CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 111; no. 6; pp. 2158 - 2163
• Main Authors: Yang, Yunfan, Liu, Min, Li, Dengwen, Ran, Jie, Gao, Jinmin, Suo, Shaojun, Sun, Shao-Cong, Zhou, Jun
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 11.02.2014; National Acad Sciences
• Subjects: Animals; Antibodies; Antigens, Nuclear - metabolism; Biological Sciences; Caco 2 cells; Cell division; Cellular immunity; Deubiquitinating Enzyme CYLD; Dynactin Complex; dynein ATPase; Dyneins - metabolism; Epithelial cells; Gender equity; Gene expression; HeLa Cells; homeostasis; Humans; Mice; Mice, Knockout; Microscopy, Fluorescence; Microtubule-Associated Proteins - metabolism; Microtubules; Microtubules - metabolism; mitosis; Mitotic spindle apparatus; Nuclear Matrix-Associated Proteins - metabolism; Proteins; Small interfering RNA; Spindle Apparatus - physiology; Tissues; Tumor Suppressor Proteins - physiology; Tumors; 3D cell culture; cell cycle; cell cortex; knockout mouse; ubiquitin

Oriented cell division is critical for cell fate specification, tissue organization, and tissue homeostasis, and relies on proper orientation of the mitotic spindle. The molecular mechanisms underlying the regulation of spindle orientation remain largely unknown. Herein, we identify a critical role...