Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons

• Published in: Glia Vol. 58; no. 10; pp. 1168 - 1176
• Main Authors: Halim, Nader D., Mcfate, Thomas, Mohyeldin, Ahmed, Okagaki, Peter, Korotchkina, Lioubov G., Patel, Mulchand S., Jeoung, Nam Ho, Harris, Robert A., Schell, Michael J., Verma, Ajay
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2010
• Subjects: Animals; Astrocytes; Astrocytes - enzymology; Astrocytes - metabolism; Brain; Brain - enzymology; Brain - metabolism; Cell culture; Cells, Cultured; Coculture Techniques; dehydrogenase; Dephosphorylation; dichloroacetate; Electrophoresis, Gel, Two-Dimensional; Enzymatic activity; Glucose metabolism; Glycolysis; Immunoblotting; Immunohistochemistry; L-Lactate Dehydrogenase - metabolism; lactate; Lactic acid; Lactic Acid - metabolism; Mitochondria; Neurons; Neurons - enzymology; Neurons - metabolism; Oxidation; Phosphorylation; Pyruvate dehydrogenase (lipoamide); Pyruvate Dehydrogenase Complex - metabolism; Pyruvic acid; Rats; Rats, Sprague-Dawley; Tricarboxylic acid cycle

Glucose metabolism in nervous tissue has been proposed to occur in a compartmentalized manner with astrocytes contributing largely to glycolysis and neurons being the primary site of glucose oxidation. However, mammalian astrocytes and neurons both contain mitochondria, and it remains unclear why in...