Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil

• Published in: The journal of microbiology Vol. 49; no. 1; pp. 29 - 34
• Main Authors: Kang, Y.S., Korea University, Seoul, Republic of Korea, Jung, J.J., Korea University, Seoul, Republic of Korea, Jeon, C.O., Chung-Ang University, Seoul, Republic of Korea, Park, W.J., Korea University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.02.2011; Springer Nature B.V; 한국미생물학회
• Subjects: Acinetobacter; Acinetobacter - growth & development; Acinetobacter - metabolism; Acinetobacter - physiology; Alkanes - metabolism; BACTERIA; Bacterial Adhesion; Bacterial Typing Techniques; Base Composition; BIODEGRADACION; BIODEGRADATION; biofilm; Biomedical and Life Sciences; Cluster Analysis; Data processing; Deoxyribonucleic acid; diesel; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Environmental Microbiology; Gasoline - microbiology; GENOMAS; GENOME; GENOMES; Hybridization; Hydrocarbons; Hydrogen-Ion Concentration; Korea; Life Sciences; Locomotion; Microbiology; Molecular Sequence Data; Motility; N-Acyl homoserine lactone; New species; Nucleic Acid Hybridization; Oryza - growth & development; Oryza sativa; pH effects; Phylogenetics; Phylogeny; Pili; Quinone; Quinones - analysis; quorum sensing; Rice fields; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; SOIL; SOL; SUELO; Swarming; Swimming; Temperature; Temperature effects; 생물학; Korea; South Korea; genome; diesel; biodegradation; soil; bacteria; biofilm
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-011-0315-y

A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1∨T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1∨T cells were Grain-negative, aerobic coccobacilli, and grew at 20-37℃ with the optimal temperature of 30℃, and an optimal pH of 6-8. Interestingly, strain DR1∨T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 4:1.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1∨T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1∨T in the genus Acinetobacter as a,novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1∨T (=KCTC 23045∨T =JCM 16667∨T).