Bioluminescence resonance energy transfer (BRET) imaging of protein–protein interactions within deep tissues of living subjects

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 29; pp. 12060 - 12065
• Main Authors: Dragulescu-Andrasi, Anca, Chan, Carmel T., De, Abhijit, Massoud, Tarik F., Gambhir, Sanjiv S.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 19.07.2011; National Acad Sciences
• Subjects: animal disease models; Animal diseases; Animals; binding proteins; Biological Sciences; Bioluminescence; Blotting, Western; Cell culture; Cell culture techniques; Drug Evaluation, Preclinical - methods; Drug interactions; drugs; Energy transfer; Fluorescence Resonance Energy Transfer - methods; fluorescent proteins; image analysis; Imaging; Imidazoles; luciferase; Luciferases, Renilla; Luminescent Proteins; Mice; model validation; Plasmids - genetics; Protein Binding; protein-protein interactions; Proteins; Pyrazines; Ratios; Red Fluorescent Protein; Resonance; screening; Sensors; Tacrolimus Binding Protein 1A - metabolism; Tissues; TOR Serine-Threonine Kinases - metabolism; Transcriptional regulatory elements; Wavelengths

Identifying protein–protein interactions (PPIs) is essential for understanding various disease mechanisms and developing new therapeutic approaches. Current methods for assaying cellular intermolecular interactions are mainly used for cells in culture and have limited use for the noninvasive assessm...