Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil

A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly,...

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Published inThe journal of microbiology Vol. 49; no. 1; pp. 29 - 34
Main Authors Kang, Y.S., Korea University, Seoul, Republic of Korea, Jung, J.J., Korea University, Seoul, Republic of Korea, Jeon, C.O., Chung-Ang University, Seoul, Republic of Korea, Park, W.J., Korea University, Seoul, Republic of Korea
Format Journal Article
LanguageEnglish
Published Heidelberg The Microbiological Society of Korea 01.02.2011
Springer Nature B.V
한국미생물학회
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Online AccessGet full text
ISSN1225-8873
1976-3794
1976-3794
DOI10.1007/s12275-011-0315-y

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Abstract A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly, strain DR1∨T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 4:1.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1∨T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1∨T in the genus Acinetobacter as a,novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1∨T (=KCTC 23045∨T =JCM 16667∨T).
AbstractList A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).
A diesel-oil and n -hexadecane-degrading novel bacterial strain, designated DR1 T , was isolated from a rice paddy in Deok-So, South Korea. The strain DR1 T cells were Gram-negative, aerobic coccobacilli, and grew at 20–37°C with the optimal temperature of 30°C, and an optimal pH of 6–8. Interestingly, strain DR1 T was highly motile (swimming and swarming motility) using its fimbriae, and generated N -acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi , and A. beijerinckii , with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1 T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1 T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1 T (=KCTC 23045 T =JCM 16667 T ).
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1T cells were Gram-negative, aerobic coccobacilli, and grew at 20--37ADGC with the optimal temperature of 30ADGC, and an optimal pH of 6--8. Interestingly, strain DR1T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1T (=KCTC 23045T =JCM 16667T).
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly, strain DR1∨T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 4:1.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1∨T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1∨T in the genus Acinetobacter as a,novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1∨T (=KCTC 23045∨T =JCM 16667∨T).
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1^T cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1^T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9)and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%,respectively. DNA-DNA hybridization values between strain DR1^T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1^T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1^T (=KCTC 23045^T =JCM 16667^T). KCI Citation Count: 53
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^sup T^, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1^sup T^ cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1^sup T^ was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1^sup T^ and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1^sup T^ in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1^sup T^ (=KCTC 23045^sup T^ =JCM 16667^sup T^).[PUBLICATION ABSTRACT]
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).
Author Kang, Y.S., Korea University, Seoul, Republic of Korea
Jeon, C.O., Chung-Ang University, Seoul, Republic of Korea
Park, W.J., Korea University, Seoul, Republic of Korea
Jung, J.J., Korea University, Seoul, Republic of Korea
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  fullname: Park, W.J., Korea University, Seoul, Republic of Korea
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Keywords genome
diesel
biodegradation
soil
bacteria
biofilm
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17911292 - Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2259-61
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5963505 - J Gen Microbiol. 1966 May;43(2):159-271
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16520925 - Appl Microbiol Biotechnol. 2006 Sep;72(2):353-60
19661501 - Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):896-903
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Snippet A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T...
A diesel-oil and n -hexadecane-degrading novel bacterial strain, designated DR1 T , was isolated from a rice paddy in Deok-So, South Korea. The strain DR1 T...
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T)...
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^sup T^, was isolated from a rice paddy in Deok-So, South Korea. The strain...
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1T cells...
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1^T...
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StartPage 29
SubjectTerms Acinetobacter
Acinetobacter - growth & development
Acinetobacter - metabolism
Acinetobacter - physiology
Alkanes - metabolism
BACTERIA
Bacterial Adhesion
Bacterial Typing Techniques
Base Composition
BIODEGRADACION
BIODEGRADATION
biofilm
Biomedical and Life Sciences
Cluster Analysis
Data processing
Deoxyribonucleic acid
diesel
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Environmental Microbiology
Gasoline - microbiology
GENOMAS
GENOME
GENOMES
Hybridization
Hydrocarbons
Hydrogen-Ion Concentration
Korea
Life Sciences
Locomotion
Microbiology
Molecular Sequence Data
Motility
N-Acyl homoserine lactone
New species
Nucleic Acid Hybridization
Oryza - growth & development
Oryza sativa
pH effects
Phylogenetics
Phylogeny
Pili
Quinone
Quinones - analysis
quorum sensing
Rice fields
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
SOIL
SOL
SUELO
Swarming
Swimming
Temperature
Temperature effects
생물학
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Title Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil
URI https://link.springer.com/article/10.1007/s12275-011-0315-y
https://www.ncbi.nlm.nih.gov/pubmed/21369976
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Volume 49
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ispartofPNX The Journal of Microbiology, 2011, 49(1), , pp.29-34
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