High-resolution visualization of pial surface vessels by flattened whole mount staining

• Published in: iScience Vol. 26; no. 4; p. 106467
• Main Authors: Xu, Yanying, Zhang, Jiasheng, Lee, Heon-Woo, Zhang, Guogang, Bai, Yongping, Simons, Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.04.2023; Elsevier
• Subjects: Medical imaging; Neuroscience; Vascular anatomy; Vascular anatomy; Neuroscience; Medical imaging

Understanding development of the cerebral vasculature is essential for the central nervous system (CNS) research and therapeutic developments. Here, we developed a simple, convenient, and fast method—the flattened cortex whole mount (FCWM) technique—for imaging of pial cerebral vessels. FCWM involve...