Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly,...
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Published in | The journal of microbiology Vol. 49; no. 1; pp. 29 - 34 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
The Microbiological Society of Korea
01.02.2011
Springer Nature B.V 한국미생물학회 |
Subjects | |
Online Access | Get full text |
ISSN | 1225-8873 1976-3794 1976-3794 |
DOI | 10.1007/s12275-011-0315-y |
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Abstract | A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly, strain DR1∨T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 4:1.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1∨T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1∨T in the genus Acinetobacter as a,novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1∨T (=KCTC 23045∨T =JCM 16667∨T). |
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AbstractList | A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)). A diesel-oil and n -hexadecane-degrading novel bacterial strain, designated DR1 T , was isolated from a rice paddy in Deok-So, South Korea. The strain DR1 T cells were Gram-negative, aerobic coccobacilli, and grew at 20–37°C with the optimal temperature of 30°C, and an optimal pH of 6–8. Interestingly, strain DR1 T was highly motile (swimming and swarming motility) using its fimbriae, and generated N -acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi , and A. beijerinckii , with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1 T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1 T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1 T (=KCTC 23045 T =JCM 16667 T ). A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1T cells were Gram-negative, aerobic coccobacilli, and grew at 20--37ADGC with the optimal temperature of 30ADGC, and an optimal pH of 6--8. Interestingly, strain DR1T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1T (=KCTC 23045T =JCM 16667T). A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly, strain DR1∨T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 4:1.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1∨T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1∨T in the genus Acinetobacter as a,novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1∨T (=KCTC 23045∨T =JCM 16667∨T). A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1^T cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1^T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9)and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%,respectively. DNA-DNA hybridization values between strain DR1^T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1^T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1^T (=KCTC 23045^T =JCM 16667^T). KCI Citation Count: 53 A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^sup T^, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1^sup T^ cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1^sup T^ was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1^sup T^ and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1^sup T^ in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1^sup T^ (=KCTC 23045^sup T^ =JCM 16667^sup T^).[PUBLICATION ABSTRACT] A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)). |
Author | Kang, Y.S., Korea University, Seoul, Republic of Korea Jeon, C.O., Chung-Ang University, Seoul, Republic of Korea Park, W.J., Korea University, Seoul, Republic of Korea Jung, J.J., Korea University, Seoul, Republic of Korea |
Author_xml | – sequence: 1 fullname: Kang, Y.S., Korea University, Seoul, Republic of Korea – sequence: 2 fullname: Jung, J.J., Korea University, Seoul, Republic of Korea – sequence: 3 fullname: Jeon, C.O., Chung-Ang University, Seoul, Republic of Korea – sequence: 4 fullname: Park, W.J., Korea University, Seoul, Republic of Korea |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21369976$$D View this record in MEDLINE/PubMed https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001532725$$DAccess content in National Research Foundation of Korea (NRF) |
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Keywords | genome diesel biodegradation soil bacteria biofilm |
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Snippet | A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T... A diesel-oil and n -hexadecane-degrading novel bacterial strain, designated DR1 T , was isolated from a rice paddy in Deok-So, South Korea. The strain DR1 T... A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T)... A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^sup T^, was isolated from a rice paddy in Deok-So, South Korea. The strain... A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1T cells... A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1^T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1^T... |
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SubjectTerms | Acinetobacter Acinetobacter - growth & development Acinetobacter - metabolism Acinetobacter - physiology Alkanes - metabolism BACTERIA Bacterial Adhesion Bacterial Typing Techniques Base Composition BIODEGRADACION BIODEGRADATION biofilm Biomedical and Life Sciences Cluster Analysis Data processing Deoxyribonucleic acid diesel DNA DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Environmental Microbiology Gasoline - microbiology GENOMAS GENOME GENOMES Hybridization Hydrocarbons Hydrogen-Ion Concentration Korea Life Sciences Locomotion Microbiology Molecular Sequence Data Motility N-Acyl homoserine lactone New species Nucleic Acid Hybridization Oryza - growth & development Oryza sativa pH effects Phylogenetics Phylogeny Pili Quinone Quinones - analysis quorum sensing Rice fields RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA SOIL SOL SUELO Swarming Swimming Temperature Temperature effects 생물학 |
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Title | Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil |
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