Cytometry by time-of-flight immunophenotyping identifies a blood Sjögren's signature correlating with disease activity and glandular inflammation

• Published in: Journal of allergy and clinical immunology Vol. 137; no. 6; pp. 1809 - 1821.e12
• Main Authors: Mingueneau, Michael, Boudaoud, Saida, Haskett, Scott, Reynolds, Taylor L., Nocturne, Gaetane, Norton, Elizabeth, Zhang, Xueli, Constant, Myrtha, Park, Daniel, Wang, Wenting, Lazure, Thierry, Le Pajolec, Christine, Ergun, Ayla, Mariette, Xavier
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2016; Elsevier Limited
• Subjects: Adult; Allergy and Immunology; Antigens; autoimmunity; biomarker discovery; Biomarkers; Biopsy; Blood; CD8-Positive T-Lymphocytes; Cloning; Cluster Analysis; Computational Biology - methods; cytometry by time-of-flight; Disease; Enzymes; Exocrine glands; Female; Flow Cytometry; Humans; Immunoglobulins; Immunohistochemistry; immunophenotyping; Immunophenotyping - methods; Male; mass cytometry; Middle Aged; patient stratification; Plasma Cells; Reproducibility of Results; Salivary Glands - metabolism; Salivary Glands - pathology; Salivary Glands, Minor - metabolism; Salivary Glands, Minor - pathology; Sjogren's Syndrome - diagnosis; Sjögren's syndrome; therapeutic target; IHC; patient stratification; biomarker discovery; FS; DMEM; ESSDAI; pSS; immunophenotyping; AECG; LASSO; AID; cytometry by time-of-flight; SSA; LSG; SSB; therapeutic target; Bm; pDC; autoimmunity; RF; Sjögren's syndrome; MXA; mass cytometry; CyTOF; NK; Immunohistochemistry; Sjögren's syndrome B antigen; Autoimmune disease; Plasmacytoid dendritic cell; Labial salivary gland; Myxovirus resistance gene A; Dulbecco modified Eagle medium; Sjögren's syndrome A antigen; American European Consensus criteria; Memory B; Focus score; EULAR Sjögren's syndrome disease activity score; Least Absolute Shrinkage and Selection operator; Rheumatoid factor; Natural killer; Primary Sjögren's syndrome

Mass cytometry has recently emerged as a promising tool for clinical research. However, few studies have demonstrated its benefit for patient stratification and biomarker identification. Primary Sjögren's syndrome (pSS) is a prototype of chronic autoimmune disease, the pathogenesis of which rem...