Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing li...
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| Published in | Genome research Vol. 24; no. 12; pp. 2033 - 2040 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Cold Spring Harbor Laboratory Press
01.12.2014
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. |
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| AbstractList | Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications.Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from subpicogram amounts of cDNA. The comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, because naked Tn5 can be annealed to any oligonucleotide of choice, for example, molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enable innovation in sequencing-based applications. |
| Author | Reinius, Björn Sagasser, Sven Picelli, Simone Björklund, Åsa K. Winberg, Gösta Sandberg, Rickard |
| Author_xml | – sequence: 1 givenname: Simone surname: Picelli fullname: Picelli, Simone – sequence: 2 givenname: Åsa K. surname: Björklund fullname: Björklund, Åsa K. – sequence: 3 givenname: Björn surname: Reinius fullname: Reinius, Björn – sequence: 4 givenname: Sven surname: Sagasser fullname: Sagasser, Sven – sequence: 5 givenname: Gösta surname: Winberg fullname: Winberg, Gösta – sequence: 6 givenname: Rickard surname: Sandberg fullname: Sandberg, Rickard |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25079858$$D View this record in MEDLINE/PubMed http://kipublications.ki.se/Default.aspx?queryparsed=id:130237328$$DView record from Swedish Publication Index |
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| Cites_doi | 10.1073/pnas.80.19.5852 10.1074/jbc.273.13.7367 10.1101/gr.136242.111 10.1186/gb-2010-11-12-r119 10.1046/j.1365-2958.2003.03382.x 10.1101/SQB.1979.043.01.046 10.1038/nmeth.2639 10.1038/nprot.2013.118 10.1146/annurev.genet.42.110807.091656 10.1074/jbc.274.52.37021 10.1038/nprot.2014.006 10.1038/nmeth.2769 10.1038/nsb778 10.1038/nmeth.2772 10.1038/nmeth.2764 10.1126/science.1247651 10.1186/1471-2180-11-38 10.1016/j.celrep.2012.08.003 10.1038/nrg2626 |
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