Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes

• Published in: Indian journal of pharmacology Vol. 49; no. 2; pp. 155 - 160
• Main Authors: Shamsi, ToobaNaz, Parveen, Romana, Fatima, Sadaf
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.03.2017; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: A549 Cells; Acids; Antineoplastic Agents - pharmacology; Antioxidants; Antioxidants (Nutrients); Antioxidants - pharmacology; Apoptosis; Biotechnology; Cancer; Cell adhesion & migration; Cell cycle; Cell growth; Cell Proliferation - drug effects; Cell Survival - drug effects; Cytotoxicity; Enzymes; Garlic; Glutathione Peroxidase - metabolism; Glutathione Transferase - metabolism; HEK293 Cells; Humans; Metastasis; Oxidative stress; Phaseolus; Physiological aspects; Reactive oxygen species; Reactive Oxygen Species - metabolism; Seeds; Signal transduction; Superoxide Dismutase - metabolism; Trypsin inhibitors; Trypsin Inhibitors - pharmacology; Vigna; lung cancer; Antioxidant; protein; protein protease inhibitors
• DOI: 10.4103/ijp.IJP_553_16
• ISSN: 0253-7613; 1998-3751

Protease inhibitors are one of the most promising and investigated subjects for their role in pharmacognostical and pharmacological studies. This study aimed to investigate antineoplastic and antioxidant activity of trypsin inhibitors (TIs) isolated from three plant sources and their inhibitory role in the cell line. TIs were obtained from different plant sources. Antineoplastic potential on adenocarcinoma human alveolar basal epithelial cell line (A549) and normal Human Embryonic Kidney (HEK) was determined using MTT assay. Activities of antioxidant enzyme, nitric oxide scavenger, superoxide dismutase, glutathione S-transferase, and glutathione peroxidase were assessed in cell lines incubated with and without TIs. The outcome was analyzed by spectrophotometer. TIs showed the higher cytotoxicity on A549 cells as compared to normal HEK cell line. TIs exhibited fair increase in antioxidant enzyme activity in A549 cells as compared to control. This might be one of the strategies of antineoplastic effect in cancer cells. This study has reported the antioxidant and antineoplastic properties of these TIs for the first time in A549 cells (to the best of our knowledge). The results show that TIs possess ability to prevent cancer and diseases caused due to oxidative stress. Therefore, we conclude that TIs can be used as supplements along with the conventional drugs for increased efficacy in the treatment of diseases such as cardiovascular disease, atherosclerosis, and cancer.