Development of a nucleic Acid extraction procedure for simultaneous recovery of DNA and RNA from diverse microbes in water

• Published in: Pathogens (Basel) Vol. 4; no. 2; pp. 335 - 354
• Main Authors: Hill, Vincent R, Narayanan, Jothikumar, Gallen, Rachel R, Ferdinand, Karen L, Cromeans, Theresa, Vinjé, Jan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.05.2015; MDPI
• Subjects: Acids; Bacteria; Deoxyribonucleic acid; Disease control; DNA; Drinking water; Enzymes; extraction; Gram-positive bacteria; nucleic acid; Nucleic acids; Parasites; Pathogens; PCR facilitators; Protozoa; purification; RNA; Sample preparation; Water analysis; Water control; Water quality; Water sampling; water testing; Water treatment; Water treatment plants; Atlanta Georgia; United States > US; RNA; DNA; purification; PCR facilitators; drinking water; nucleic acid; extraction; water testing

Drinking and environmental water samples contain a diverse array of constituents that can interfere with molecular testing techniques, especially when large volumes of water are concentrated to the small volumes needed for effective molecular analysis. In this study, a suite of enteric viruses, bact...