Fork-Like DNA Templates Support Bypass Replication of Lesions that Block DNA Synthesis on Single-Stranded Templates
DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on...
Saved in:
| Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 24; pp. 13766 - 13769 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
National Academy of Sciences of the United States of America
26.11.1996
National Acad Sciences National Academy of Sciences The National Academy of Sciences of the USA |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N-2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA. |
|---|---|
| AbstractList | DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N-2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA. DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N -2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA. Hoffmann et al examined the effect of the asymmetry of DNA replication on the efficiency of translesion synthesis. Results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA. DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N -2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA. double- and single-stranded DNA replication cell extracts translesion synthesis cisplatin and N-2-acetylaminofluorene adducts |
| Author | Robert P. P. Fuchs Defais, Martine Burnouf, Dominique Villani, Giuseppe Lesca, Claire Hoffmann, Jean-Sebastien Pillaire, Marie-Jeanne |
| AuthorAffiliation | Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9062, 205, route de Narbonne, 31 077 Toulouse, Cedex, France; and † Unité Propre de Recherche 9003 de Cancérogenèse et Mutagenèse Moléculaire et Structurale, Centre National de la Recherche Scientifique, Institut de Recherche sur le Cancer de L’Appareil Digestif, B.P. 426, 67091 Strasbourg Cedex, France |
| AuthorAffiliation_xml | – name: Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9062, 205, route de Narbonne, 31 077 Toulouse, Cedex, France; and † Unité Propre de Recherche 9003 de Cancérogenèse et Mutagenèse Moléculaire et Structurale, Centre National de la Recherche Scientifique, Institut de Recherche sur le Cancer de L’Appareil Digestif, B.P. 426, 67091 Strasbourg Cedex, France |
| Author_xml | – sequence: 1 givenname: Jean-Sebastien surname: Hoffmann fullname: Hoffmann, Jean-Sebastien – sequence: 2 givenname: Marie-Jeanne surname: Pillaire fullname: Pillaire, Marie-Jeanne – sequence: 3 givenname: Claire surname: Lesca fullname: Lesca, Claire – sequence: 4 givenname: Dominique surname: Burnouf fullname: Burnouf, Dominique – sequence: 5 fullname: Robert P. P. Fuchs – sequence: 6 givenname: Martine surname: Defais fullname: Defais, Martine – sequence: 7 givenname: Giuseppe surname: Villani fullname: Villani, Giuseppe |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8943009$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kUFvEzEQhS1UVNLCHSEhVhwqLhvstTe2JS6lpRQpAomUs-XsjptNHHtrexH59zhNSCkHTh7rfW_0Ru8EHTnvAKGXBI8J5vR973QcSzqu2JhQPpk8QSOCJSknTOIjNMK44qVgFXuGTmJcYoxlLfAxOhaS0fwZoXjlw6qcdisoLr-eFzew7q1OEIvZ0Pc-pOLjptcxFt-ht12jU-dd4U0xhZinWKSFzoj1zerePtu4tMhSLDI269ythXKWgnYttA-7n6OnRtsIL_bvKfpx9enm4rqcfvv85eJ8WjZ1RVMJmGCdr2lrAbQ1kkpjsJZNy4wUdVPPqWk5mXDZaGq4yEKF-ZwIQQWwOW3pKfqw29sP8zW0Dbgcxao-dGsdNsrrTj1WXLdQt_6nIpIRme1ne3vwdwPEpNZdbMBa7cAPUZGacyF4lcG3_4BLPwSXT1MVJpSQqt5CeAc1wccYwBxyEKy2Xaptl0pSVTF132W2vP47_8GwLy_rb_b61vlHfbzh3f8JZQZrE_xKGX21Q5cx-XBgGZa8pr8BuaC_XQ |
| CitedBy_id | crossref_primary_10_1016_S0027_5107_01_00141_5 crossref_primary_10_1073_pnas_95_21_12586 crossref_primary_10_1093_toxsci_kfy007 crossref_primary_10_1007_s00428_013_1516_z crossref_primary_10_1002__SICI_1098_2280_1999_34_2_3_72__AID_EM3_3_0_CO_2_8 crossref_primary_10_1016_j_semcancer_2010_10_001 crossref_primary_10_1038_sj_onc_1202619 crossref_primary_10_1124_mol_56_2_390 crossref_primary_10_1016_S0921_8777_97_00058_X crossref_primary_10_1016_S0300_9084_00_00340_0 crossref_primary_10_1128_MCB_19_3_2206 crossref_primary_10_1096_fj_99_1063com crossref_primary_10_1128_MCB_18_2_960 crossref_primary_10_1016_S0162_0134_99_00149_X crossref_primary_10_1016_j_dnarep_2005_06_010 crossref_primary_10_1006_jmbi_1997_1143 crossref_primary_10_1038_sj_onc_1204743 crossref_primary_10_1074_jbc_272_21_13945 crossref_primary_10_1016_S0162_0134_99_00134_8 crossref_primary_10_1054_drup_1999_0099 crossref_primary_10_1158_0008_5472_CAN_03_3942 crossref_primary_10_1128_MCB_19_1_147 crossref_primary_10_1074_jbc_273_22_13801 crossref_primary_10_1158_1078_0432_CCR_05_1380 crossref_primary_10_1182_blood_2002_10_3207 crossref_primary_10_1016_S1074_5521_96_90175_1 crossref_primary_10_1038_sj_onc_1205295 crossref_primary_10_1074_jbc_M207101200 |
| Cites_doi | 10.1073/pnas.92.12.5356 10.1021/bi00660a027 10.1006/jmbi.1994.1125 10.1074/jbc.271.26.15386 10.1093/nar/20.24.6473 10.1016/S0021-9258(18)82201-3 10.1016/S0092-8674(00)81039-9 10.1016/S0021-9258(17)42371-4 10.1073/pnas.91.16.7752 10.1126/science.8342022 10.1038/377649a0 10.1021/bi00144a021 10.1021/bi00169a018 10.1073/pnas.87.16.6087 10.1016/0022-2836(94)90010-8 10.1074/jbc.270.36.21226 10.1038/369574a0 10.1038/294657a0 10.1128/mcb.13.1.533-542.1993 10.1016/S0021-9258(17)31859-8 10.1093/nar/22.13.2519 10.1016/0076-6879(95)62037-0 10.1002/j.1460-2075.1994.tb06826.x 10.1128/MCB.16.5.2537 10.1016/0921-8777(93)90062-L 10.1038/352544a0 10.1073/pnas.92.26.11975 10.1021/bi00764a017 |
| ContentType | Journal Article |
| Copyright | Copyright 1996 National Academy of Sciences Copyright National Academy of Sciences Nov 26, 1996 Copyright © 1996, The National Academy of Sciences of the USA 1996 |
| Copyright_xml | – notice: Copyright 1996 National Academy of Sciences – notice: Copyright National Academy of Sciences Nov 26, 1996 – notice: Copyright © 1996, The National Academy of Sciences of the USA 1996 |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 5PM |
| DOI | 10.1073/pnas.93.24.13766 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Nucleic Acids Abstracts Ecology Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Entomology Abstracts Genetics Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Immunology Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts |
| DatabaseTitleList | MEDLINE CrossRef Virology and AIDS Abstracts Nucleic Acids Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) |
| EISSN | 1091-6490 |
| EndPage | 13769 |
| ExternalDocumentID | 10708996 10_1073_pnas_93_24_13766 8943009 93_24_13766 40975 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Feature |
| GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 123 29P 2AX 2FS 2WC 3O- 4.4 53G 5RE 5VS 79B 85S AACGO AAFWJ AANCE ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACIWK ACKIV ACNCT ACPRK ADULT ADZLD AENEX AEUPB AEXZC AFDAS AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM ASUFR AS~ CS3 D0L DCCCD DIK DNJUQ DOOOF DU5 DWIUU E3Z EBS EJD F20 F5P FRP GX1 HGD HH5 HQ3 HTVGU HYE JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST KQ8 L7B LU7 MVM N9A NEJ NHB N~3 O9- OK1 P-O PNE PQQKQ R.V RHF RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR VOH VQA W8F WH7 WHG WOQ WOW X7M XFK XSW Y6R YKV YSK ZA5 ZCA ZCG ~02 ~KM - 02 08R 0R 1AW 55 AAPBV ABFLS ABPTK ADACO AJYGW AS DZ GJ KM OHM PQEST X XHC ADACV CGR CUY CVF ECM EIF H13 IPSME NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 5PM |
| ID | FETCH-LOGICAL-c523t-e010a109d58e3df939ff0a9cd4f985c5b3fd71679ca3f789cd207b18838e4b3d3 |
| IEDL.DBID | RPM |
| ISSN | 0027-8424 |
| IngestDate | Tue Sep 17 21:13:49 EDT 2024 Sat Oct 26 00:00:47 EDT 2024 Thu Oct 10 16:43:28 EDT 2024 Fri Aug 23 02:52:40 EDT 2024 Sat Sep 28 08:34:41 EDT 2024 Wed Nov 11 00:29:45 EST 2020 Wed May 29 08:07:27 EDT 2019 Fri Feb 02 07:05:50 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 24 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c523t-e010a109d58e3df939ff0a9cd4f985c5b3fd71679ca3f789cd207b18838e4b3d3 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 To whom reprint requests should be addressed. e-mail: fuchs@esbs.u-strasb.fr or villani@ipbs.fr. I. Robert Lehman, Stanford University Medical Center, Stanford, CA |
| OpenAccessLink | https://doi.org/10.1073/pnas.93.24.13766 |
| PMID | 8943009 |
| PQID | 201311252 |
| PQPubID | 42026 |
| PageCount | 4 |
| ParticipantIDs | pnas_primary_93_24_13766 pubmedcentral_primary_oai_pubmedcentral_nih_gov_19419 crossref_primary_10_1073_pnas_93_24_13766 jstor_primary_40975 proquest_journals_201311252 pubmed_primary_8943009 proquest_miscellaneous_15778872 pnas_primary_93_24_13766_fulltext |
| ProviderPackageCode | RNA PNE |
| PublicationCentury | 1900 |
| PublicationDate | 1996-11-26 |
| PublicationDateYYYYMMDD | 1996-11-26 |
| PublicationDate_xml | – month: 11 year: 1996 text: 1996-11-26 day: 26 |
| PublicationDecade | 1990 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Washington |
| PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
| PublicationTitleAlternate | Proc Natl Acad Sci U S A |
| PublicationYear | 1996 |
| Publisher | National Academy of Sciences of the United States of America National Acad Sciences National Academy of Sciences The National Academy of Sciences of the USA |
| Publisher_xml | – name: National Academy of Sciences of the United States of America – name: National Acad Sciences – name: National Academy of Sciences – name: The National Academy of Sciences of the USA |
| References | e_1_3_2_26_2 e_1_3_2_27_2 e_1_3_2_28_2 e_1_3_2_29_2 e_1_3_2_20_2 e_1_3_2_21_2 e_1_3_2_22_2 e_1_3_2_23_2 e_1_3_2_24_2 e_1_3_2_25_2 Kornberg A (e_1_3_2_1_2) 1992 e_1_3_2_9_2 e_1_3_2_15_2 e_1_3_2_8_2 e_1_3_2_16_2 e_1_3_2_17_2 e_1_3_2_6_2 e_1_3_2_18_2 Goubin F (e_1_3_2_7_2) 1995; 10 e_1_3_2_19_2 e_1_3_2_30_2 e_1_3_2_10_2 e_1_3_2_5_2 e_1_3_2_11_2 e_1_3_2_4_2 e_1_3_2_12_2 e_1_3_2_3_2 e_1_3_2_13_2 e_1_3_2_2_2 e_1_3_2_14_2 |
| References_xml | – ident: e_1_3_2_8_2 doi: 10.1073/pnas.92.12.5356 – ident: e_1_3_2_16_2 doi: 10.1021/bi00660a027 – ident: e_1_3_2_13_2 doi: 10.1006/jmbi.1994.1125 – ident: e_1_3_2_12_2 doi: 10.1074/jbc.271.26.15386 – ident: e_1_3_2_9_2 doi: 10.1093/nar/20.24.6473 – ident: e_1_3_2_26_2 doi: 10.1016/S0021-9258(18)82201-3 – volume-title: DNA Replication year: 1992 ident: e_1_3_2_1_2 contributor: fullname: Kornberg A – ident: e_1_3_2_24_2 doi: 10.1016/S0092-8674(00)81039-9 – ident: e_1_3_2_2_2 doi: 10.1016/S0021-9258(17)42371-4 – ident: e_1_3_2_21_2 doi: 10.1073/pnas.91.16.7752 – ident: e_1_3_2_6_2 doi: 10.1126/science.8342022 – ident: e_1_3_2_14_2 doi: 10.1038/377649a0 – ident: e_1_3_2_28_2 doi: 10.1021/bi00144a021 – ident: e_1_3_2_10_2 doi: 10.1021/bi00169a018 – ident: e_1_3_2_17_2 doi: 10.1073/pnas.87.16.6087 – ident: e_1_3_2_18_2 doi: 10.1016/0022-2836(94)90010-8 – volume: 10 start-page: 2281 year: 1995 ident: e_1_3_2_7_2 publication-title: Oncogene contributor: fullname: Goubin F – ident: e_1_3_2_22_2 doi: 10.1074/jbc.270.36.21226 – ident: e_1_3_2_11_2 doi: 10.1038/369574a0 – ident: e_1_3_2_20_2 doi: 10.1038/294657a0 – ident: e_1_3_2_29_2 doi: 10.1128/mcb.13.1.533-542.1993 – ident: e_1_3_2_25_2 doi: 10.1016/S0021-9258(17)31859-8 – ident: e_1_3_2_19_2 doi: 10.1093/nar/22.13.2519 – ident: e_1_3_2_3_2 doi: 10.1016/0076-6879(95)62037-0 – ident: e_1_3_2_27_2 doi: 10.1002/j.1460-2075.1994.tb06826.x – ident: e_1_3_2_5_2 doi: 10.1128/MCB.16.5.2537 – ident: e_1_3_2_23_2 doi: 10.1016/0921-8777(93)90062-L – ident: e_1_3_2_4_2 doi: 10.1038/352544a0 – ident: e_1_3_2_30_2 doi: 10.1073/pnas.92.26.11975 – ident: e_1_3_2_15_2 doi: 10.1021/bi00764a017 |
| SSID | ssj0009580 |
| Score | 1.7448224 |
| Snippet | DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly... DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly... Hoffmann et al examined the effect of the asymmetry of DNA replication on the efficiency of translesion synthesis. Results suggest a role for additional... |
| SourceID | pubmedcentral proquest crossref pubmed pnas jstor |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 13766 |
| SubjectTerms | 2-Acetylaminofluorene Adducts Animals Antineoplastic Agents Base Sequence Biochemistry Biological Sciences Carcinogens Cell extracts CHO Cells Cisplatin Cricetinae Deoxyribonucleic acid DNA DNA Adducts DNA Damage DNA Replication DNA, Single-Stranded - chemistry DNA, Single-Stranded - isolation & purification DNA, Single-Stranded - metabolism Genetics Lesions Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides Platinum Templates, Genetic |
| Title | Fork-Like DNA Templates Support Bypass Replication of Lesions that Block DNA Synthesis on Single-Stranded Templates |
| URI | https://www.jstor.org/stable/40975 http://www.pnas.org/content/93/24/13766.abstract https://www.ncbi.nlm.nih.gov/pubmed/8943009 https://www.proquest.com/docview/201311252 https://search.proquest.com/docview/15778872 https://pubmed.ncbi.nlm.nih.gov/PMC19419 |
| Volume | 93 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB3RSki9IFqoGgrFSBzoIdk4zpePVWFVQK04UKk3K4ltdbXbbLTePfTGtX-zv4Sx40RdBBeumXESeWbiN57xC8DHOkdQm1EVNnURh2nFNIZUVYVS4nogeZxrZc87X17lF9fpt5vsxp_jNr6tsm3qWdQu7qJ2dut6K7u7ZjL0iU1-XJ5j4k35ZAd20D2HBH3k2S37UycJfnzTJPWVSXTkSddWJuIsStKIYljle_DckY_bXsQna1Lflmi5TlH_b7jzz_bJJ-vR9CW88ECSnPUvvA_PVHsA-z5UDfnk-aRPX8FmulzNw8VsrsjnqzNiyagWFmESs-ks-ib1fYcQmqzUWMsmS01wFqxLkvVthSq45s3dcHPfImY0M0NQzW40LFRot0vsTvrjr4fx7q_hevrl5_lF6P-2EDaYjK5DhZlZRWMus1IxqTnjWscVb2SqeZk1Wc20LGzRpkGDFiUKkrioaVmyUqU1k-wQdttlq46AKDSy1FSXtWT2R8C80ogqELtwxGsypwGcDvMtup5UQ7hieMGEnXXBmUhS4awUwIEzyKhoCbqyAI6c4nBxa8CHf4mE9g01ARwPZhU-Zo1IHPVQkiUBvB-lGGy2glK1arkxgmaF7b5EjcPeBcaneE8KINvyjVFuWby3Jejcjs3bOfOb_xx3DHuujZzSMMnfwu56tVHvECWt6xPMD75-P3HR8RtQLRVA |
| link.rule.ids | 230,315,730,783,787,888,27936,27937,53804,53806 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFH6CIcQuiA2mhQEzEgd2SBrH-eXjNKgKrBWHTdrNSmJbq9qlUdMeduPKv8lfwrPjRCuCC9e-57Tye6_-7Pf5C8CHMkVQm1DlV2UW-nHBNJZUUfhS4nogeZhqZe47T2fp5Dr-epPcuHvcraNV1lU5D-rlXVDPby23srmrRj1PbPR9eoEbb8pHj-EJVmsY91v0QWk37-6dRPj3G0ex601iKo-aumgDzoIoDigWVroPT638uGEjPliVOmKiUTtF_78hzz8JlA9WpPELeO6gJDnvfvIBPFL1IRy4Ym3JR6coffYStuPVeuEv5wtFPs3OiZGjWhqMSdptY_A3Ke8bBNFkrYZuNllpgvNgkpJsbgt0wVVvYYe39zWixnbeEnQzRw1L5ZsDE3OW_uvHz-Hpr-B6_PnqYuK79y34FW5HN77CvVlBQy6TXDGpOeNahwWvZKx5nlRJybTMTNumwpBmORqiMCtpnrNcxSWT7Aj26lWtjoEoDLPUVOelZOZVwLzQiCsQvXBEbDKlHpz18y2aTlZD2HZ4xoSZdcGZiGJho-TBoQ3I4GgkuhIPjq1j_-HOgPf_MgntKDUenPRhFa5qWxFZ8aEoiTw4HaxYbqaHUtRqtW0FTTLDv0SPoy4Fhm9xmeRBspMbg93oeO9aML2tnrdN59f_Oe4Unk2uppfi8svs2wnsW1I5pX6UvoG9zXqr3iJm2pTvbI38BnvqF50 |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BEagXRAtVQ4EaiQM95OE4Lx-rllV5dNUDlXqzkthWV7vNRpvdQ29c-Zv8EsaOE3URXLjujJOVZyb-7PnyBeBDlSGoTany6yqP_KRkGkuqLH0pcT2QPMq0Mu87X06zi-vky016444uOkerbOpqFjSLu6CZ3VpuZXtXhwNPLLy6PMONN-VhK3X4GJ5gxUbZsE0f1XaL_t2TGB_BSZy4_iSmc9g2ZRdwFsRJQLG4sl14aiXIDSPxwcrUkxON4in6_w19_kmifLAqTV7AcwcnyWn_t_fgkWr2Yc8VbEc-OlXpk5ewmSxXc38xmytyPj0lRpJqYXAm6TatweCkum8RSJOVGjvaZKkJzoVJTLK-LdEFV765Hd7dN4gcu1lH0M0cNyyUbw5NzHn6rx8_x6u_guvJp-9nF7775oJf45Z07Svcn5U04jItFJOaM651VPJaJpoXaZ1WTMvctG5qDGteoCGO8ooWBStUUjHJDmCnWTbqEIjCUEtNdVFJZj4HzEuN2AIRDEfUJjPqwckw36LtpTWEbYnnTJhZF5yJOBE2Sh7s24CMjkamK_Xg0DoOP24NeP8vk9COVuPB0RBW4Sq3E7EVIIrT2IPj0YolZ_ooZaOWm07QNDccTPQ46FNgvIvLJA_SrdwY7UbLe9uCKW41vW1Kv_7Pccfw7Op8Ir59nn49gl3LK6fUj7M3sLNebdRbhE3r6p0tkd9xgxiw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Fork-like+DNA+templates+support+bypass+replication+of+lesions+that+block+DNA+synthesis+on+single-stranded%E2%80%89templates&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Hoffmann%2C+Jean-S%C3%A9bastien&rft.au=Pillaire%2C+Marie-Jeanne&rft.au=Lesca%2C+Claire&rft.au=Burnouf%2C+Dominique&rft.date=1996-11-26&rft.issn=0027-8424&rft.eissn=1091-6490&rft.volume=93&rft.issue=24&rft.spage=13766&rft.epage=13769&rft_id=info:doi/10.1073%2Fpnas.93.24.13766&rft.externalDBID=n%2Fa&rft.externalDocID=10_1073_pnas_93_24_13766 |
| thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F93%2F24.cover.gif |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F93%2F24.cover.gif |