Comparison of three culture media for the establishment of melanoma cell lines

• Published in: Cytotechnology (Dordrecht) Vol. 62; no. 5; pp. 403 - 412
• Main Authors: Pandolfino, M. C, Saïagh, S, Knol, A. C, Dréno, B
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.10.2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Adoptive immunotherapy; Antibodies; Antigen (tumor-associated); Antigens; Biochemistry; Biological and medical sciences; Biomedicine; Biotechnology; Cell culture; Cell surface; Cell-based therapy; Chemistry; Chemistry and Materials Science; Culture media; Cytology; Fetal calf serum; Fundamental and applied biological sciences. Psychology; Good Manufacturing Practice; Immunotherapy; Laboratories; Lymph nodes; Lymphocytes; Melanoma; Original Research; Serum-free cell culture medium; Tumor cell line growth; Tumor cell lines; Tumors; United Kingdom > UK; France; United States > US; Serum-free cell culture medium; Cell-based therapy; Melanoma; Tumor cell line growth; Culture medium; Cell proliferation; Cell culture; Serum free medium; Cell therapy; Malignant tumor; Cell line; Malignant melanoma; Tumor cell; Comparative study; Cancer

Melanoma cell lines are useful tools for the analysis of tumor-specific lymphocytes which are injected to patients treated by adoptive immunotherapy. So they have been established previously (with an efficacy of 47%) in Roswell Park Memorial Institute (RPMI) medium enriched with fetal calf serum (FC...