Cryopreservation of Induced Pluripotent Stem Cell-Derived Dopaminergic Neurospheres for Clinical Application

• Published in: Journal of Parkinson's disease Vol. 12; no. 3; pp. 871 - 884
• Main Authors: Hiramatsu, Satoe, Morizane, Asuka, Kikuchi, Tetsuhiro, Doi, Daisuke, Yoshida, Kenji, Takahashi, Jun
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.01.2022; IOS Press BV; IOS Press
• Subjects: 6-Hydroxydopamine; Animals; Cell Differentiation; Cell suspensions; Cell therapy; Cryopreservation; Cryopreservation - methods; Dopamine - metabolism; Dopamine receptors; Dopaminergic Neurons - metabolism; Fetuses; Freezing; Humans; Induced Pluripotent Stem Cells - metabolism; Movement disorders; Neurodegenerative diseases; Neurospheres; Oxidopamine - metabolism; Parkinson Disease - therapy; Parkinson's disease; Pluripotency; Rats; Research Report; Rotational behavior; Stem cells; Thawing; Transplantation; induced pluripotent stem cells; dopaminergic neuron; Cryopreservation; cell-based therapy; Parkinson’s disease; neurosphere

Background: Pluripotent stem cell (PSC)-derived dopaminergic (DA) neurons are an expected source of cell therapy for Parkinson’s disease. The transplantation of cell aggregates or neurospheres, instead of a single cell suspension has several advantages, such as keeping the 3D structure of the donor...