Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing

• Published in: Journal of thrombosis and haemostasis Vol. 12; no. 7; pp. 1182 - 1192
• Main Authors: Pol, E., Coumans, F. A. W., Grootemaat, A. E., Gardiner, C., Sargent, I. L., Harrison, P., Sturk, A., Leeuwen, T. G., Nieuwland, R.
• Format: Journal Article
• Language: English
• Published: England Elsevier Limited 01.07.2014
• Subjects: Biomarkers - metabolism; Cell-Derived Microparticles - metabolism; cell‐derived microparticles; exosomes; Exosomes - metabolism; Flow Cytometry; Humans; Microscopy, Electron, Transmission; Nanoparticles - chemistry; optical devices; Optics and Photonics; Particle Size; reference standards; Refractometry; Reproducibility of Results; secretory vesicles; cell-derived microparticles; optical devices; reference standards; exosomes; secretory vesicles

Summary Background Enumeration of extracellular vesicles has clinical potential as a biomarker for disease. In biological samples, the smallest and largest vesicles typically differ 25‐fold in size, 300 000‐fold in concentration, 20 000‐fold in volume, and 10 000 000‐fold in scattered light. Because...