TEF-7A, a transcript elongation factor gene, influences yield-related traits in bread wheat (Triticum aestivum L.)

• Published in: Journal of experimental botany Vol. 65; no. 18; pp. 5351 - 5365
• Main Authors: Zheng, Jun, Liu, Hong, Wang, Yuquan, Wang, Lanfen, Chang, Xiaoping, Jing, Ruilian, Hao, Chenyang, Zhang, Xueyong
• Format: Journal Article
• Language: English
• Published: England Oxford University Press [etc.] 01.10.2014; Oxford University Press
• Subjects: Chromosomes; Gene expression; Genes; Genomes; Geographic regions; Grains; Haplotypes; Haplotypes - genetics; marker-assisted selection; Peptide Elongation Factors - metabolism; Phenotypic traits; Plant Proteins - metabolism; Polymerase chain reaction; Promoter regions; RESEARCH PAPER; Triticum - metabolism; Triticum aestivum; wheat; Association analysis; haplotypes; wheat; transcript elongation factor; yield
• DOI: 10.1093/jxb/eru306
• ISSN: 0022-0957; 1460-2431

In this study, TaTEF-7A, a member of the transcript elongation factor gene family, and its flanking sequences were isolated. TaTEF-7A was located on chromosome 7A and was flanked by markers Xwmc83 and XP3156.3. Subcellular localization revealed that TaTEF-7A protein was localized in the nucleus. This gene was expressed in all organs, but the highest expression occurred in young spikes and developing seeds. Overexpression of TaTEF-7A in Arabidopsis thaliana produced pleiotropic effects on vegetative and reproductive development that enhanced grain length, silique number, and silique length. No diversity was found in the coding region of TaTEF-7A, but 16 single nucleotide polymorphisms and Indels were detected in the promoter regions of different cultivars. Markers based on sequence variations in the promoter regions (InDel-629 and InDel-604) were developed, and three haplotypes were identified based on those markers. Haplotype-trait association analysis of the Chinese wheat mini core collection revealed that TaTEF-7A was significantly associated with grain number per spike. Phenotyping of near-isogenic lines (NILs) confirmed that TaTEF-7A increases potential grain yield and yield-related traits. Frequency changes in favoured haplotypes gradually increased in cultivars released in China from the 1940s. Geographic distributions of favoured haplotypes were characterized in six major wheat production regions worldwide. The presence of Hap-7A-3, the favoured haplotype, showed a positive correlation with yield in a global set of breeding lines. These results suggest that TaTEF-7A is a functional regulatory factor for grain number per spike and provide a basis for marker-assisted selection.