Carbohydrate-based vaccines with a glycolipid adjuvant for breast cancer

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 110; no. 7; pp. 2517 - 2522
• Main Authors: Huang, Yen-Lin, Hung, Jung-Tung, Cheung, Sarah K. C., Lee, Hsin-Yu, Chu, Kuo-Ching, Li, Shiou-Ting, Lin, Yu-Chen, Ren, Chien-Tai, Cheng, Ting-Jen R., Hsu, Tsui-Ling, Yu, Alice L., Wu, Chung-Yi, Wong, Chi-Huey
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 12.02.2013; National Acad Sciences
• Subjects: adjuvants; Animals; Antibodies; Antigens; Antigens, Tumor-Associated, Carbohydrate - administration & dosage; Antigens, Tumor-Associated, Carbohydrate - chemistry; Antigens, Tumor-Associated, Carbohydrate - immunology; Antigens, Tumor-Associated, Carbohydrate - pharmacology; antiserum; Bacterial Proteins - immunology; Biological Sciences; Breast cancer; breast neoplasms; Breast Neoplasms - immunology; Breast Neoplasms - prevention & control; Cancer Vaccines - chemistry; Carbohydrates; Carrier proteins; clinical trials; Epitopes; Female; Flow Cytometry; Glycolipids; Hemocyanins; Immune response; Immune Sera - analysis; Immunization; immunoglobulin G; Immunoglobulin G - immunology; Immunoglobulins; Mice; Microarray Analysis; microarray technology; Molecular Structure; neoplasm cells; Neoplastic Stem Cells - metabolism; Physical Sciences; Polysaccharides; Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stage-Specific Embryonic Antigens - immunology; Stem cells; tetanus; Tetanus Toxoid; Vaccination; vaccine development; Vaccines

Globo H (GH) is a hexasaccharide specifically overexpressed on a variety of cancer cells and therefore, a good candidate for cancer vaccine development. To identify the optimal carrier and adjuvant combination, we chemically synthesized and linked GH to a carrier protein, including keyhole limpet he...