CytoNorm: A Normalization Algorithm for Cytometry Data

• Published in: Cytometry. Part A Vol. 97; no. 3; pp. 268 - 278
• Main Authors: Van Gassen, Sofie, Gaudilliere, Brice, Angst, Martin S., Saeys, Yvan, Aghaeepour, Nima
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.03.2020; Wiley Subscription Services, Inc
• Subjects: Algorithms; barcoding; Clustering; computational cytometry; Datasets; Evaluation; Flow cytometry; Immune system; mass cytometry; Mathematical analysis; normalization; Original; Phenotyping; Proteomics; Sample preparation; Transformations; barcoding; computational cytometry; mass cytometry; normalization

High‐dimensional flow cytometry has matured to a level that enables deep phenotyping of cellular systems at a clinical scale. The resulting high‐content data sets allow characterizing the human immune system at unprecedented single cell resolution. However, the results are highly dependent on sample...