Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

• Published in: STAR protocols Vol. 4; no. 3; p. 102468
• Main Authors: Ichikawa, Takehiko, Alam, Mohammad Shahidul, Penedo, Marcos, Matsumoto, Kyosuke, Fujita, Sou, Miyazawa, Keisuke, Furusho, Hirotoshi, Miyata, Kazuki, Nakamura, Chikashi, Fukuma, Takeshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2023; Elsevier
• Subjects: AFM; Atomic Force Microscopy; Cell Biology; Cell Membrane - chemistry; Microscopy; Microscopy, Atomic Force - methods; Nanotechnology - methods; Protocol; AFM; Atomic Force Microscopy; Microscopy; Cell Biology

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures inside living cells. The protocol consists of...