Fluorescent proteins in animal cells for process development: Optimization of sodium butyrate treatment as an example

• Published in: Biotechnology and bioengineering Vol. 77; no. 5; pp. 528 - 537
• Main Authors: Hunt, Lisa, Batard, Pascal, Jordan, Martin, Wurm, Florian M.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 05.03.2002; Wiley
• Subjects: Animal cells; Animals; Biological and medical sciences; Biotechnology; Butyrates - pharmacology; Cell Count; Cell Culture Techniques - methods; CHO cells; CHO Cells - cytology; CHO Cells - physiology; Cricetinae; Dose-Response Relationship, Drug; Eukaryotic cell cultures; fluorescent proteins; Fundamental and applied biological sciences. Psychology; Gene Expression; GFP; Glucose - metabolism; Green Fluorescent Proteins; Indicators and Reagents - metabolism; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Methods. Procedures. Technologies; Microscopy, Fluorescence - methods; Miscellaneous; process development tool; recombinant protein production; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; sodium butyrate; Transgenes; YFP; Productivity; Cell culture; Animal; Production; Green fluorescent protein; Fluorescence; Measurement method; Recombinant protein; Gene expression; CHO cell line; Optimization

Fluorescent proteins expressed in mammalian cells can be quantified quickly and noninvasively with a standard fluorescence plate reader. We have previously exploited this quality in cell growth assessment (Hunt et al., 1999b). In this work, different CHO cell lines constitutively expressing fluoresc...