Jenkins (10.1016/j.immuni.2016.01.010_bib21) 2008; 181 Keefe (10.1016/j.immuni.2016.01.010_bib23) 2005; 23 Germain (10.1016/j.immuni.2016.01.010_bib14) 2012; 336 Yewdell (10.1016/j.immuni.2016.01.010_bib41) 2010; 22 Braun (10.1016/j.immuni.2016.01.010_bib4) 2011; 12 Hogan (10.1016/j.immuni.2016.01.010_bib20) 2014; 10 Marquardt (10.1016/j.immuni.2016.01.010_bib30) 2011; 92 Wiedemann (10.1016/j.immuni.2016.01.010_bib40) 2006; 103 Altman (10.1016/j.immuni.2016.01.010_bib1) 1996; 274 Lopez (10.1016/j.immuni.2016.01.010_bib27) 2012; 33 Elemans (10.1016/j.immuni.2016.01.010_bib10) 2012; 8 West (10.1016/j.immuni.2016.01.010_bib39) 2011; 35 Breart (10.1016/j.immuni.2016.01.010_bib5) 2008; 118 Plotkin (10.1016/j.immuni.2016.01.010_bib34) 2013; 56 Newell (10.1016/j.immuni.2016.01.010_bib33) 2012; 36 Marchingo (10.1016/j.immuni.2016.01.010_bib29) 2014; 346 Liu (10.1016/j.immuni.2016.01.010_bib26) 2009; 31 Thiery (10.1016/j.immuni.2016.01.010_bib37) 2011; 12 Cockburn (10.1016/j.immuni.2016.01.010_bib7) 2013; 110 Mempel (10.1016/j.immuni.2016.01.010_bib31) 2004; 427 Chen (10.1016/j.immuni.2016.01.010_bib6) 2013; 499 |
References_xml | – volume: 427 start-page: 154 year: 2004 end-page: 159 ident: bib31 article-title: T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases publication-title: Nature contributor: fullname: Von Andrian – volume: 499 start-page: 295 year: 2013 end-page: 300 ident: bib6 article-title: Ultrasensitive fluorescent proteins for imaging neuronal activity publication-title: Nature contributor: fullname: Jayaraman – volume: 103 start-page: 10985 year: 2006 end-page: 10990 ident: bib40 article-title: Cytotoxic T lymphocytes kill multiple targets simultaneously via spatiotemporal uncoupling of lytic and stimulatory synapses publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Valitutti – volume: 8 start-page: e1002381 year: 2012 ident: bib10 article-title: The efficiency of the human CD8+ T cell response: how should we quantify it, what determines it, and does it matter? publication-title: PLoS Comput. Biol. contributor: fullname: Asquith – volume: 42 start-page: 864 year: 2015 end-page: 876 ident: bib36 article-title: Actin depletion initiates events leading to granule secretion at the immunological synapse publication-title: Immunity contributor: fullname: Davidson – volume: 10 start-page: e1003805 year: 2014 ident: bib20 article-title: Spatial heterogeneity and peptide availability determine CTL killing efficiency in vivo publication-title: PLoS Comput. Biol. contributor: fullname: Yates – volume: 12 start-page: 879 year: 2011 end-page: 887 ident: bib4 article-title: Afferent lymph-derived T cells and DCs use different chemokine receptor CCR7-dependent routes for entry into the lymph node and intranodal migration publication-title: Nat. Immunol. contributor: fullname: Förster – volume: 85 start-page: 274 year: 2007 end-page: 279 ident: bib35 article-title: Mathematical models of cytotoxic T-lymphocyte killing publication-title: Immunol. Cell Biol. contributor: fullname: Antia – volume: 1 start-page: 627 year: 1998 end-page: 637 ident: bib2 article-title: Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT publication-title: Mol. Cell contributor: fullname: Hogan – volume: 31 start-page: 99 year: 2009 end-page: 109 ident: bib26 article-title: Integrin-dependent organization and bidirectional vesicular traffic at cytotoxic immune synapses publication-title: Immunity contributor: fullname: Long – volume: 36 start-page: 142 year: 2012 end-page: 152 ident: bib33 article-title: Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes publication-title: Immunity contributor: fullname: Davis – volume: 13 start-page: 155 year: 2013 end-page: 168 ident: bib18 article-title: Anatomically restricted synergistic antiviral activities of innate and adaptive immune cells in the skin publication-title: Cell Host Microbe contributor: fullname: Yewdell – volume: 22 start-page: 402 year: 2010 end-page: 410 ident: bib41 article-title: Designing CD8+ T cell vaccines: it’s not rocket science (yet) publication-title: Curr. Opin. Immunol. contributor: fullname: Yewdell – volume: 6 start-page: 57 year: 1997 end-page: 66 ident: bib43 article-title: A mouse cytomegalovirus glycoprotein retains MHC class I complexes in the ERGIC/cis-Golgi compartments publication-title: Immunity contributor: fullname: Koszinowski – volume: 23 start-page: 249 year: 2005 end-page: 262 ident: bib23 article-title: Perforin triggers a plasma membrane-repair response that facilitates CTL induction of apoptosis publication-title: Immunity contributor: fullname: Lieberman – volume: 25 start-page: 129 year: 2006 end-page: 141 ident: bib32 article-title: Regulatory T cells reversibly suppress cytotoxic T cell function independent of effector differentiation publication-title: Immunity contributor: fullname: von Andrian – volume: 172 start-page: 6944 year: 2004 end-page: 6953 ident: bib15 article-title: Murine cytomegalovirus interference with antigen presentation has little effect on the size or the effector memory phenotype of the CD8 T cell response publication-title: J. Immunol. contributor: fullname: Hill – volume: 251 start-page: 154 year: 2013 end-page: 159 ident: bib8 article-title: Dynamics of NK cell interactions in vivo publication-title: Immunol. Rev. contributor: fullname: Bousso – volume: 12 start-page: 770 year: 2011 end-page: 777 ident: bib37 article-title: Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells publication-title: Nat. Immunol. contributor: fullname: Lieberman – volume: 33 start-page: 406 year: 2012 end-page: 412 ident: bib27 article-title: Protecting a serial killer: pathways for perforin trafficking and self-defence ensure sequential target cell death publication-title: Trends Immunol. contributor: fullname: Trapani – volume: 92 start-page: 1279 year: 2011 end-page: 1291 ident: bib30 article-title: Single cell detection of latent cytomegalovirus reactivation in host tissue publication-title: J. Gen. Virol. contributor: fullname: Busche – volume: 121 start-page: 4322 year: 2011 end-page: 4331 ident: bib38 article-title: A high-throughput single-cell analysis of human CD8 publication-title: J. Clin. Invest. contributor: fullname: Douek – volume: 38 start-page: 237 year: 2013 end-page: 249 ident: bib28 article-title: The transcription factor NFAT exhibits signal memory during serial T cell interactions with antigen-presenting cells publication-title: Immunity contributor: fullname: Mempel – volume: 11 start-page: e1004178 year: 2015 ident: bib13 article-title: Estimating the In Vivo Killing Efficacy of Cytotoxic T Lymphocytes across Different Peptide-MHC Complex Densities publication-title: PLoS Comput. Biol. contributor: fullname: Regoes – volume: 190 start-page: 1285 year: 1999 end-page: 1296 ident: bib25 article-title: The immunoevasive function encoded by the mouse cytomegalovirus gene m152 protects the virus against T cell control in vivo publication-title: J. Exp. Med. contributor: fullname: Koszinowski – volume: 35 start-page: 285 year: 2011 end-page: 298 ident: bib39 article-title: Tight regulation of memory CD8(+) T cells limits their effectiveness during sustained high viral load publication-title: Immunity contributor: fullname: Terhorst – volume: 206 start-page: 2593 year: 2009 end-page: 2601 ident: bib16 article-title: Induced bronchus-associated lymphoid tissue serves as a general priming site for T cells and is maintained by dendritic cells publication-title: J. Exp. Med. contributor: fullname: Sutter – volume: 274 start-page: 94 year: 1996 end-page: 96 ident: bib1 article-title: Phenotypic analysis of antigen-specific T lymphocytes publication-title: Science contributor: fullname: Davis – volume: 110 start-page: 9090 year: 2013 end-page: 9095 ident: bib7 article-title: In vivo imaging of CD8+ T cell-mediated elimination of malaria liver stages publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Ménard – volume: 56 start-page: 1458 year: 2013 end-page: 1465 ident: bib34 article-title: Complex correlates of protection after vaccination publication-title: Clin. Infect. Dis. contributor: fullname: Plotkin – volume: 10 start-page: e1003534 year: 2014 ident: bib11 article-title: Rates of CTL killing in persistent viral infection in vivo publication-title: PLoS Comput. Biol. contributor: fullname: Asquith – volume: 328 start-page: 102 year: 2010 end-page: 106 ident: bib17 article-title: Evasion of CD8+ T cells is critical for superinfection by cytomegalovirus publication-title: Science contributor: fullname: Früh – volume: 221 start-page: 77 year: 2008 end-page: 89 ident: bib9 article-title: T-cell activation through immunological synapses and kinapses publication-title: Immunol. Rev. contributor: fullname: Dustin – volume: 9 start-page: 789 year: 2009 end-page: 798 ident: bib3 article-title: Analysing immune cell migration publication-title: Nat. Rev. Immunol. contributor: fullname: de Boer – volume: 181 start-page: 3818 year: 2008 end-page: 3822 ident: bib21 article-title: Cell cycle-related acquisition of cytotoxic mediators defines the progressive differentiation to effector status for virus-specific CD8+ T cells publication-title: J. Immunol. contributor: fullname: Turner – volume: 38 start-page: 502 year: 2013 end-page: 513 ident: bib22 article-title: Peripheral prepositioning and local CXCL9 chemokine-mediated guidance orchestrate rapid memory CD8+ T cell responses in the lymph node publication-title: Immunity contributor: fullname: Germain – volume: 35 start-page: 161 year: 2011 end-page: 168 ident: bib42 article-title: CD8(+) T cells: foot soldiers of the immune system publication-title: Immunity contributor: fullname: Bevan – volume: 82 start-page: 11749 year: 2008 end-page: 11757 ident: bib12 article-title: Estimating in vivo death rates of targets due to CD8 T-cell-mediated killing publication-title: J. Virol. contributor: fullname: De Boer – volume: 42 start-page: 524 year: 2015 end-page: 537 ident: bib19 article-title: CXCR3 chemokine receptor enables local CD8(+) T cell migration for the destruction of virus-infected cells publication-title: Immunity contributor: fullname: Yewdell – volume: 346 start-page: 1123 year: 2014 end-page: 1127 ident: bib29 article-title: T cell signaling. Antigen affinity, costimulation, and cytokine inputs sum linearly to amplify T cell expansion publication-title: Science contributor: fullname: Hodgkin – volume: 118 start-page: 1390 year: 2008 end-page: 1397 ident: bib5 article-title: Two-photon imaging of intratumoral CD8+ T cell cytotoxic activity during adoptive T cell therapy in mice publication-title: J. Clin. Invest. contributor: fullname: Bousso – volume: 890 start-page: 59 year: 2012 end-page: 92 ident: bib24 article-title: Easy and efficient protocols for working with recombinant vaccinia virus MVA publication-title: Methods Mol. Biol. contributor: fullname: Sutter – volume: 336 start-page: 1676 year: 2012 end-page: 1681 ident: bib14 article-title: A decade of imaging cellular motility and interaction dynamics in the immune system publication-title: Science contributor: fullname: Cahalan – volume: 33 start-page: 406 year: 2012 ident: 10.1016/j.immuni.2016.01.010_bib27 article-title: Protecting a serial killer: pathways for perforin trafficking and self-defence ensure sequential target cell death publication-title: Trends Immunol. doi: 10.1016/j.it.2012.04.001 contributor: fullname: Lopez – volume: 336 start-page: 1676 year: 2012 ident: 10.1016/j.immuni.2016.01.010_bib14 article-title: A decade of imaging cellular motility and interaction dynamics in the immune system publication-title: Science doi: 10.1126/science.1221063 contributor: fullname: Germain – volume: 25 start-page: 129 year: 2006 ident: 10.1016/j.immuni.2016.01.010_bib32 article-title: Regulatory T cells reversibly suppress cytotoxic T cell function independent of effector differentiation publication-title: Immunity doi: 10.1016/j.immuni.2006.04.015 contributor: fullname: Mempel – volume: 85 start-page: 274 year: 2007 ident: 10.1016/j.immuni.2016.01.010_bib35 article-title: Mathematical models of cytotoxic T-lymphocyte killing publication-title: Immunol. Cell Biol. doi: 10.1038/sj.icb.7100053 contributor: fullname: Regoes – volume: 121 start-page: 4322 year: 2011 ident: 10.1016/j.immuni.2016.01.010_bib38 article-title: A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis publication-title: J. Clin. Invest. doi: 10.1172/JCI58653 contributor: fullname: Varadarajan – volume: 118 start-page: 1390 year: 2008 ident: 10.1016/j.immuni.2016.01.010_bib5 article-title: Two-photon imaging of intratumoral CD8+ T cell cytotoxic activity during adoptive T cell therapy in mice publication-title: J. Clin. Invest. doi: 10.1172/JCI34388 contributor: fullname: Breart – volume: 103 start-page: 10985 year: 2006 ident: 10.1016/j.immuni.2016.01.010_bib40 article-title: Cytotoxic T lymphocytes kill multiple targets simultaneously via spatiotemporal uncoupling of lytic and stimulatory synapses publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.0600651103 contributor: fullname: Wiedemann – volume: 181 start-page: 3818 year: 2008 ident: 10.1016/j.immuni.2016.01.010_bib21 article-title: Cell cycle-related acquisition of cytotoxic mediators defines the progressive differentiation to effector status for virus-specific CD8+ T cells publication-title: J. Immunol. doi: 10.4049/jimmunol.181.6.3818 contributor: fullname: Jenkins – volume: 82 start-page: 11749 year: 2008 ident: 10.1016/j.immuni.2016.01.010_bib12 article-title: Estimating in vivo death rates of targets due to CD8 T-cell-mediated killing publication-title: J. Virol. doi: 10.1128/JVI.01128-08 contributor: fullname: Ganusov – volume: 346 start-page: 1123 year: 2014 ident: 10.1016/j.immuni.2016.01.010_bib29 article-title: T cell signaling. Antigen affinity, costimulation, and cytokine inputs sum linearly to amplify T cell expansion publication-title: Science doi: 10.1126/science.1260044 contributor: fullname: Marchingo – volume: 6 start-page: 57 year: 1997 ident: 10.1016/j.immuni.2016.01.010_bib43 article-title: A mouse cytomegalovirus glycoprotein retains MHC class I complexes in the ERGIC/cis-Golgi compartments publication-title: Immunity doi: 10.1016/S1074-7613(00)80242-3 contributor: fullname: Ziegler – volume: 190 start-page: 1285 year: 1999 ident: 10.1016/j.immuni.2016.01.010_bib25 article-title: The immunoevasive function encoded by the mouse cytomegalovirus gene m152 protects the virus against T cell control in vivo publication-title: J. Exp. Med. doi: 10.1084/jem.190.9.1285 contributor: fullname: Krmpotic – volume: 12 start-page: 770 year: 2011 ident: 10.1016/j.immuni.2016.01.010_bib37 article-title: Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells publication-title: Nat. Immunol. doi: 10.1038/ni.2050 contributor: fullname: Thiery – volume: 38 start-page: 502 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib22 article-title: Peripheral prepositioning and local CXCL9 chemokine-mediated guidance orchestrate rapid memory CD8+ T cell responses in the lymph node publication-title: Immunity doi: 10.1016/j.immuni.2012.11.012 contributor: fullname: Kastenmüller – volume: 890 start-page: 59 year: 2012 ident: 10.1016/j.immuni.2016.01.010_bib24 article-title: Easy and efficient protocols for working with recombinant vaccinia virus MVA publication-title: Methods Mol. Biol. doi: 10.1007/978-1-61779-876-4_4 contributor: fullname: Kremer – volume: 22 start-page: 402 year: 2010 ident: 10.1016/j.immuni.2016.01.010_bib41 article-title: Designing CD8+ T cell vaccines: it’s not rocket science (yet) publication-title: Curr. Opin. Immunol. doi: 10.1016/j.coi.2010.04.002 contributor: fullname: Yewdell – volume: 274 start-page: 94 year: 1996 ident: 10.1016/j.immuni.2016.01.010_bib1 article-title: Phenotypic analysis of antigen-specific T lymphocytes publication-title: Science doi: 10.1126/science.274.5284.94 contributor: fullname: Altman – volume: 56 start-page: 1458 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib34 article-title: Complex correlates of protection after vaccination publication-title: Clin. Infect. Dis. doi: 10.1093/cid/cit048 contributor: fullname: Plotkin – volume: 9 start-page: 789 year: 2009 ident: 10.1016/j.immuni.2016.01.010_bib3 article-title: Analysing immune cell migration publication-title: Nat. Rev. Immunol. doi: 10.1038/nri2638 contributor: fullname: Beltman – volume: 12 start-page: 879 year: 2011 ident: 10.1016/j.immuni.2016.01.010_bib4 article-title: Afferent lymph-derived T cells and DCs use different chemokine receptor CCR7-dependent routes for entry into the lymph node and intranodal migration publication-title: Nat. Immunol. doi: 10.1038/ni.2085 contributor: fullname: Braun – volume: 31 start-page: 99 year: 2009 ident: 10.1016/j.immuni.2016.01.010_bib26 article-title: Integrin-dependent organization and bidirectional vesicular traffic at cytotoxic immune synapses publication-title: Immunity doi: 10.1016/j.immuni.2009.05.009 contributor: fullname: Liu – volume: 1 start-page: 627 year: 1998 ident: 10.1016/j.immuni.2016.01.010_bib2 article-title: Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT publication-title: Mol. Cell doi: 10.1016/S1097-2765(00)80063-5 contributor: fullname: Aramburu – volume: 35 start-page: 161 year: 2011 ident: 10.1016/j.immuni.2016.01.010_bib42 article-title: CD8(+) T cells: foot soldiers of the immune system publication-title: Immunity doi: 10.1016/j.immuni.2011.07.010 contributor: fullname: Zhang – volume: 13 start-page: 155 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib18 article-title: Anatomically restricted synergistic antiviral activities of innate and adaptive immune cells in the skin publication-title: Cell Host Microbe doi: 10.1016/j.chom.2013.01.004 contributor: fullname: Hickman – volume: 328 start-page: 102 year: 2010 ident: 10.1016/j.immuni.2016.01.010_bib17 article-title: Evasion of CD8+ T cells is critical for superinfection by cytomegalovirus publication-title: Science doi: 10.1126/science.1185350 contributor: fullname: Hansen – volume: 92 start-page: 1279 year: 2011 ident: 10.1016/j.immuni.2016.01.010_bib30 article-title: Single cell detection of latent cytomegalovirus reactivation in host tissue publication-title: J. Gen. Virol. doi: 10.1099/vir.0.029827-0 contributor: fullname: Marquardt – volume: 221 start-page: 77 year: 2008 ident: 10.1016/j.immuni.2016.01.010_bib9 article-title: T-cell activation through immunological synapses and kinapses publication-title: Immunol. Rev. doi: 10.1111/j.1600-065X.2008.00589.x contributor: fullname: Dustin – volume: 499 start-page: 295 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib6 article-title: Ultrasensitive fluorescent proteins for imaging neuronal activity publication-title: Nature doi: 10.1038/nature12354 contributor: fullname: Chen – volume: 11 start-page: e1004178 year: 2015 ident: 10.1016/j.immuni.2016.01.010_bib13 article-title: Estimating the In Vivo Killing Efficacy of Cytotoxic T Lymphocytes across Different Peptide-MHC Complex Densities publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1004178 contributor: fullname: Garcia – volume: 38 start-page: 237 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib28 article-title: The transcription factor NFAT exhibits signal memory during serial T cell interactions with antigen-presenting cells publication-title: Immunity doi: 10.1016/j.immuni.2012.09.012 contributor: fullname: Marangoni – volume: 36 start-page: 142 year: 2012 ident: 10.1016/j.immuni.2016.01.010_bib33 article-title: Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes publication-title: Immunity doi: 10.1016/j.immuni.2012.01.002 contributor: fullname: Newell – volume: 251 start-page: 154 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib8 article-title: Dynamics of NK cell interactions in vivo publication-title: Immunol. Rev. doi: 10.1111/imr.12015 contributor: fullname: Deguine – volume: 110 start-page: 9090 year: 2013 ident: 10.1016/j.immuni.2016.01.010_bib7 article-title: In vivo imaging of CD8+ T cell-mediated elimination of malaria liver stages publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1303858110 contributor: fullname: Cockburn – volume: 42 start-page: 524 year: 2015 ident: 10.1016/j.immuni.2016.01.010_bib19 article-title: CXCR3 chemokine receptor enables local CD8(+) T cell migration for the destruction of virus-infected cells publication-title: Immunity doi: 10.1016/j.immuni.2015.02.009 contributor: fullname: Hickman – volume: 35 start-page: 285 year: 2011 ident: 10.1016/j.immuni.2016.01.010_bib39 article-title: Tight regulation of memory CD8(+) T cells limits their effectiveness during sustained high viral load publication-title: Immunity doi: 10.1016/j.immuni.2011.05.017 contributor: fullname: West – volume: 42 start-page: 864 year: 2015 ident: 10.1016/j.immuni.2016.01.010_bib36 article-title: Actin depletion initiates events leading to granule secretion at the immunological synapse publication-title: Immunity doi: 10.1016/j.immuni.2015.04.013 contributor: fullname: Ritter – volume: 427 start-page: 154 year: 2004 ident: 10.1016/j.immuni.2016.01.010_bib31 article-title: T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases publication-title: Nature doi: 10.1038/nature02238 contributor: fullname: Mempel – volume: 172 start-page: 6944 year: 2004 ident: 10.1016/j.immuni.2016.01.010_bib15 article-title: Murine cytomegalovirus interference with antigen presentation has little effect on the size or the effector memory phenotype of the CD8 T cell response publication-title: J. Immunol. doi: 10.4049/jimmunol.172.11.6944 contributor: fullname: Gold – volume: 23 start-page: 249 year: 2005 ident: 10.1016/j.immuni.2016.01.010_bib23 article-title: Perforin triggers a plasma membrane-repair response that facilitates CTL induction of apoptosis publication-title: Immunity doi: 10.1016/j.immuni.2005.08.001 contributor: fullname: Keefe – volume: 8 start-page: e1002381 year: 2012 ident: 10.1016/j.immuni.2016.01.010_bib10 article-title: The efficiency of the human CD8+ T cell response: how should we quantify it, what determines it, and does it matter? publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1002381 contributor: fullname: Elemans – volume: 10 start-page: e1003534 year: 2014 ident: 10.1016/j.immuni.2016.01.010_bib11 article-title: Rates of CTL killing in persistent viral infection in vivo publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1003534 contributor: fullname: Elemans – volume: 206 start-page: 2593 year: 2009 ident: 10.1016/j.immuni.2016.01.010_bib16 article-title: Induced bronchus-associated lymphoid tissue serves as a general priming site for T cells and is maintained by dendritic cells publication-title: J. Exp. Med. doi: 10.1084/jem.20091472 contributor: fullname: Halle – volume: 10 start-page: e1003805 year: 2014 ident: 10.1016/j.immuni.2016.01.010_bib20 article-title: Spatial heterogeneity and peptide availability determine CTL killing efficiency in vivo publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1003805 contributor: fullname: Hogan |
SSID | ssj0014590 |
Score | 2.5932937 |
Snippet | According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated... According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated... |
SourceID | pubmedcentral proquest crossref pubmed elsevier |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 233 |
SubjectTerms | Animals Antigens Apoptosis Calcium Signaling Cell Communication Cells, Cultured Confidence intervals Cytokines Cytotoxicity Cytotoxicity, Immunologic Experiments Fibroblasts Herpesviridae Infections - immunology Humans Immune Evasion Infections Lymphatic system Lymphocytes Mathematical models Mice Mice, Inbred C57BL Mice, Knockout Microscopy Microscopy, Fluorescence, Multiphoton Muromegalovirus - immunology Perforin - genetics Perforin - metabolism Rodents Statistical analysis T cell receptors T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - virology T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - virology Vaccinia - immunology Vaccinia virus - immunology Viral infections Viruses |
Title | In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity |
URI | https://dx.doi.org/10.1016/j.immuni.2016.01.010 https://www.ncbi.nlm.nih.gov/pubmed/26872694 https://www.proquest.com/docview/1766575615/abstract/ https://search.proquest.com/docview/1767068609 https://search.proquest.com/docview/1768579232 https://pubmed.ncbi.nlm.nih.gov/PMC4846978 |
Volume | 44 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3bbtQwELWqIhAviJbb0ouMxGu0uXvz2AaqLkt5gLbsm2U7tgiqklWTVvRv-i18GTO2E3ULAglppdWuJ1ES2zPHmTnHhLw1sERgMxkHELpNkAJkDgS4wCARESqQSZh_-Grg5FN-fJZ-WGbLDVIOXBgsq_S-3_l06639P1P_NKerup5-wVJCWIQnUW7zYeiHUdsTSXzLwzGTkGZFONYdgvVAn7M1XrXlYGCBV27FO5FH--fw9Dv8vF9FeScsHT0lTzyepAfukrfIhm62yUO3w-TNNnl04nPnz0g_b37entfXLV3UVoiblhAoFaBw2hpa3vRt3_6oFT2lpb646Oi8o579REVT0XkDjuxad_Sd28Oe2neJjhbRWQt3IC3bdqUv_a4Uz8nZ0fvT8jjwey4EKovTPkCmqTLMwMJYMJVGKi6UMUJhwjSFlhwCnkwUA9ghs6QqpDJFWMSCWSVCwZIXZLNpG_2K0Co2lQBIII0WKZxSGkAXUhdSVkUsZTYhwfCo-cpJa_Ch5uw7d13DsWt4GMEnnBA29AdfGyIcvP8_jtwduo_7KdpxVMYErAqIbkLejM0wuTBjIhrdXlkbhhyasPirzSxDFcZ4Ql66ETHeTpzPGFKF4dLXxspogOLe6y1N_c2KfKcADGGF__q_b3qHPMZfWGEe5btks7-80nsAoHq5Tx4cHJ4vPuL34vPXxb6dMb8Aq58eEA |
link.rule.ids | 230,315,786,790,891,3525,27602,27957,27958,45698,45909 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3bbtQwELWqIi4vqJTbQgtG4jXazdWbRwitNrTbF7Zo3yzbsUVQlayatKJ_w7fwZczYTsQWBBLSPsWTVeLLzHFmzjEhbw1sEdhcRgGEbhMkAJkDAS4wiEWICmQS1h9-GlieZYvz5OM6Xe-QYuDCYFml9_3Op1tv7a9MfW9ON3U9_YSlhLAJj8PM5sPAD99J0nyGU7tcvx9TCXhtLDwE84E_Z4u8akvCwAqvzKp3IpH2z_Hpd_x5u4zyl7h0vEceekBJ37lnfkR2dLNP7rojJm_2yb2lT54_Jn3Z_Pj-ub5u6UltlbhpAZFSAQynraHFTd_27bda0RUt9MVFR8uOevoTFU1FywY82bXu6Ad3iD21HxMdL6KzFu5GWrTtRl_6YymekPPjo1WxCPyhC4FKo6QPkGqqDDOwMxZMJaGKcmWMUJgxTaAlg4gnY8UAd8g0rnKpDPR5JJiVIhQsfkp2m7bRzwmtIlMJwATSaJHAX0oD8ELqXMoqj6RMJyQYuppvnLYGH4rOvnI3NByHhs9C-M0mhA3jwbfmCAf3_487D4bh436NdhylMQGsAqSbkDdjM6wuTJmIRrdX1oYhiWaW_9VmnqIMYzQhz9yMGF8nyuYMucLw6FtzZTRAde_tlqb-YlW-E0CGsMV_8d8v_ZrcX6yWp_y0PDt5SR5gC5abh9kB2e0vr_QhoKlevrKr5Scs5B4B |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In%C2%A0Vivo+Killing+Capacity+of+Cytotoxic+T+Cells+Is+Limited+and+Involves+Dynamic+Interactions+and+T+Cell+Cooperativity&rft.jtitle=Immunity+%28Cambridge%2C+Mass.%29&rft.au=Halle%2C+Stephan&rft.au=Keyser%2C+Kirsten+Anja&rft.au=Stahl%2C+Felix+Rolf&rft.au=Busche%2C+Andreas&rft.date=2016-02-16&rft.issn=1074-7613&rft.volume=44&rft.issue=2&rft.spage=233&rft.epage=245&rft_id=info:doi/10.1016%2Fj.immuni.2016.01.010&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_immuni_2016_01_010 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1074-7613&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1074-7613&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1074-7613&client=summon |